Cloning of glucose oxidase gene in PKK233-3

Introduction: Glucose oxidase (GO) has found a variety of industrial applications such as food, chemical and personal care industries. However one of the most important application of GO is used as diagnostic kits. The aim of study was isolation of GO gene from a recombinant vector (PET21aGO) and sub cloning and expression in PKK233-3 vector. Materials and Methods: Recombinant PET21a GO was extracted from E.coli DH5α and was digested with Restriction Enzymes BamHI, HindШ then isolated GO gene (1.8kb) and cloned in PKK233-3. PKK233-3GO was transformed in to E.coli DH5α. Results: Our data demonstrated that the GO gene has expected size in agarose gel electrophoresis and also the cloned Go has a correct size after restriction analysis. Conclusion: The GO gene was cloned in prokaryotic host. This is a report of cloning of GO gene in Iran that can be used for further cloning of that gene in expression vectors for production of recombinant Enzyme.