Identification and cloning of oxidoreductase gene from Rhodococcus erythropolis in E.Coli

Introduction: The combustion of fossil fuels leads to release of sulfur oxide (SO2) and therefore is a source of environmental pollution. Biodesulfurization could be an alternative to remove sulfur compounds from fossil fuels. Previously three genes (dszA, B and C) encoding the 4S pathway in Biodesulfurization were identified from a newly isolated Rhodococcus, IGTS8. Material and Methods: The DNA sequence of a 4kb gene cluster that carries the above genes for this pathway was determined. Results: The three genes encoding the 4S pathway were cloned under control of the promoter in P.aerogimusa ATCC 9027, and biodesulfurization of DBT in recombinal strain enhanced compared to the native bacteria R.IGTS8. The previous studies which have been purified from the IGTS8 strain needs reducing equivalents to support the three oxigenation steps. DszD which has been purified from the IGTS8 stain was postulated to be the FMN-NADH reductase the supplies the monooxygenases dszD and dszA with the necessary free reduced flavin (FMNH2). Conclusion: The DszD oxidureductase is encoded by the dszD gene in 4S pathway. Isolation, cloning and overexpression of the dszD gene is important to increase the biodesulfurization in vivo. In this project, Identification, isolationcloning and expression of dszD gene from Rhodococcus erythropolis in E.Coli will be investigated.