Archives of Clinical Infectious Diseases
Infectious Diseases and Tropical Medicine Research Center, SBUMS
Authors
1,*, Sabar Anoosheh1, Mehdi Kazampour1, Muayad Merza1, Shima Saeif1, Mohammad Reza Masjedi1, Ali Akbar Velayati1VitaminD receptor (VDR) and tumor necrosis factoralpha (TNF-?) genes are thought to be important in the intracellular killing of mycobacteria. This study aimed to determine the association of VDR and TNF-? variant with development of pulmonary tuberculosis (PTB) among Iranian patients.
Selected regions of VDR and TNF-? were amplified, and then the PCR products were digested using restriction enzyme (RFLP). Digested products were run on 8% polyacrylamide gel, and were stained with silver nitrate. Single nucleotide polymorphisms (SNPs) at restriction sites of BsmI, and FokI of VDR gene and SNPs of TNF-? at -238,-308, -244,-857,-863 positions were analyzed by PCR-RFLP among 117 PTB cases and 60 healthy controls.
No statistically significant difference was observed in allele frequencies of FokI of VDR and TNF-? at -238, -244,-863 and 857 position. Although, the frequency of b allele of BsmI (p=0.001) and -308 A variant in TNF-? promoter region (p=0.006) were significantly more in PTB patients than healthy controls. The frequency of extended diplotypes were different in patients and control subjects (p<0.05).
This study confirmed the association of VDR BsmI and TNF-? -308A with susceptibility to tuberculosis in Iranian PTB patients. In addition, the results showed the importance of haplotypes and diplotypes analysis in determining the host susceptibility against TB
© 2009, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
Metanat M, Narooie Nejad M, Salehi M, Moazen J, Sanei-moghaddam E, et al. Comparison of Genetic Polymorphisms of TNF-α and IL-10 Genes Between Tuberculosis Patients and Healthy Blood Donors. Arch Clin Infect Dis. 2013;8(3):e18270. doi: https://doi.org/10.5812/archcid.18270
Velayati AA, Farnia P, Farahbod AM, Afraei Karahrudi M, Derakhshaninezhad Z, et al. Association of Receptors, Purinergic P2X7 and Tumor Necrosis Factor-Alpha Gene Polymorphisms in Susceptibility to Tuberculosis Among Iranian Patients. Arch Clin Infect Dis. 2013;8(3):e16087. doi: https://doi.org/10.5812/archcid.16087
Taheri M, Naderi M, Hashemi M, Abiri M, Sarabandi M. Association Between IL12A rs568408, IL12B rs3212227 and IL-12 Receptor rs383483 Polymorphisms and Risk of Pulmonary Tuberculosis. Arch Clin Infect Dis. 2017;12(1):e39318. doi: https://doi.org/10.5812/archcid.39318
Naserpour Farivar T, Naderi M, Johari P. Frequency of NRAMP-1 Gene Polymorphism at INT4, D543 and 3'UTR loci in Tuberculosis patients and control group in Zahedan. J Inflamm Dis. 2024;17(4):e155790. doi:
Davoodi H, Allah. Ghaemi E, Jamali A, Naeeme JS, Shakeri F. Arg677Trp and Arg753Gln Polymorphisms in TLR2 Genes Detected in Patients With Tuberculosis in Golestan Province, Iran. Jundishapur J Microbiol. 2018;11(4):e13933. doi: https://doi.org/10.5812/jjm.13933
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