Evaluation the effect of topical and systemic celecoxib on serum antioxidant in induction of tongue neoplasm in rat

  Background : Oral cancer is one of the ten most frequent cancers worldwide. Reactive oxygen species (ROS), may play a key role in human cancer development. Inflammation occurs in cancers and increases oxidative stress agent COX₂ over expression in oral premalignant lesions. Several studies showed COX₂ inhibitors con improve antioxidant system. The aim of this study is the evaluation the effect of topical and systemic celecoxib on serum antioxidant in induction of tongue neoplasm in rat.

  m aterials and Method : Fifty male Sprague Dawley adult 3-3.5 months' old rats were used as animal model in this study. The tongue SCC was induced by a daily administration of 30 ppm 4-nitroquinoline 1-oxide (4-NQO), in drinking water, for 8 months. The rats in case groups received dietary or topical CCB. CCB powder was added to powdered basal diet and e ach rat received CCB with daily food. Adhesive Gels were prepared by mixing the base plasty with oral paste including two different dose of CCB. These groups include: group A» 30 ppm 4-NQO treatment group B» 30 ppm 4-NQO+dietary CCB treatment group C» 30 ppm 4-NQO+topical low dose CCB treatment group D» 30 ppm 4NQO+high dose topical CCB treatment and group E» dietary CCB as control+water.

  Results : Statistical analysis showed significant differens between groups in total antioxidant, hemoglobin and nutrophilic count.  �

Conclusion : Topical CCB can use as adjuvant treatment for premalignant lesions