Determination of heavy metals and anthraquinone in black tea

Author(s):
Narges ChamkouriNarges Chamkouri1,*, Sanaz KarimpourSanaz Karimpour2, jamileh derisjamileh deris2, Zahra KoolivandZahra KoolivandZahra Koolivand ORCID3, Seyed Mohammad Ali MalaekehSeyed Mohammad Ali Malaekeh2, Fariba DerisFariba Deris2
1Department of Basic Sciences, Faculty of Medicine, Abadan University of Medical Sciences, Abadan, Iran
2Abadan University of Medical Sciences, Abadan, Iran
3Department of Laboratory Sciences, School of Allied Medical Sciences, Abadan University of Medical Sciences, Abadan, Iran
*Corresponding Author: Department of Basic Sciences, Faculty of Medicine, Abadan University of Medical Sciences, Abadan, Iran. Email:[email protected]

Comprehensive Health and Biomedical Studies:Vol. 1, issue 1; 36-44
Published online:Jul 01, 2022
Article type:Research Article
Received:Oct 10, 2021
Accepted:Jan 15, 2022
How to Cite:Chamkouri N, Karimpour S, deris J, Koolivand Z, Malaekeh SMA, et al. Determination of heavy metals and anthraquinone in black tea. Compr Health Biomed Stud. 2022;1(1):e142859. doi: https://doi.org/10.22034/hmrj.2022.143241

Abstract

Introduction: A rapid and simple method was investigated for determining the concentration of some contaminants including heavy metals such as lead (Pb), cadmium(Cd), chromium (Cr), and anthraquinone in black tea samples in Iran. 

Methods: Heavy metals such as Pb, Cd, and Cr in tea samples were detected using inductively coupled plasma optical emission spectrometry (ICP-OES) after microwave acid digestion (MAD). The anthraquinone in infusion tea samples was determined via liquid chromatography–photodiode array detector after the dispersive liquid-liquid microextraction method (DLLME). 

Results: Recoveries of all the tea samples in heavy metals and anthraquinone were in the range of 75% - 89% and 71%-96, respectively. The results of recoveries are in the range of the Association of Official Analytical Chemists (AOAC) and the European Union. RSD in the MAD and DLLME methods was less than 5%. The mean concentrations of Pb, Cd, Cr, and anthraquinone were 0.724, 0.021, 2.98 ppm, and 2.27 ppb. 

Conclusion: Overall our results showed that MAD and DLLME methods could successfully determine the concentration of some contaminants such as Pb, Cd, Cr, and anthraquinone in black tea samples.

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The Author(s) 2021. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. The Creative Commons Public Domain Dedication waiver (http://creativeco mmons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated in a credit line to the data.

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