Antidiabetic Potential Yacon (Smallanthus sonchifolius (Poepp.) H. Rob.) Leaf Extract via Antioxidant Activities, Inhibition of α-glucosidase, α- amylase, G-6-Pase by In Vitro Assay

Wahyu Widowati; Rita Tjokropranoto; Roro Wahyudianingsih; Fen Tih; Lisawati Sadeli; Hanna Widya Kusuma; Nerissa Arviana Fuad; Ermi Girsang; Faustina Audrey Agatha

doi:10.4103/jrptps.JRPTPS_3_21

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https://doi.org/10.4103/jrptps.JRPTPS_3_21

Antidiabetic Potential Yacon (Smallanthus sonchifolius (Poepp.) H. Rob.) Leaf Extract via Antioxidant Activities, Inhibition of α-glucosidase, α- amylase, G-6-Pase by In Vitro Assay

Author(s):

Wahyu Widowati^1,*,

Rita Tjokropranoto¹,

Roro Wahyudianingsih¹,

Fen Tih¹,

Lisawati Sadeli¹,

Hanna Widya Kusuma²,

Nerissa Arviana Fuad¹,

Ermi Girsang³,

Faustina Audrey Agatha⁴

1Faculty of Medicine, Maranatha Christian University, Jl. Prof. drg. Surya Sumantri no. 65 Bandung 40164, West Java, Indonesia

2Aretha Medika Utama, Biomolecular and Biomedical Research Center, Jl. Babakan Jeruk 2 no.9 Bandung 40163, West Java, Indonesia

3Faculty of Medicine, Universitas Prima Indonesia, Jl. Belanga No. 1 Simp. Ayahanda, Medan 20118, North Sumatera, Indonesia

4Faculty of Technobiology, Universitas Katolik Indonesia Atmajaya, Jl. Cisauk, BSD, Tangerang Selatan 15345, Banten, Indonesia

Journal of Reports in Pharmaceutical Sciences:Vol. 10, issue 2; 247-55

Published online:Dec 17, 2021

Article type:Research Article

Received:Jan 05, 2021

Accepted:Apr 18, 2021

How to Cite:Widowati W, Tjokropranoto R, Wahyudianingsih R, Tih F, Sadeli L, et al. Antidiabetic Potential Yacon (Smallanthus sonchifolius (Poepp.) H. Rob.) Leaf Extract via Antioxidant Activities, Inhibition of α-glucosidase, α- amylase, G-6-Pase by In Vitro Assay. J Rep Pharm Sci. 2021;10(2):e146960. doi: https://doi.org/10.4103/jrptps.JRPTPS_3_21

Abstract

Background: Diabetes is a chronic disease characterized by glucose levels and results in impaired insulin secretion. This disorder has triggered oxidative stress and excess free radicals condition. Smallanthus sonchifolius is a traditional medicine that acts as a diabetic therapy. This research aims to bring out the antidiabetic and antioxidant potential of S. sonchifolius extract (SSE).
Materials and Methods: This study was conducted to measure the qualitative phytochemical identification, antioxidant and anti-diabetic activity of SSE. The antioxidant assay was carried out using 2,2-diphenyl-1-picrylhydrazine (DPPH)- scavenging activity, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS)-scavenging and hydrogen peroxide (H2O2)-reducing activity assays, ferric reducing antioxidant power (FRAP) potency, while anti-diabetic activity of SSE assay was carried out using inhibitory of α-amylase, α-glucosidase, and Glucose-6-Phosphatase (G-6-Pase).
Results: SSE contained phenols, flavonoids, steroids/triterpenoids, saponins, tannins, and alkaloids. The antioxidant and antidiabetic activities of samples were calculated based on median inhibitory concentration (IC50). The IC50 values of SSE antioxidant, respectively, were DPPH (IC50 = 62.72 μg/mL), ABTS (IC50 = 61.03 μg/mL), H2O2 (IC50 = 438.36 μg/mL), the highest FRAP activity was 125.31 μM Fe(II)/μg extract at a concentration level of SSE 50 μg/mL. The IC50 values of SSE antidiabetic were α-amylase inhibition (IC50 = 37.86 μg/mL), α-glucosidase inhibition (IC50 = 90.41 μg/ mL), and G-6-Pase inhibition (IC50 = 98.07 μg/mL), respectively.
Conclusions: SSE has antidiabetic potential through antioxidant activities and α-glucosidase, α-amylase, and G-6-Pase inhibition activities.

Keywords

Antioxidants

diabetes

Smallanthus sonchifolius

α-amylase

α-glucosidase

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Author(s):

Wahyu Widowati:[PubMed][Scholar]
Rita Tjokropranoto:[PubMed][Scholar]
Roro Wahyudianingsih:[PubMed][Scholar]
Fen Tih:[PubMed][Scholar]
Lisawati Sadeli:[PubMed][Scholar]
Hanna Widya Kusuma:[PubMed][Scholar]
Nerissa Arviana Fuad:[PubMed][Scholar]
Ermi Girsang:[PubMed][Scholar]
Faustina Audrey Agatha:[PubMed][Scholar]

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Antidiabetic Potential Yacon (Smallanthus sonchifolius (Poepp.) H. Rob.) Leaf Extract via Antioxidant Activities, Inhibition of α-glucosidase, α- amylase, G-6-Pase by In Vitro Assay

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