Development of Agglutination Test for Detection of isolated Mannoprotein Antigen from Candida Albicans

authors:

avatar Masoumeh Rajabibazl 1 , avatar Mohammad Javad Rasaee 2 , avatar Mahtab Nouri Fard 3 , avatar Zohreh Farahnezhad 4 , *

Assistant Professor, Clinical Biochemistry Department, Faculty of Medicine, Shahid Beheshti University of Medicine, Tehran, Iran, Andorra
Professor, Medical Biotechnology Department, Medical Sconces Faculty, Tarbiat Modares University, Tehran, Iran, Andorra
- Assistant Professor, Infectious Disease Department, Faculty of Medicine, AJA University of Medical Sconces, Tehran, Iran, Andorra
Assistant Professor, Mycology Department, Faculty of Medicine, AJA University of Medical Sconces, Tehran, Iran, Andorra
Annals of Military and Health Sciences Research: Vol.11, issue 2; e65198
published online: May 11, 2013
article type: Original Article
received: December 26, 2012
accepted: April 11, 2013

how to cite: Rajabibazl M , Rasaee M J, Nouri Fard M , Farahnezhad Z. Development of Agglutination Test for Detection of isolated Mannoprotein Antigen from Candida Albicans. Ann Mil Health Sci Res. 2013;11(2):e65198. 

Abstract

Background: Candida albicans is one of the most common causes of hospital infections. The aim of this study was to develop a rapid, easy and sensitive way to detect candida albicans. Material and Methods: Polyclonal antibodies were prepared in rabbits. The antibodies were purified by salt concentration and Ion exchange chromatography. The purified antibody was coated onto the colloidal gold. Results: Color change (red to blue) was observed when the purified antigens (mannoprotein of Candida albicans cell wall) were added to Polyclonal antibodies. The method was sensitive and easy. Conclusion: This study indicated that using colloidal gold particle agglutination method can be used for accurate and rapid candida detection Method.

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