Isolation of Enteropathogenic Escherichia coli (EPEC) From Raw Milk in Kermanshah by Polymerase Chain Reaction (PCR)

Author(s):
Pantea MohammadiPantea Mohammadi1, Ramin AbiriRamin AbiriRamin Abiri ORCID2,*
1Medical biology Research Center, University of Medical Sciences, Kermanshah, IR Iran
2Department of Microbiology, Faculty of Medicine, University of Medical Sciences, Kermanshah, IR Iran
*Corresponding Author: Department of Microbiology, Faculty of Medicine, University of Medical Sciences, Parastar Blvd., Kermanshah, IR Iran. Tel: +98-9122773648, Fax: +98-8314276471, [email protected]. Email: [email protected]

Jundishapur Journal of Microbiology:Vol. 6, issue 4; 5439
Published online:Jun 09, 2013
Article type:Research Article
Received:May 04, 2012
Accepted:Aug 07, 2012
How to Cite:Mohammadi P, Abiri R. Isolation of Enteropathogenic Escherichia coli (EPEC) From Raw Milk in Kermanshah by Polymerase Chain Reaction (PCR). Jundishapur J Microbiol. 2013;6(4):5439. doi: https://doi.org/10.5812/jjm.5439

Abstract

Background:

Infectious diarrhoeal diseases cause major problems throughout the world and are responsible for considerable morbidity and mortality. Enteropathogenic Escherichia coli (EPEC) may cause infantile diarrhoea among children in developing countries.

Objectives:

The aim of this study was to define the prevalence of EPEC strains in raw milk samples.

Materials and Methods:

Raw milk samples collected from various cow farms in Kermanshah, Iran, during the period of 22nd June to 22nd September 2009 and were examined for EPEC presence using PCR reactions targeting eaeA, and then stx1 and stx2.

Results:

Of the 206 samples, 17 (8.25%) were contaminated with E. coli eaeA positive and stx1 and stx2 negative (EPEC).

Conclusions:

Our results confirm that raw milk recovered in Kermanshah may be a source for gastrointestinal infections by EPEC and strict preventive measures should be adopted to decrease contamination of milk with EPEC and other bacteria originated from animals.

Full Text

Full text is available in PDF

Copyright

© 2013, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.

Similar Articles

25
Jul
2018
Comparison of Coliform Contamination and Endotoxin Levels in Raw Cow’s Milk

Comparison of Coliform Contamination and Endotoxin Levels in Raw Cow’s Milk

Fatemeh Afshari Asfidoajani,
Maryam Mohammadi Sichani

Afshari Asfidoajani F, Mohammadi Sichani M. Comparison of Coliform Contamination and Endotoxin Levels in Raw Cow’s Milk. Zahedan J Res Med Sci. 2018;20(5):e59333. doi: https://doi.org/10.5812/zjrms.59333

26
Apr
2023
Gene Cell Tissue

Frequency of Cytolethal Distending Toxin Genes in Escherichia coli Isolates from Bovine Mastitis in Tabriz City in 2020

Saman Mahdavi,
Masoumeh Salimi,
Jafar Rahmani Kahnamoei

Mahdavi S, Salimi M, Rahmani Kahnamoei J. Frequency of Cytolethal Distending Toxin Genes in Escherichia coli Isolates from Bovine Mastitis in Tabriz City in 2020. Gene Cell Tissue. 2023;10(2):e132228. doi: https://doi.org/10.5812/gct-132228

7
May
2018
Virulence Genes and Phenotypic Evaluation of the Antibiotic Resistance of Vero Toxin Producing <i>Escherichia coli</i> Recovered From Milk, Meat, and Vegetables

Virulence Genes and Phenotypic Evaluation of the Antibiotic Resistance of Vero Toxin Producing Escherichia coli Recovered From Milk, Meat, and Vegetables

Zohreh Mashak

Mashak Z. Virulence Genes and Phenotypic Evaluation of the Antibiotic Resistance of Vero Toxin Producing Escherichia coli Recovered From Milk, Meat, and Vegetables. Jundishapur J Microbiol. 2018;11(5):e62288. doi: https://doi.org/10.5812/jjm.62288

1
Jan
2011

The Spatial Distribution of Bacteria Pathogens in Raw Milk Consumption on Malayer City, Iran

M Pourhassan,
Alireza Taravat Najafabadi

Pourhassan M, Taravat Najafabadi A. The Spatial Distribution of Bacteria Pathogens in Raw Milk Consumption on Malayer City, Iran. Shiraz E-Med J. 2011;12(1):e77036. doi:

1
Oct
2013

Prevalence of Escherichia coli O157:H7, Listeria monocytogenes and Salmonella spp. in Beef, Buffalo and Lamb Using Multiplex PCR

Mehdi Zarei,
Narjes Basiri,
Amirhesam Jamnejad,
Mohammad Hadi Eskandari

Zarei M, Basiri N, Jamnejad A, Eskandari MH. Prevalence of Escherichia coli O157:H7, Listeria monocytogenes and Salmonella spp. in Beef, Buffalo and Lamb Using Multiplex PCR. Jundishapur J Microbiol. 2013;6(8):e7244. doi: https://doi.org/10.5812/jjm.7244

Download PDF483.09 KB

Crossmark

Crossmark

Checking

Share on
Cited by
Metrics

Ordering Reprints

Articles are published under the Creative Commons license stated on each article. No permission or royalty fee is required for uses permitted by that license. CCC handles optional bulk and customized reprint orders. Any quotation covers production and delivery services only, not copyright permission. > Request Reprints from CCC