Fauna and Seasonal Abundance of Domestic Mites as Respiratory Allergen in Three Military Areas, North of Iran in 2017 - 2018

authors:

avatar Abdolazim Mortazavi Lahijani 1 , avatar Ramin Khaghani 1 , * , avatar Hamidreza Hajiqanbar 2 , avatar Seyed Shahram Mirzamani 3

Department of Medical Parasitology, School of Medicine, AJA University of Medical Sciences, Tehran, Iran
Department of Entomology, Faculty of Agriculture, Tarbiat Modares University, Tehran, Iran
Health and Aid and Treatment Department, Iranian Naval Directorate of Health, Tehran, Iran
Annals of Military and Health Sciences Research: Vol.17, issue 2; e92403
published online: June 10, 2019
article type: Research Article
received: April 17, 2019
accepted: May 22, 2019
DOI: https://doi.org/10.5812/amh.92403

how to cite: Mortazavi Lahijani A, Khaghani R, Hajiqanbar H, Mirzamani S S. Fauna and Seasonal Abundance of Domestic Mites as Respiratory Allergen in Three Military Areas, North of Iran in 2017 - 2018. Ann Mil Health Sci Res. 2019;17(2):e92403. https://doi.org/10.5812/amh.92403.

Abstract

Background:

Domestic mites (DM) refer to all indoor mite species such as house dust mites (HDM) and some of the storage mites that are able to induce IgE-mediated sensitization. These microscopic mites are one of the most known risk factors of allergies and respiratory diseases in homes and other indoor places.

Objectives:

Considering the importance of DMs and lack of investigation on DMs in military areas of Iran, this study was designed to determine the fauna and biodiversity of DM in three military areas, North of Iran in 2017 - 2018.

Methods:

It was a cross-sectional study. Each military area was divided into three sampling zones (lieutenant dormitory, soldier dormitory and administrative place). In each sampling zone, seven places were randomly selected and dust samples were collected using a vacuum cleaner for 2 minutes from 1 m2 of the surface. After collection, samples were weighted separately. The mites were counted by a Stereo microscope, mounted in Hoyer’s medium, and identified by standard keys. The data were analyzed using statistical SPSS software version 17 and Microsoft Excel software version 2013 software.

Results:

A total of 648 dust samples were examined, in total 1043 mites were collected and identified that 741 mites were from six species of DM. The most abundant species were Dermatophagoides farinae (72.74%) and D. pteronyssinus (14.58%), and the less abundant species were Glycyphagus domesticus (0.94%) and Euroglyphusmaynei (0.54%). The most of DMs were found in the lieutenant dormitory (84.3%) and just 1.9% of DMs in the administrative place. The DMs were found almost throughout the year with the highest peaks in Jun. (11.1%), August (13.1%), September (14.7%), and October (11.1%), and the smallest peak in January (3.8%), February (4.2%), and March (3.2%).

Conclusions:

The results of this study show that all military areas are contaminated by DMs. Although the density of DM did not exceed the risk thresholds (10 mg mites/gram dust or 100 mites/gram dust), in the first and third lieutenant dormitories were near the risk threshold from January to November. Therefore, we suggest the preventive measures, particularly in the lieutenant dormitories, should be more effective than the past.

1. Background

Domestic mites (DM) refer to all indoor mite species that are able to induce IgE-mediated sensitization. The domestic mites consist of the house dust mites (HDM) and some of the storage mites (1, 2). In the last decades, the prevalence of allergic disease such as asthma has increased worldwide (3). Domestic mites are one of the major sources of indoor allergens that induce allergic diseases such as asthma and other allergic disorders. The importance of mites’ allergens in asthma and allergy have been confirmed by different studies (4, 5). During the digestion of food in different regions of the mites’ digestive tract, the cells of Midgut walls produce large quantities of digestion enzymes and a peritrophic membrane that protects the delicate hindgut from the damage by abrasion mix with the fecal pellets of dust mites that can trigger an immune response when humans are exposed to them (5-7). Public buildings and homes provide a good habitat for the DMs as well as humans (8). Mite allergens can be found in many areas of the homes such as carpets, mattress, furniture, and closet. The beds are the ideal habitats for the mites since the beds prepare the food, ideal temperature, and humidity for their propagation. The mite allergens may stock deep inside old pillows, mattresses, and carpets (9).

The pyroglyphid mites have been the object of most works on mite allergens (1, 2, 5, 7, 8, 10-12). Despite the fact that the principal allergen-producing mite species belong to the family Pyroglyphidae, some of the storage mites such as Blomia tropicalis are capable of inducing allergy as well (5). Based on recent surveys of allergen mites in Iran, the most common and abundant species are Dermatophagoides pteronyssinus, D. farinae, Blomia tropicalis, and Cheyletuseruditus (5, 13-16). Until now, only thirteen species of DMs have been recorded from homes, hotels, inns and kindergartens of Iran as follows: Dermatophagoides pteronyssinus, D. farinae, D. evansi, Euroglyphusmaynei, Acarussiro, Tyrophagusputrescentiae, Lepidoglyphus destructor, Glycyphagusfusca, G. domesticus, Cheyletusmalaccensis, Cheyletuseruditus and Cheyletusarcuatus (5, 13-20).

2. Objectives

Considering the importance of DM and lack of investigation on DM in military bases of Iran, this study was designed to determine the fauna and seasonal abundance of DMs in three northern military areas, Iran.

3. Methods

This cross-sectional study was carried out in three military areas, North of Iran, between September 2017 and August 2018.

Each military area was divided into three sampling zones (lieutenant dormitory, soldier dormitory, and administrative place). In the lieutenant dormitory and the soldier dormitory sampling zones, 7 places were randomly selected (four beds and three parts of the floor) and four places in the administrative place (two chairs and two parts of the floor). Dust samples were collected using a vacuum cleaner from 1 m2 of each sampling place in 2 minutes. The sampling has been done monthly. After collection, samples were placed in 120 milliliters vials, labeled separately, and transferred to the laboratory. In the laboratory, the samples were weighted separately, the mites were counted by a Stereo microscope, cleared in lactophenol solutions, mounted in Hoyer’s medium, and identified by standard keys. The data were analyzed using statistical SPSS version 17 and Microsoft Excel version 2013 software.

4. Results

A total of 648 dust samples were collected from three military areas, 1043 mites were collected and identified at the species level that 741 of them belonged to DMs. The collected DMs were identified as six species, five genera, and four families, including Dermatophagoides farinae, D. pteronyssinus, Euroglyphusmaynei (Pyroglyphidae), Blomia tropicalis (Echimyopodidae), Glycyphagusdomesticus (Glycyphagidae), and Cheyletusmalaccensis (Cheyletidae).

Dermatophagoides farina with 72.74% was the dominant species and present in all mite-positive samples. D. pteronyssinus and B. tropicalis with 14.57% and 7.02% were the second and third most frequent species, respectively (Table 1). E. maynei and G. domesticus with 0.54% and 0.94%, respectively were the least frequent species. Ch. malaccensis with 4.18% was the moderate frequent species. A total of four families were collected, Pyroglyphidae with 94.33 was the main and most frequent family. The prevalence of DMs in the second military area was less than other military areas and the prevalence of DMs in the first military area was a little more than third one (Table 1).

Table 1.

Mite Species Present in Dust Samples Collected from Three Military Areas, During 2017 - 2018a

SpeciesSpeciesTotal
First AreaSecond AreaThird Area
D. farinae235 (43.59)117 (21.7)187 (34.69)539 (72.74)
D. pteronyssinus27 (25)36 (33.33)45 (41.66)108 (14.57)
B. tropicalis23 (36.54)12 (23.08)17 (40.38)52 (7.02)
Ch. Malaccensis11 (35.48)13 (41.94)7 (22.58)31 (4.18)
G. domesticus5 (71.43)02 (28.57)7 (0.94)
E. maynei1 (25)03 (75)4 (0.54)
Total302 (40.76)178 (24.02)261 (35.22)741 (100)

aValues are expressed as No. (%).

In total, the DMs were found almost throughout the year, the highest abundance of DMs was in summer (37.4%) and the lowest in winter (11.2%). The highest peaks were in September (14.7%) and August (13.1%) and the smallest peaks were in March (3.2%) and January (3.8%). The dynamic population of DM in military areas almost followed the same pattern (Figure 1). Most DMs were found in the lieutenant dormitory (84.3%) and just 1.9% of DMs were found in the administrative place (Figure 2). Most of the mites were isolated from the beds (70.7%) and smaller numbers from the chairs (1.6). The results showed that the beds (1.82 mites per sample) and floors (0.75 mite per sample) were more infected place than the chairs (0.03 mite per sample) (Figure 2).

Seasonal abundance of DMs in three military areas in 2017 - 2018.
Seasonal abundance of DMs in three military areas in 2017 - 2018.
Figure 1.

Seasonal abundance of DMs in three military areas in 2017 - 2018.

The prevalence of DMs in different zone samples of the military area
The prevalence of DMs in different zone samples of the military area
Figure 2.

The prevalence of DMs in different zone samples of the military area

5. Discussion

In this study, Dermatophagoides farina was the most dominant species with a frequency of 82.8% of the pyroglyphid mites and 72.7% of the total mites’ population. D. pteronyssinus with 14.57% was the second most frequent species. Almost all studies on the HDMs in Iran have confirmed that the most dominant HDMs species in Iran is D. pteronyssinus such as a study that have been done in the homes of Sari city, Mazandaran province (the Guilan province neighbor) in Iran, that reported D. pteronyssinus (87.75%) as the main species and D. farina (1.15%) as the second most frequent species (19). Just a study on level of house dust mite allergens in Sari and Gorgan cities revealed the level of Der p1 was less than Der f1 and they mentioned that D. farina is more resistant than D. pteronyssinus to variable weather, causing more density of D. farinae (21). There are two probabilities for this inconsistency: (1) the special condition of military areas dormitory such as the strict rule for cleaning and used material for dormitory building, has been in favor of D. farinae and in the lack of D. pteronyssinus; (2) the latest morphological research on dust mites in the South shore of the Caspian Sea dates backs to 16 years ago (19), and changing in dust mites biodiversity over time have been reported by Halmai, and Petrova and Zheltikova (22, 23). According to the Fereidouni et al. and our finding, the second one is more probable (21).

The third most frequent species was B. tropicalis (7.02%). This species is a storage mite and the most common and abundant species in tropical and subtropical regions of the world (23, 24). The pyroglyphid mites and the storage mite such as B. tropicalis are the main sources of allergens in house dust (25). Also, studies on indoor allergen in Iran confirmed that those species are one of the main sources of indoor allergens in Iran (20, 21, 26-28). The presence of all of these species in military areas dormitory showed that doing preventive control was mandatory.

According to the results, most DMs were found in the lieutenant dormitory and 13.6% of DM were found in the soldier dormitory. The reasons for the higher prevalence of DMs in the lieutenant dormitory than the soldier dormitory can be as follows: (1) type of floor coverings (the lieutenant dormitory floor has been covered with carpet, but the soldier dormitory has been covered with natural stone tiles, except for the lieutenant dormitory of second area that a small part of floor has been covered with carpet); (2) cleaning regulations (there were stricter cleaning regulations in the soldier dormitory than the lieutenant dormitory); (3) the possibility to bring personal blankets and pillows from outside of military areas to the lieutenant dormitory (it is prohibited in the soldier dormitory except for the lieutenant dormitory of second area). Studies on the efficacy of the type of the floor on the density of house dust mites revealed the water resistant materials such as wooden and natural stone tile are the best for floor coverage in the place with a chance of mite outbreak because the water-resistant materials are easily washed and reduce the indoor humidity (5, 29).

The prevalence of DMs in the second military area was less than other naval bases, the main difference was related to the difference in the prevalence of DMs in the lieutenant dormitories. Regulations and rules of the lieutenant dormitory of the second area were as good as the soldier dormitory (but there were not in the other lieutenant dormitories). Also, a small part of the lieutenant dormitory of the second area was covered with a carpet that cleaned every day (but in the other lieutenant dormitories a carpet covered the whole of the floor).

5.1. Conclusions

In summary, the results of this study show that all military areas dormitories are contaminated with more than four allergen mite species. Three main allergenic dust mite species D. farinae, D. pteronyssinus, and Blomia tropicalis were the most prevalent species in the studied area. The prevalence of DMs was near the risky threshold in the first and third military areas from June to November. Regarding the high prevalence of DMs in those military areas, we suggest that the following preventive measures will be done in first and third military areas from June to November: weekly monitoring of allergen mite population, indoor temperature and humidity, weekly cleaning all surfaces by vacuum cleaner in full power (especially pillows, mattress, and carpets), implementation of strict cleaning regulation, and avoiding of leaving dirty clothes under beds.

Acknowledgements

References

  • 1.

    Platts-Mills TA, Chapman MD. Dust mites: Immunology, allergic disease, and environmental control. J Allergy Clin Immunol. 1987;80(6):755-75. [PubMed ID: 3320157].

  • 2.

    Fernandez-Caldas E, Puerta L, Caraballo L. Mites and allergy. History of allergy. Karger Publishers; 2014.

  • 3.

    Yong TS, Jeong KY. Household arthropod allergens in Korea. Korean J Parasitol. 2009;47 Suppl:S143-53. [PubMed ID: 19885330]. [PubMed Central ID: PMC2769222]. https://doi.org/10.3347/kjp.2009.47.S.S143.

  • 4.

    Milian E, Diaz AM. Allergy to house dust mites and asthma. P R Health Sci J. 2004;23(1):47-57. [PubMed ID: 15125219].

  • 5.

    Biddulph P. Colloff, M.J.: Dust Mites. Experimental and applied acarology. 2010;52(4):449-50. https://doi.org/10.1007/s10493-010-9407-3.

  • 6.

    Kawamoto S, Aki T, Yamashita M, Tategaki A, Fujimura T, Tsuboi S, et al. Toward elucidating the full spectrum of mite allergens--state of the art. J Biosci Bioeng. 2002;94(4):285-98. [PubMed ID: 16233305].

  • 7.

    Nuttall TJ, Hill PB, Bensignor E, Willemse T; Members of the International Task Force on Canine Atopic Dermatitis. House dust and forage mite allergens and their role in human and canine atopic dermatitis. Vet Dermatol. 2006;17(4):223-35. [PubMed ID: 16827665]. https://doi.org/10.1111/j.1365-3164.2006.00532.x.

  • 8.

    Arlian LG. Arthropod allergens and human health. Annu Rev Entomol. 2002;47:395-433. [PubMed ID: 11729080]. https://doi.org/10.1146/annurev.ento.47.091201.145224.

  • 9.

    Ferguson P, Broide DH. Environmental and bronchoalveolar lavage Dermatophagoides pteronyssinus antigen levels in atopic asthmatics. Am J Respir Crit Care Med. 1995;151(1):71-4. [PubMed ID: 7812575]. https://doi.org/10.1164/ajrccm.151.1.7812575.

  • 10.

    Arlian LG. House-dust-mite allergens: A review. Exp Appl Acarol. 1991;10(3-4):167-86. [PubMed ID: 2044430].

  • 11.

    Stewart GA, Robinson C. The immunobiology of allergenic peptidases. Clin Exp Allergy. 2003;33(1):3-6. https://doi.org/10.1046/j.1365-2222.2003.01601.x.

  • 12.

    Fereidouni M, Fereidouni F, Hadian M, Asghari Z, Zojaji SM. The Level of mite dermatophagoides' allergens (Der-p 1 and Der-f 1) in Birjand. Zahedan J Res Med Sci. 2014;16(4):38-40.

  • 13.

    Soleimani M, Rafinejad J. House dust mite contamination in hotels and inns in Bandar Abbas, South of Iran. J Environ Health Sci Eng. 2008;5(3):207-10.

  • 14.

    Soltani A, Azizi K, Saleh V, Dabaghmanesh T. The fauna and distribution of house dust mites in residential homes of Bandar Abbas district, Southern Iran. Exp Appl Acarol. 2011;54(3):269-76. [PubMed ID: 21380755]. https://doi.org/10.1007/s10493-011-9436-6.

  • 15.

    Soleimani-Ahmadi M, Zare M, Abtahi SM, Khazeni A. Species identification and prevalence of house dust mites as respiratory allergen in kindergartens of the Bandar Abbas city. Iran J Allergy Asthma Immunol. 2017;16(2):133-9.

  • 16.

    Ziyaei T, Berenji F, Jabbari-Azad F, Fata A, Jarahi L, Fereidouni M. House dust mite prevalence in the house of patients with atopic dermatitis in Mashhad, Iran. J Arthropod Borne Dis. 2017;11(2):309-14. [PubMed ID: 29062855]. [PubMed Central ID: PMC5641619].

  • 17.

    Amoli K, Cunnington AM. House dust mites in Iran. Clin Exp Allergy. 1977;7(1):93-101. https://doi.org/10.1111/j.1365-2222.1977.tb01429.x.

  • 18.

    Sepasgosarian H, Mumcuoglu Y. [Faunistic and ecological studies of house dust mites in Iran]. Int J Acarol. 1979;5(2):131-8. German. https://doi.org/10.1080/01647957908683136.

  • 19.

    Haghi F, Sharif M, Esmaeli R, Rafinejad J, Parsi B. [Identification of differant species of mites in dust, collected from residents of sari township in 1999-2000]. J Mazandaran Univ Med Sci. 2003;13(38):54-8. Persian.

  • 20.

    Assarehzadegan MA, Shakurnia A, Amini A. The most common aeroallergens in a tropical region in Southwestern Iran. World Allergy Organ J. 2013;6(1):7. [PubMed ID: 23663517]. [PubMed Central ID: PMC3651228]. https://doi.org/10.1186/1939-4551-6-7.

  • 21.

    Fereidouni M, Fereidouni F, Hadian M, Nourani Hasankiadeh S, Mazandarani M, Ziaee M. Evaluation of the level of house dust mite allergens, Der p 1 and Der f 1 in Iranian homes, a nationwide study. Allergol Immunopathol (Madr). 2013;41(6):381-6. [PubMed ID: 23485047]. https://doi.org/10.1016/j.aller.2012.10.013.

  • 22.

    Halmai ZS. Changes in the composition of house-dust mite fauna in Hungary. Parasit Hung. 1984;17:59-70.

  • 23.

    Petrova AD, Zheltikova TM. Long-term dynamics and structure of house dust mite community in Moscow (Acariformes, Astigmata). Zoologicheskii Zhurnal. 2000;79(12):1402-8.

  • 24.

    Fernandez-Caldas E, Lockey RF. Blomia tropicalis, a mite whose time has come. Allergy. 2004;59(11):1161-4. [PubMed ID: 15461595]. https://doi.org/10.1111/j.1398-9995.2004.00727.x.

  • 25.

    Arlian LG, Bernstein D, Bernstein IL, Friedman S, Grant A, Lieberman P, et al. Prevalence of dust mites in the homes of people with asthma living in eight different geographic areas of the United States. J Allergy Clin Immunol. 1992;90(3 Pt 1):292-300. [PubMed ID: 1527314].

  • 26.

    Farhoudi A, Razavi A, Chavoshzadeh Z, Heidarzadeh M, Bemanian MH, Nabavi M. Descriptive study of 226 patients with allergic rhinitis and asthma. Iran J Allergy Asthma Immunol. 2005:99-102.

  • 27.

    Fereidouni M, Hossini RF, Azad FJ, Assarehzadegan MA, Varasteh A. Skin prick test reactivity to common aeroallergens among allergic rhinitis patients in Iran. Allergol Immunopathol (Madr). 2009;37(2):73-9. [PubMed ID: 19445863].

  • 28.

    Goronfolah L. Aeroallergens, atopy and allergic rhinitis in the Middle East. Eur Ann Allergy Clin Immunol. 2016;48(1):5-21. [PubMed ID: 26808447].

  • 29.

    Wahongan GJ, Sembel DT, Tulung M, Baskoro T. Types and density of dust mites found among different habitats in houses in North Sulawesi province, Indonesia. J Entomol Zool Stud. 2017;5(2):681-5.