Journal of Kermanshah University of Medical Sciences
Peer-reviewed Medical Quarterly
e-ISSN 2588-2570
p-ISSN 2588-2562
Image Credit:J Kermanshah Univ Med Sci
Outline
Identification of Acinetobacter baumannii via amplified ribosomal DNA restriction analysis
Authors
1Dept. of Biology, School of Natural Sciences, University of Tabriz, Tabriz, Iran
2Dept. of Microbiology, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
*Corresponding Author: Corresponding author: Gholamreza Zarrini, Dept. of Biology, School of Natural Sciences, University of Tabriz, Tabriz, Iran, Tel: +98 411 3392707 Email: zarrini@tabrizu.ac.ir
Published online:Feb 27, 2014
Article type:Research Article
Received:Sep 09, 2013
Accepted:Dec 03, 2013
How to Cite:Rezaei D, Zarrini G, Ahangarzadeh Rezaei M. Identification of Acinetobacter baumannii via amplified ribosomal DNA restriction analysis. J Kermanshah Univ Med Sci. 2014;17(11):e74308. doi: https://doi.org/10.22110/jkums.v17i11.1360
Abstract
Background: Acinetobacter baumannii is a multi-resistant opportunistic nosocomial pathogen responsible for hospital outbreaks worldwide. In addition to common microscopic and biochemical methods, the Amplified Ribosomal DNA Restriction Analysis (ARDRA) was tested to identify Acinetobacter genomic species (DNA groups).
Methods: In this study, standard biochemical tests were used for identification of isolates. The genomic species of Acinetobacter spp. was confirmed by Amplified Ribosomal DNA Restriction Analysis (ARDRA). PCR products of 16S rRNA were digested with AluI, MboI and HhaI restriction enzymes.
Results: ARDRA proved to be a rapid and reliable method for identification of Acinetobacter baumannii (genome species 2) of the Acinetobacter genomic species, including the closely related genomic species (genomic species 1 (A. calcoaceticus), 2 (A. baumannii), 3, and TU13).
Conclusion: The results of this study suggested that ARDRA with AluI, MboI and HhaI restriction enzymes can be used for identificationof A. baumannii which may help to elucidate the ecology and clinical significance of different species of this genus. Since ARDRA is performed by universal primers of 16S rDNA gene, it is expected to be applicable to identifying most bacterial species.
Highlights
References
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Copyright
© 2014, Journal of Kermanshah University of Medical Sciences. This open-access article is available under the Creative Commons Attribution-NonCommercial 4.0 (CC BY-NC 4.0) International License (https://creativecommons.org/licenses/by-nc/4.0/), which allows for the copying and redistribution of the material only for noncommercial purposes, provided that the original work is properly cited.
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