Production of dark neurons in the hippocampal CA1, CA2, and dentate gyrus in adult rats after repeated sevoflurane exposure in pre-adolescence. A, Light-microscopic appearance of toluidine blue-stained dark neurons in 10-μm hippocampus sections (CA1, CA2, and DG areas); B, Dark neuron numbers in CA1 and CA2 regions of the sevoflurane groups were significantly higher than in the control groups (P < 0.001 male sevoflurane vs. male control in CA1 and CA2 (#P < 0.05 female sevoflurane vs. female control in CA1 and CA2; P < 0.05 male sevoflurane vs. male control in DG region). All graphs are presented as mean ± SEM.
Figure 7.
