Fulltext
The Full text is available in PDF.
International Journal of Cancer Management
The Official Journal of Cancer Research Center (CRC), Shahid Beheshti University of Medical Sciences
e-ISSN 2538-497X
p-ISSN 2538-4422
The Study of DNA Methylation of bax Gene Promoter in Breast and Colorectal Carcinoma Cell Lines
Authors
Mohsen Alipour1, Seyed Jalal Zargar1,*, Shahrokh Safarian1, Shamileh Fouladdel2, Ebrahim Azizi3, Naser Jafargholizadeh1
1Dept. of Cell and Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran
2Dept. of Pharmacology and Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
3Dept. of Pharmacology and Toxicology, Faculty of Pharmacy, Tehran University of Medical Sciences, Biotechnology Research Center, Tehran University of Medical Sciences, Tehran, Iran
*Corresponding Author: Corresponding author: Seyed Jalal Zargar, Dept. of Cell and Molecular Biology, School of Biology, College of Science, University of Tehran, Tehran, Iran, Tel: +98-2161113646, E-mail: Email: zargar@khayam.ut.ac.ir
Published online:Jun 30, 2013
Article type:Research Article
Received:Nov 20, 2012
Accepted:Feb 18, 2013
How to Cite:Alipour M, Zargar SJ, Safarian S, Fouladdel S, Azizi E, et al. The Study of DNA Methylation of bax Gene Promoter in Breast and Colorectal Carcinoma Cell Lines. Int J Cancer Manag. 2013;6(2):e80399. doi:
Abstract
Background: The Bcl-2 protein family members have known as essential controllers of mitochondrial pathway of apoptosis. Bax is a proapoptotic member of Bcl-2 protein family, which is well known to play crucial roles for apoptosis control. bax has been implicated as potential tumor suppressor in certain solid tumors such as breast and colorectal carcinoma. DNA methylation of promoter associated CpG islands has known as a common mechanism for gene inactivation in tumor cells.
Methods: The Methylation Specific PCR (MSP) has used to find the methylation profile of the bax gene promoter CpG islands in colorectal and breast cancer cell lines.
Results: We have not detected any kind of "CpG islands hypermethylation" in promoter region of the bax gene in T47D, MCF7 (as ER positive), MDA-MB-231 and MDA-MB-468 (as ER negative) breast carcinoma-derived cell lines and colorectal cancer cell lines H29 and Caco II.
Conclusion: It seems that CpG island methylation could not play the main role in down-regulation of bax gene in breast and colon cancers.
Highlights
Copyright
© 2013, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
Similar Articles
23
Dec
2019
The Relationship Between Promoter Methylation of RASSF1A Gene and Prognosis of Patients Affected with Breast Cancer in Khuzestan Province
Javad Mohammadi Asl,
Farideh Ghanbari Mardasi,
Ghasem Saki,
Fakher Rahim,
Iran Rashidi,
Nastaran Ranjbari
,et al.Mohammadi Asl J, Ghanbari Mardasi F, Saki G, Rahim F, Rashidi I, et al. The Relationship Between Promoter Methylation of RASSF1A Gene and Prognosis of Patients Affected with Breast Cancer in Khuzestan Province. Gene Cell Tissue. 2019;7(1):e96452. doi: https://doi.org/10.5812/gct.96452
19
Jun
2009
Methylation of the GSTP1 gene promotor in colorectal cancer
Hamid Nomani,
Naghmeh Jalejo,
Bahram Yaghmaei,
Shahrokh Mohammadzadeh Ghobadlu,
AliAsghar Keshavarz
Nomani H, Jalejo N, Yaghmaei B, Mohammadzadeh Ghobadlu S, Keshavarz A. Methylation of the GSTP1 gene promotor in colorectal cancer. J Kermanshah Univ Med Sci. 2009;13(1):e79827. doi:
1
Apr
2015
Epigenetic BRCA2 Gene in Epithelial Ovarian Cancer
Fayazeh Sarjooghian,
Kahin Shahanipour,
Ahmad Shabanizadeh
Sarjooghian F, Shahanipour K, Shabanizadeh A. Epigenetic BRCA2 Gene in Epithelial Ovarian Cancer. Gene Cell Tissue. 2015;2(2):e28416. doi: https://doi.org/10.17795/gct-28416
19
Jan
2025
Investigation of the Methylation Status of Two Autophagy Genes, LC3B and ATG5, with Autophagy Gene Polymorphism ATG16L1 in Patients with Colorectal Cancer in Fars Province
Leila Shafiee,
Pooneh Mokarram,
Seyedeh Azra Shamsdin,
Zahra Khoshdel
Shafiee L, Mokarram P, Shamsdin SA, Khoshdel Z. Investigation of the Methylation Status of Two Autophagy Genes, LC3B and ATG5, with Autophagy Gene Polymorphism ATG16L1 in Patients with Colorectal Cancer in Fars Province. Shiraz E-Med J. 2025;26(4):e142779. doi: https://doi.org/10.5812/semj-142779
30
Apr
2017
Study of the Role of siRNA Mediated Promoter Methylation in DNMT3B Knockdown and Alteration of Promoter Methylation of CDH1, GSTP1 Genes in MDA-MB -453 Cell Line
Mojgan Naghitorabi,
Hamid Mir Mohammad Sadeghi,
Javad Mohammadi Asl,
Mohammad Rabbani,
Abbas Jafarian-Dehkordi
Naghitorabi M, Mir Mohammad Sadeghi H, Mohammadi Asl J, Rabbani M, Jafarian-Dehkordi A. Study of the Role of siRNA Mediated Promoter Methylation in DNMT3B Knockdown and Alteration of Promoter Methylation of CDH1, GSTP1 Genes in MDA-MB -453 Cell Line. Iran J Pharm Res. 2017;16(2):e124993. doi: https://doi.org/10.22037/ijpr.2017.2042
Cited by
- Scopus by DOI: 0
Last Update: 1 week ago
- Scopus by Title: 12
Last Update: 1 week ago
- Scopus by Title (Ref): 12
Last Update: 1 week ago
- CrossRef: 0
Last Update: 3 hours ago
- OpenAlex: 0
Last Update: 7 hours ago
- OpenCitations: 0
Last Update: 5 days ago
Comments
Number of Comments:0