Journal of Kermanshah University of Medical Sciences
Peer-reviewed Medical Quarterly
Outlines
Identification of Acinetobacter baumannii via amplified ribosomal DNA restriction analysis
Author(s):
1Dept. of Biology, School of Natural Sciences, University of Tabriz, Tabriz, Iran
2Dept. of Microbiology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
*Corresponding Author: Corresponding author: Gholamreza Zarrini, Dept. of Biology, School of Natural Sciences, University of Tabriz, Tabriz, Iran, Tel: +98 411 3392707 Email: [email protected]
Published online:Feb 27, 2014
Article type:Research Article
Received:Sep 09, 2013
Accepted:Dec 03, 2013
How to Cite:Rezaei D, Zarrini G, Ahangarzadeh Rezaei M. Identification of Acinetobacter baumannii via amplified ribosomal DNA restriction analysis. J Kermanshah Univ Med Sci. 2014;17(11):e74308. doi: https://doi.org/10.22110/jkums.v17i11.1360
Abstract
Background: Acinetobacter baumannii is a multi-resistant opportunistic nosocomial pathogen responsible for hospital outbreaks worldwide. In addition to common microscopic and biochemical methods, the Amplified Ribosomal DNA Restriction Analysis (ARDRA) was tested to identify Acinetobacter genomic species (DNA groups).
Methods: In this study, standard biochemical tests were used for identification of isolates. The genomic species of Acinetobacter spp. was confirmed by Amplified Ribosomal DNA Restriction Analysis (ARDRA). PCR products of 16S rRNA were digested with AluI, MboI and HhaI restriction enzymes.
Results: ARDRA proved to be a rapid and reliable method for identification of Acinetobacter baumannii (genome species 2) of the Acinetobacter genomic species, including the closely related genomic species (genomic species 1 (A. calcoaceticus), 2 (A. baumannii), 3, and TU13).
Conclusion: The results of this study suggested that ARDRA with AluI, MboI and HhaI restriction enzymes can be used for identificationof A. baumannii which may help to elucidate the ecology and clinical significance of different species of this genus. Since ARDRA is performed by universal primers of 16S rDNA gene, it is expected to be applicable to identifying most bacterial species.
References
- 1.The references of this article is available on PDF.
Copyright
© 2014, Journal of Kermanshah University of Medical Sciences. This open-access article is available under the Creative Commons Attribution-NonCommercial 4.0 (CC BY-NC 4.0) International License (https://creativecommons.org/licenses/by-nc/4.0/), which allows for the copying and redistribution of the material only for noncommercial purposes, provided that the original work is properly cited.
Similar Articles
20
Feb
2015
Antimicrobial Susceptibility and Clonal Relation Between Acinetobacter baumannii Strains at a Tertiary Care Center in Turkey
Gulfem Ece,
Bayri Erac,
Hasan Yurday Cetin,
Cem Ece,
Aysegul Baysak
Ece G, Erac B, Yurday Cetin H, Ece C, Baysak A. Antimicrobial Susceptibility and Clonal Relation Between Acinetobacter baumannii Strains at a Tertiary Care Center in Turkey. Jundishapur J Microbiol. 2015;8(2):e15612. doi: https://doi.org/10.5812/jjm.15612
13
Feb
2016
Characterization of Oxacillinase and Metallo-β-Lactamas Genes and Molecular Typing of Clinical Isolates of Acinetobacter baumannii in Ahvaz, South-West of Iran
Saeed Shoja,
Mojtaba Moosavian,
Soodabeh Rostami,
Fariba Abbasi,
Mohammad Amin Tabatabaiefar,
Amir Peymani
Shoja S, Moosavian M, Rostami S, Abbasi F, Tabatabaiefar MA, et al. Characterization of Oxacillinase and Metallo-β-Lactamas Genes and Molecular Typing of Clinical Isolates of Acinetobacter baumannii in Ahvaz, South-West of Iran. Jundishapur J Microbiol. 2016;9(5):e32388. doi: https://doi.org/10.5812/jjm.32388
31
May
2017
Clinical Isolates of Acinetobacter baumannii From Tehran Hospitals: Pulsed-field Gel Electrophoresis Characterization, Clonal Lineages, Antibiotic Susceptibility, and Biofilm-forming Ability
Mahdi Akbari Dehbalaei,
Shahin Najar-Peerayeh,
Morovat Taherikalani,
Mehrdad Behmanesh
Akbari Dehbalaei M, Najar-Peerayeh S, Taherikalani M, Behmanesh M. Clinical Isolates of Acinetobacter baumannii From Tehran Hospitals: Pulsed-field Gel Electrophoresis Characterization, Clonal Lineages, Antibiotic Susceptibility, and Biofilm-forming Ability. Jundishapur J Microbiol. 2017;10(7):e13790. doi: https://doi.org/10.5812/jjm.13790
20
Jun
2015
Phenotypic and Molecular Epidemiology of Acinetobactercalcoaceticusbaumannii Complex Strains Spread at Nemazee Hospital of Shiraz, Iran
Mojtaba Anvarinejad,
Aziz Japoni,
Mohammad Ali Davarpanah,
Hossein Mahmudi,
Caterina Mammina,
Afsaneh Vazin
Anvarinejad M, Japoni A, Davarpanah MA, Mahmudi H, Mammina C, et al. Phenotypic and Molecular Epidemiology of Acinetobactercalcoaceticusbaumannii Complex Strains Spread at Nemazee Hospital of Shiraz, Iran. Jundishapur J Microbiol. 2015;8(6):e19180. doi: https://doi.org/10.5812/jjm.8(5)2015.19180
6
Mar
2019
Evaluation of Multilocus Variable-Number Tandem-Repeat (MLVA-8Orsay) for Typing of Carbapenem-Resistant Acinetobacter baumannii Isolated from Patients in Tehran, Iran
Shahin Najar-Peerayeh,
Afsaneh Karmostaji
Najar-Peerayeh S, Karmostaji A. Evaluation of Multilocus Variable-Number Tandem-Repeat (MLVA-8Orsay) for Typing of Carbapenem-Resistant Acinetobacter baumannii Isolated from Patients in Tehran, Iran. Arch Clin Infect Dis. 2019;14(1):e64402. doi: https://doi.org/10.5812/archcid.64402
Download PDF255.72 KB
Crossmark
Checking
Comments
Number of Comments:0Cited by
- Scopus by DOI: 0
Last Update: 1 week ago
- Scopus by Title: 0
Last Update: 1 week ago
- Scopus by Title (Ref): 1
Last Update: 1 week ago
- CrossRef: 0
Last Update: 5 days ago
Ordering Reprints
Articles are published under the Creative Commons license stated on each article. No permission or royalty fee is required for uses permitted by that license. CCC handles optional bulk and customized reprint orders. Any quotation covers production and delivery services only, not copyright permission. > Request Reprints from CCC
Search Relations
Author(s):
Related Articles