The Study of “Dihydropyrimidinase Related Proteins (DRPs)” Expression Changes Influence in Malignant Astrocytoma Brain Tumor

Mehdi  Pooladi; Mostafa Rezaei-Tavirani; Mehrdad Hashemi; Saeed Hesami-Tackallou; Solmaz Khaghani-Razi-Abad; Afshin Moradi; Ali Reza Zali; Masoumea Mousavi; Azadeh Rakhshan; Leila Firozi-dalvand; Roghaye Omidi

International Journal of Cancer Management

The Official Journal of Cancer Research Center (CRC), Shahid Beheshti University of Medical Sciences

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Abstract
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The Study of “Dihydropyrimidinase Related Proteins (DRPs)” Expression Changes Influence in Malignant Astrocytoma Brain Tumor

Author(s):

Mehdi Pooladi^1,*,

Mostafa Rezaei-Tavirani²,

Mehrdad Hashemi³,

Saeed Hesami-Tackallou⁴,

Solmaz Khaghani-Razi-Abad¹,

Afshin Moradi⁵,

Ali Reza Zali⁶,

Masoumea Mousavi²,

Azadeh Rakhshan⁵,

Leila Firozi-dalvand²,

Roghaye Omidi²

1Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran; Dept. of Biology, School of Basic Sciences, Science and Research Branch, Islamic Azad University, Tehran, Iran

2Proteomics Research Center, Faculty of Paramedical Sciences, Shahid Beheshti University of Medical Sciences, Tehran, Iran

3Dept. of Molecular Genetics, Tehran Medical Branch, Islamic Azad University Tehran, Iran 4. Dept. of Biology, Garmsar Branch, Islamic Azad University, Garmsar, Andorra

4Dept. of Biology, Garmsar Branch, Islamic Azad University, Garmsar, Iran

5Dept. of Pathology, Shohada Hospital, Shahid Beheshti University Of Medical Science, Tehran, Iran

6Dept. of Neurosurgery, Shohada Hospital, Shahid Beheshti University of Medical Sciences, Tehran, Iran

International Journal of Cancer Management:Vol. 7, issue 3; e80540

Published online:Sep 30, 2014

Article type:Research Article

Received:Jan 27, 2014

Accepted:Mar 25, 2014

How to Cite:Pooladi M, Rezaei-Tavirani M, Hashemi M, Hesami-Tackallou S, Khaghani-Razi-Abad S, et al. The Study of “Dihydropyrimidinase Related Proteins (DRPs)” Expression Changes Influence in Malignant Astrocytoma Brain Tumor.Int J Cancer Manag.2014;7(3):e80540.

Abstract

Background: Dihydropyrimidinase Related Proteins (DRPs) have known homologous to the Collapsing Response Mediator Proteins (CRMPs). The DRP gene family has comprised four members, DRP 1, 2, 3, and 4, all out of which have considered to be involved in axonal outgrowth and path-finding.

Methods: The protein has extracted from tumor, normal brain tissues, and then the protein purity has evaluated by Bradford test and spectrophotometric methods. In this study, proteins has separated by Two-Dimensional Gel (2DG) electrophoresis method and then spots have analyzed and compared using statistical data and specific software (Progenesis Same Spots).Spots have identified by pH isoelectric, molecular weights and data banks.

Results: The 2D gel has shown 800 spots totally. Two spots have reported for DRP2, and one spot has reported for DRP3 in the human brain proteome, that have differed in pH isoelectric, and Molecular Weights values.

Conclusion: This protein family has involved in neuronal differentiation and axonal guidance, and abundantly influenced in the developing brain, but their expression persisted into adulthood. DRP2 has regulated by phosphorylation, Glycogen synthase kinase 3, regulate phosphorylation of DRP2 an inactive from, and induced neuronal polarity

Keywords

Asterocytoma

Proteomics

DRP and 2DG Electrophoresis

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