The Investigation of Heat Shock Protein (HSP70) Expression Change in Human Brain Asterocytoma Tumor

Background: Asterocytoma is the most common primary human brain tumor which has the most lethal hazard among tumors in human Central Nervous System. Heat Shock Proteins play an active role in cancer cells pathways through their effects on tumor cell proliferation, tumor differentiation, malignancy, metastasis, and cell death. HSP70 is a 70 KD protein that its expression change has been reported in various kinds of cancers. Here a proteomic activity for molecular diagnosis of Asterocytomas tumors is designed.

Methods: Proteins of tumor and normal brain tissues were extracted and then evaluated by Bradford test. In this study, the proteins were separated by 2DG Electrophoresis method, and then the spots were analyzed and compared using statistical data and specific software, after providing 3D images of spots alteration. Spots were identified by Isoelectric point (pI), molecular weights and data banks.

Results: As a result, the 2D gel showed totally 800 spots. HSP70 is one of down regulated proteins.

Conclusion: As conclusion, alteration of HSP70 expression was detected in Asterocytomas which has an important role in tumor survival and development, response to cell stress and induces apoptosis in cells. Three kinds of HSP70 are known in different cancers: HSP70-1, HSP70-2 and HSP70-8 which affect limitation of apoptosis system in cancer cells.