Method Validation for the One-Month Stability Study of trans–Resveratrol in Human Plasma

Milad Iranshahy; Amir Hooshang Mohammadpour; Mohammad Hassanzadeh-Khayyat; Mehrdad Iranshahi

doi:10.17795/jjnpp-9566

Jundishapur Journal of Natural Pharmaceutical Products

The Official Journal of Ahvaz Jundishapur University of Medical Sciences

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https://doi.org/10.17795/jjnpp-9566

Method Validation for the One-Month Stability Study of trans–Resveratrol in Human Plasma

Author(s):

Milad Iranshahy^{1, 2},

Amir Hooshang Mohammadpour³,

Mohammad Hassanzadeh-Khayyat⁴,

Mehrdad Iranshahi^{1, 2,*}

1Biotechnology Research Center, Mashhad University of Medical Sciences, Mashhad, IR Iran

2Department of Pharmacognosy, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IR Iran

3Department of Pharmacodynamy and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IR Iran

4Department of Medicinal Chemistry, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, IR Iran

Jundishapur Journal of Natural Pharmaceutical Products:Vol. 8, issue 2; e94152

Published online:May 04, 2013

Article type:Research Article

Received:May 20, 2019

Accepted:Jan 30, 2013

How to Cite:Milad IranshahyAmir Hooshang MohammadpourMohammad Hassanzadeh-KhayyatMehrdad IranshahiMethod Validation for the One-Month Stability Study of trans–Resveratrol in Human Plasma.Jundishapur J Nat Pharm Prod.8(2):e94152.https://doi.org/10.17795/jjnpp-9566.

Abstract

Background: trans-Resveratrol (t-Res) is a natural phenolic compound with various biological activities such as antioxidant, anticancer, cancer chemopreventive, cardioprotective, and neuroprotective.

Objectives: There is no comprehensive study about the stability of t-Res in human plasma. With respect to extensive use of t-Res in clinical trials and pharmacokinetic studies, in this study, we aimed to measure the plasma stability of t-Res at different conditions of lighting, pH, and temperature, and in the presence of routine antioxidant agents (BHT and VIT C).

Materials and Methods: t-Res stability in human plasma was evaluated at different conditions of lighting, pH and temperature, and in the presence of routine antioxidant agents (BHT and VIT C), and quantitatively determined using HPLC-UV method at 306 nm.

Results: Our findings revealed that t-Res was quite stable in different temperatures ranging from -70 °C to 25 °C, and acidic pH conditions in plasma for a month, when protected from light, but it was unstable in alkali pH and in lighting conditions.

Conclusions: In lighting conditions, most of t-Res was isomerized to the other isomer cis-resveratrol. t-Res is unstable in alkali pH and when exposed to light. t-Res was quite stable at other conditions, and several freeze-thaw cycles did not have any effect on its stability.

Keywords

Resveratrol

Stability

Antioxidants

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Author(s):

Milad Iranshahy:[PubMed][Scholar]
Amir Hooshang Mohammadpour:[PubMed][Scholar]
Mohammad Hassanzadeh-Khayyat:[PubMed][Scholar]
Mehrdad Iranshahi:[PubMed][Scholar]