Effects of Submaximal Endurance Training and Vitamin DR3 RSupplementation on Pain Threshold in Diabetic Rats

Background : According to beneficial effects of endurance training and vitamin D3 in diabetes mellitus, purpose of this study is effects submaximal endurance training and vitamin D3 supplementation on pain threshold in streptozotocin induced diabetic rats.
Materials and Methods : Male Wistar rats (250±20 g, N=40) were made diabetic by streptozotocin (60 mg/kg, subcutaneously). 72 h after injection diabetes induction was confirmed by tail vein blood glucose concentration (>300 mg/dl). Then animals were divided to five groups: diabetic control (DC), diabetic trained (DT), diabetic -vitamin D (DD), diabetic trained and vitamin D (DTD), and control (C). Animals were submitted to endurance training by treadmill and vitamin D3 treatment (twice aweek, intrapretonally) for 4 weeks. 48 h after at the end of exercise and treatment protocol, we used tail-flick to assess the effects of training and vitamin D3 on thermal pain threshold. We used one way ANOVA statistical analysis to compare differences between groups, significance level of p<0.05 was considered.
Results : Diabetic induced hyperalgesia were decreased significantly by vitamin D but not 4 weeks endurance exercise training. Concurrent effects of training and vitamin D on thermal pain threshold were not significantly higher than vitamin D effects alone.
Conclusion : It is concluded that vitamin D administration given at the time of diabetes induction may be able to restore thermal hyperalgesia. But effects of endurance exercise training needs to more investigation in diabetic rats.