Journal of Kermanshah University of Medical Sciences
Peer-reviewed Medical Quarterly
e-ISSN 2588-2570
p-ISSN 2588-2562
Outline
Inhibitory effects of ethanolic, methanolic and hexanolic extracts of propolis on the activity and structure of tyrosinase
Authors
Nematollah Gheibi1, Mohammad Sofiabadi2,*, Sharam Rastak3, Negar Taherkhani4, Zahra Mohammadi5, Zahra Golpour3
1Cellular and Molecular Research Center, Qazvin University of Medical Sciences, Qazvin, Iran
2Dept. of Physiology, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran
3Dept. of Anesthesiology, School of Paramedical Sciences, Qazvin University of Medical Sciences, Qazvin, Iran
4Tehran Science and Research Branch, Islamic Azad University, Tehran, Iran
5Student Research Committee, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran
*Corresponding Author: Corresponding author: Mohammad Sofiabadi, Dept. of Physiology, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran, Tel: +98 9128811731 Email: [email protected]
Published online:Dec 19, 2016
Article type:Research Article
Received:Aug 06, 2016
Accepted:Dec 03, 2016
How to Cite:Gheibi N, Sofiabadi M, Rastak S, Taherkhani N, Mohammadi Z, et al. Inhibitory effects of ethanolic, methanolic and hexanolic extracts of propolis on the activity and structure of tyrosinase. J Kermanshah Univ Med Sci. 2016;20(3):e69690. doi: https://doi.org/10.22110/jkums.v20i3.2920
Abstract
Introduction: Tyrosinase is a key enzyme in the biosynthesis of melanin, which plays a crucial role in determining mammal’s skin and hair color. In this experimental study, the inhibitory effect of different extracts of propolis were investigated on tyrosinase activity.
Methods: Tyrosinase activity was measured in the presence of ethanolic, methanolic and hexanolic extracts of propolis by using thermal denaturation (Catecholase and cresolase reaction). Also the tyrosinase stability was examined in the presence of the extracts with the chemical (urea) denaturation method. Data were analyzed with SPSS software using ANOVA and Tokey post hoc test.
Results: Tm was 56.1 °C for tyrosinase in the absence of the extract and 47.4, 53.4 and 40.2 °C in the presence of ethanolic, methanolic and hexanolic extracts of propolis, respectively. Also ΔG 25 °C values were obtained 16.03 in the absence of the extracts and 13.5 (p<0.05), 15.3 and 13.3 (p<0.05) kJ/mol, respectively in the presence of ethanolic, methanolic and hexanolic extracts of propolis. In chemical denaturation, Cm was obtained 3.11 in the absence of the extracts and 15.8 (p<0.01), 11.9 (p<0.05), and 12.5(p<0.05) mM, respectively in the presence of ethanolic, methanolic and hexanolic extracts of propolis.
Conclusion: Ethanolic, methanolic and hexanaloic extracts of propolis are appropriate inhibitors for the tyrosinase. These reduce the thermal and chemical tyrosinase stability.
References
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Copyright
© 2016, Journal of Kermanshah University of Medical Sciences. This open-access article is available under the Creative Commons Attribution-NonCommercial 4.0 (CC BY-NC 4.0) International License (https://creativecommons.org/licenses/by-nc/4.0/), which allows for the copying and redistribution of the material only for noncommercial purposes, provided that the original work is properly cited.
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