Cell Survival Entropy and Cellular Resistance Activation Dose: Effect of Calprotectin on Gastric Adenocarcinoma Cell Line

Background: The survival curves generally have been used for analysis of cell survival under stress conditions. They depict the relationship between the fraction of cells retaining their reproductive integrity and the absorbed dose. The median lethal dose, Lethal Concentration, 50% (LC50) of a toxin, radiation, or pathogen is the dose required to kill half the members of a tested population after specified test duration. LC50 frequently are used as a general indicator of a substance's acute toxicity. In this study a novel thermodynamic model is introduced to examine cell survival under stress condition. The survival function describes the surviving fraction of cell survival in different doses and contains information about cell survival. However, for interpretation of cells behavior under stress condition, thermodynamic formalism is utilized.

Methods: Gastric adenocarcinoma cells line were seeded, in the 96 well plates and incubated at 37 °C under 5% CO2 atmosphere for 48 hours. After that, the various concentrations of calprotectin were induced into cells for 48hours for MTT assay proposes. Viability data were analyzed by entropy function and correspond results was plotted. The statistical analysis applied for data validation.

Results: The entropy of survival function has a maximum value at LC50 and is asymmetric around LC50 value, and the rate of change of entropy function is different below and above LC50 value which indicates that cells have different behavior. The entropy function has a more gentle slope above LC50 so we can assume that living cells above LC50 are more resistant to poison used.

Conclusion: It can be concluded that regard entropy function and its derivations provides more possibility for revealing mechanism of cell behavior in stress conditions.