Archives of Clinical Infectious Diseases
Mohammed Varahram1, 
Parissa Farnia
1,*, Sabar Anoosheh1, Mehdi Kazampour1, Muayad Merza1, Shima Saeif1, Mohammad Reza Masjedi1, Ali Akbar Velayati1VitaminD receptor (VDR) and tumor necrosis factoralpha (TNF-?) genes are thought to be important in the intracellular killing of mycobacteria. This study aimed to determine the association of VDR and TNF-? variant with development of pulmonary tuberculosis (PTB) among Iranian patients.
Selected regions of VDR and TNF-? were amplified, and then the PCR products were digested using restriction enzyme (RFLP). Digested products were run on 8% polyacrylamide gel, and were stained with silver nitrate. Single nucleotide polymorphisms (SNPs) at restriction sites of BsmI, and FokI of VDR gene and SNPs of TNF-? at -238,-308, -244,-857,-863 positions were analyzed by PCR-RFLP among 117 PTB cases and 60 healthy controls.
No statistically significant difference was observed in allele frequencies of FokI of VDR and TNF-? at -238, -244,-863 and 857 position. Although, the frequency of b allele of BsmI (p=0.001) and -308 A variant in TNF-? promoter region (p=0.006) were significantly more in PTB patients than healthy controls. The frequency of extended diplotypes were different in patients and control subjects (p<0.05).
This study confirmed the association of VDR BsmI and TNF-? -308A with susceptibility to tuberculosis in Iranian PTB patients. In addition, the results showed the importance of haplotypes and diplotypes analysis in determining the host susceptibility against TB
© 2009, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
Metanat M, Narooie Nejad M, Salehi M, Moazen J, Sanei-moghaddam E, et al. Comparison of Genetic Polymorphisms of TNF-α and IL-10 Genes Between Tuberculosis Patients and Healthy Blood Donors. Arch Clin Infect Dis. 2013;8(3):e18270. doi: https://doi.org/10.5812/archcid.18270
Velayati AA, Farnia P, Farahbod AM, Afraei Karahrudi M, Derakhshaninezhad Z, et al. Association of Receptors, Purinergic P2X7 and Tumor Necrosis Factor-Alpha Gene Polymorphisms in Susceptibility to Tuberculosis Among Iranian Patients. Arch Clin Infect Dis. 2013;8(3):e16087. doi: https://doi.org/10.5812/archcid.16087
Taheri M, Naderi M, Hashemi M, Abiri M, Sarabandi M. Association Between IL12A rs568408, IL12B rs3212227 and IL-12 Receptor rs383483 Polymorphisms and Risk of Pulmonary Tuberculosis. Arch Clin Infect Dis. 2017;12(1):e39318. doi: https://doi.org/10.5812/archcid.39318
Naserpour Farivar T, Naderi M, Johari P. Frequency of NRAMP-1 Gene Polymorphism at INT4, D543 and 3'UTR loci in Tuberculosis patients and control group in Zahedan. J Inflamm Dis. 2024;17(4):e155790. doi:
Davoodi H, Allah. Ghaemi E, Jamali A, Naeeme JS, Shakeri F. Arg677Trp and Arg753Gln Polymorphisms in TLR2 Genes Detected in Patients With Tuberculosis in Golestan Province, Iran. Jundishapur J Microbiol. 2018;11(4):e13933. doi: https://doi.org/10.5812/jjm.13933
Ordering Reprints
Articles are published under the Creative Commons license stated on each article. No permission or royalty fee is required for uses permitted by that license. CCC handles optional bulk and customized reprint orders. Any quotation covers production and delivery services only, not copyright permission. > Request Reprints from CCC
Author(s):
