https://doi.org/10.5812/archneurosci.18214

Nerve Injury-Induced Plasticity in the Nociceptive Pathways

authors:

avatar Zahra Bahari 1 , avatar Seyed Shahabeddin Sadr 2 , * , avatar Gholam Hossein Meftahi 3 , avatar Maedeh Ghasemi 4 , avatar Homa Manaheji 1 , avatar Alireza Mohammadi 3 , avatar Nasrin Mehranfard 2

Department of Neurophysiology, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran
Electrophysiology Research Center, Neuroscience Institute, Tehran University of Medical Sciences, Tehran, IR Iran
Neuroscience Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran
Department of Physiology, Isfahan University of Medical Sciences, Isfahan, IR Iran
Archives of Neuroscience: Vol.2, issue 2; e18214
published online: June 30, 2014
article type: Review Article
received: February 23, 2014
revised: June 8, 2014

how to cite: Bahari Z, Sadr S S, Meftahi G H, Ghasemi M, Manaheji H, et al. Nerve Injury-Induced Plasticity in the Nociceptive Pathways. Arch Neurosci. 2015;2(2):e18214. https://doi.org/10.5812/archneurosci.18214.

Abstract

Context:

Neuropathic pain is a common and disabling complication. To develop a better treatment of the neuropathic pain, a comprehensive understanding is essential. In this paper, we review pathophysiological principles of neuropathic pain, focusing on synaptic plasticity and long-term potentiation (LTP) in the nociceptive circuits. Finally, the role of glial cells on the synaptic plasticity in neuropathic pain is discussed.

Evidence Acquisition:

We searched the Cochrane and PubMed databases using the following terms: neuropathic pain, dorsal horn, LTP, synaptic plasticity, nociceptive circuits, glial cells, nerve injury, allodynia, hyperalgesia, nociceptive neurons, and rat. All of searches were limited to the animal studies in English articles. Full-text copies were obtained when the studies had possible relevance.

Results:

Analysis of our research showed that nerve injury-induced LTP decreased pain threshold and increased pain hypersensitivity to sub-threshold stimuli. In addition, cross talk between dorsal horn neurons and glial cells are pivotal for the induction of spinal synaptic plasticity and LTP.

Conclusions:

It seems that LTP in the spinal nociceptive pathways constitutes cellular mechanisms that explains how acute pain may become chronic.

1. Context

Neuropathic pain is a common and severely disabling complication that impairs quality of life. The treatment of neuropathic pain is problematic because it is very complex, and the underlying mechanisms are not clearly understood (1). In recent years, attention was focused on synaptic plasticity in the dorsal horn as an important mechanism for neuropathic pain. One important type of the synaptic plasticity is LTP in the spinal neural system (2). Therefore, targeting maladaptive neuronal plasticity and LTP in the spinal pathways is an important way for the treatment of neuropathic pain. Moreover, recently it has been reported that glial cells are critical for the development and maintenance of synaptic plasticity (3). The aim of this review is to investigate recent progress in the neuropathic pain pathophysiology. We focus on the synaptic plasticity and LTP in nociceptive circuits. Finally, the cross talk between neuronal and glial cells in neuropathic pain is discussed.

2. Evidence Acquisition

We searched the Cochrane and PubMed database without date limitation, using the following terms: neuropathic pain, dorsal horn, LTP, synaptic plasticity, nociceptive circuits, glial cells, nerve injury, allodynia, hyperalgesia, nociceptive neurons, and rat. Following this search, we conducted a backward search by examining reference lists of all obtained articles. The searches were limited to the animals in English language. We checked all titles and abstracts. Full-text copies were obtained when the studies had possible relevance.

3. Results

3.1. Neuropathic Pain

Clinical pain can arise from damage to the nervous system (neuropathic pain), inflammation, or tissue injury (inflammatory pain). Etiology of neuropathic pain is different and includes nerve injury (e.g. amputation, spinal cord injury) or diseases that affect peripheral nerve function (e.g. metabolic disorders, viral infection and cancer). The most distinctive signs of neuropathic pain are hyperalgesia (an exaggerated response to painful stimuli), allodynia (the presence of pain in response to normally non-painful tactile stimuli) and spontaneous pain (stimulus independent pain) (4-6). The dorsal horn of the spinal cord is the first and major site for the integration and modulation of the pain signals. In neuropathic conditions, nerve injury induces ectopic firing of action potentials (ongoing activity) in the site of injury that develops to the dorsal horn, leading to synaptic plasticity. Ectopic action potentials also developed in uninjured fibers, and increased sensitivity to the pain (7-9). All of these states have important roles in the development and maintenance of neuropathic behaviors such as allodynia and hyperalgesia (10-14).

3.2. Short-Term and Long-Term Plasticity in Spinal Synapses

Recent studies have increasingly shown that synaptic strength is not fixing and vary in response to the changes of transmitter release from presynaptic terminals and the postsynaptic neuron activity. The variation of synaptic strength constitutes a mechanism known as synaptic plasticity (15, 16). Depending on the type of synapse and the intensity, frequency, and duration of the stimulus, both increase (potentiation or facilitation) and decrease (depression) in synaptic strength can be induced. In the animal model studies, nociceptive spinal connections exhibit short-term potentiation (STP or wind-up), long-term potentiation (LTP) and long-term depression (LTD) (2, 17). Wind-up is characterized by a progressive potentiation in the number of action potential from dorsal horn neurons during a train of repeated low frequency stimulation of C-fiber (16 stimuli, 5Hz). Second type of the synaptic plasticity is long-term potentiation (LTP). LTP is defined as a long-lasting responsiveness of dorsal horn neurons (18).

LTP was first detected at the hippocampus synapses as a model of learning and memory formation (19). Subsequent studies showed that LTP can also be induced in spinal pain pathways and may contribute to the neuropathic behaviors such as hyperalgesia and allodynia (20-22). LTP can induce in the superficial dorsal horn by field potentials recording (21) or patch clamp techniques (23), and even in the deep single WDR neurons by in vivo single-unit recording (24, 25). Ikeda et al. have shown that electrical high-frequency stimulation (HFS, 100Hz) of dorsal root induces LTP in the NK1-expressing neurons in laminae I (26). Moreover, low frequency stimulation (LFS, 2Hz) induces LTP in the laminae I neurons (27). In addition, LTP can be induced in the deep laminae of the spinal cord (e.g. in laminae IV-VI), where mainly WDR neurons are located (24, 25). The contribution of the WDR neurons in the nociceptive plasticity is less clear; however, many studies reported that these neurons have a pivotal role in the LTP.

For the first time, Svendsen et al. showed that electrical HFS of the sciatic nerve induces LTP in the evoked C-fiber responses from single WDR neurons in the deep spinal dorsal horn using in vivo extracellular single-unit recording (24, 25, 28). It seems that spinal LTP, injury-induced hyperalgesia and allodynia share common signaling pathways, time course and pharmacological profiles (21, 29). Taking together, LTP can be induced throughout the dorsal horn (superficial and deep lamina) and is an attractive model for injury-evoked painful behaviors such as allodynia and hyperalgesia. At least two different stages of LTP, depending upon its duration and signaling pathways, can be identified. Early phase LTP only required post translational modifications and lasted up to three hours. Late phase LTP required gene transcription and probably lasted up to the life span of an animal (30).

3.3. Cellular Mechanisms of the LTP in Spinal Cord

Some studies have shown that activation of the N-methyl-D-aspartate (NMDA) receptors is critical for the induction of LTP in the dorsal horn. Application of NMDA receptor antagonists like ketamine suppresses induction of spinal LTP (23, 31). NMDA receptors are activated by several mechanisms following nerve injury, including:

1) Nerve injury reduced the glutamate transporter expression in the spinal cord, leading to elevation of synaptic glutamate concentration (32).

2) Nerve injury reduced the inhibitory GABAergic and serotonergic input onto dorsal horn neurons, leading to facilitation of synaptic strength (33, 34).

In addition to NMDA receptor, induction of LTP in the spinal cord requires activation of NK1 receptors, opening of T-type voltage-gated Ca2+ channels, and activation of group I metabotropic glutamate receptors (26, 27, 35). Activation of NK1 receptors by substance P directly increases activation of NMDA receptor in the laminae I neurons and so increases postsynaptic intracellular Ca2+ level (36). Several signaling pathways modulate Ca2+ concentration in neuropathic pain such as activation of protein kinase A (PKA), protein kinase C (PKC), phospholipase C (PLC), inositol triphosphate-3 (IP3) receptors, calcium/calmodulin-dependent protein kinase II (CaMKII), nitric oxide synthase (NOS), mitogen-activated protein kinase (MAPK), and extracellular signal regulated kinase (ERK) (37-39). Studies using voltage sensitive dyes have shown that the potentiation of the electrical activity in the primary afferents after induction of LTP is partially sensitive to an mGluR group I antagonist (LY367385), iNOS inhibitor (AMT), an inhibitor of glial metabolisms (MFA) and protein synthesis inhibitors (cycloheximide or anisomycin) (40, 41). Interestingly, the same signal transduction pathways is required for the initiation and development of neuropathic signs like hyperalgesia in animal models of neuropathic pain (42).

3.4. Role of Glial Cells in the Neuropathic Pain

Glial cells are generally divided into three main categories: (1) oligodendrocytes, located in the white matter and constitute the myelin sheath around axons; (2) astrocytes, which have an important role in metabolism of the neurons; and (3) microglial cells, which do not have a neural origin and are referred to as the immune cells of the brain (43). The oligodendrocytes, astrocytes and microglia are being activated in neuropathic pain, leading to neuronal hypersensitivity. It is reported that knock out of glial fibrillary acidic protein (GFAP) gene in the astrocytes and Bergmann glial cells (a type of the astrocyte in the cerebellum) impairs eye blink conditioning and LTD in the cerebellum (44). However, in the hippocampus, the lack of GFAP in the astrocytes induces an increase of LTP (44, 45). Moreover, the lack of GFAP in GFAP-null mice decreases the number of glutamate transporters both in the neurons and astrocytes (46). Thus, signaling pathways of the glial cells can modulate the synaptic strength. Several studies have shown that overexpression of S100b (a type of astrocytic calcium-binding protein) impaired spatial exploration and therefore, synaptic plasticity (47-50). Additionally, application of the fluoroacetate (an inhibitor of the glial metabolism) suppresses induction of LTP in the spinal cord. However, the role of glial cells in the synaptic potentiation is still not well understood.

4. Discussion

Neuropathic pain is a highly complex disorder, which requires more studies. LTP is considered as a cellular mechanism for neuropathic pain. Pain memory in dorsal horn is a suitable explanation for maintenance of hyperalgesia and allodynia even after complete nerve healing. We conclude that LTP in the spinal nociceptive system constitutes cellular mechanisms to explain how acute pain may become chronic. It seems that the synaptic memory in the nociceptive system is a principle topic for pain researchers.

Acknowledgements

References

  • 1.

    von Hehn CA, Baron R, Woolf CJ. Deconstructing the neuropathic pain phenotype to reveal neural mechanisms. Neuron. 2012;73(4):638-52. [PubMed ID: 22365541]. https://doi.org/10.1016/j.neuron.2012.02.008.

  • 2.

    Ji RR, Kohno T, Moore KA, Woolf CJ. Central sensitization and LTP: do pain and memory share similar mechanisms? Trends Neurosci. 2003;26(12):696-705. [PubMed ID: 14624855]. https://doi.org/10.1016/j.tins.2003.09.017.

  • 3.

    Milligan ED, Watkins LR. Pathological and protective roles of glia in chronic pain. Nat Rev Neurosci. 2009;10(1):23-36. [PubMed ID: 19096368]. https://doi.org/10.1038/nrn2533.

  • 4.

    McCarberg BH, Billington R. Consequences of neuropathic pain: quality-of-life issues and associated costs. Am J Manag Care. 2006;12(9 Suppl):S263-8. [PubMed ID: 16774458].

  • 5.

    Hulsebosch CE, Hains BC, Crown ED, Carlton SM. Mechanisms of chronic central neuropathic pain after spinal cord injury. Brain Res Rev. 2009;60(1):202-13. [PubMed ID: 19154757]. https://doi.org/10.1016/j.brainresrev.2008.12.010.

  • 6.

    Campbell JN, Meyer RA. Mechanisms of neuropathic pain. Neuron. 2006;52(1):77-92. [PubMed ID: 17015228]. https://doi.org/10.1016/j.neuron.2006.09.021.

  • 7.

    Amir R, Kocsis JD, Devor M. Multiple interacting sites of ectopic spike electrogenesis in primary sensory neurons. J Neurosci. 2005;25(10):2576-85. [PubMed ID: 15758167]. https://doi.org/10.1523/JNEUROSCI.4118-04.2005.

  • 8.

    Boucher TJ, Okuse K, Bennett DL, Munson JB, Wood JN, McMahon SB. Potent analgesic effects of GDNF in neuropathic pain states. Science. 2000;290(5489):124-7. [PubMed ID: 11021795].

  • 9.

    D'Mello R, Dickenson AH. Spinal cord mechanisms of pain. Br J Anaesth. 2008;101(1):8-16. [PubMed ID: 18417503]. https://doi.org/10.1093/bja/aen088.

  • 10.

    Burgess SE, Gardell LR, Ossipov MH, Malan TP, Jr, Vanderah TW, Lai J, et al. Time-dependent descending facilitation from the rostral ventromedial medulla maintains, but does not initiate, neuropathic pain. J Neurosci. 2002;22(12):5129-36. [PubMed ID: 12077208].

  • 11.

    Kovelowski CJ, Ossipov MH, Sun H, Lai J, Malan TP, Porreca F. Supraspinal cholecystokinin may drive tonic descending facilitation mechanisms to maintain neuropathic pain in the rat. Pain. 2000;87(3):265-73. [PubMed ID: 10963906].

  • 12.

    Vera-Portocarrero LP, Zhang ET, Ossipov MH, Xie JY, King T, Lai J, et al. Descending facilitation from the rostral ventromedial medulla maintains nerve injury-induced central sensitization. Neuroscience. 2006;140(4):1311-20. [PubMed ID: 16650614]. https://doi.org/10.1016/j.neuroscience.2006.03.016.

  • 13.

    Nickel FT, Seifert F, Lanz S, Maihofner C. Mechanisms of neuropathic pain. Eur Neuropsychopharmacol. 2012;22(2):81-91. [PubMed ID: 21672666]. https://doi.org/10.1016/j.euroneuro.2011.05.005.

  • 14.

    Sandkuhler J. Learning and memory in pain pathways. Pain. 2000;88(2):113-8. [PubMed ID: 11050365].

  • 15.

    Woolf CJ. Evidence for a central component of post-injury pain hypersensitivity. Nature. 1983;306(5944):686-8. [PubMed ID: 6656869].

  • 16.

    Sandkuhler J. Understanding LTP in pain pathways. Mol Pain. 2007;3:9. [PubMed ID: 17407590]. https://doi.org/10.1186/1744-8069-3-9.

  • 17.

    Ikeda H, Kiritoshi T, Murase K. Synaptic plasticity in the spinal dorsal horn. Neurosci Res. 2009;64(2):133-6. [PubMed ID: 19428692]. https://doi.org/10.1016/j.neures.2009.03.004.

  • 18.

    Rygh LJ, Tjolsen A, Hole K, Svendsen F. Cellular memory in spinal nociceptive circuitry. Scand J Psychol. 2002;43(2):153-9. [PubMed ID: 12004953].

  • 19.

    Bliss TV, Lomo T. Long-lasting potentiation of synaptic transmission in the dentate area of the anaesthetized rabbit following stimulation of the perforant path. J Physiol. 1973;232(2):331-56. [PubMed ID: 4727084].

  • 20.

    Pockett S. Long-term potentiation and depression in the intermediate gray matter of rat spinal cord in vitro. Neuroscience. 1995;67(4):791-8. [PubMed ID: 7675205].

  • 21.

    Randic M, Jiang MC, Cerne R. Long-term potentiation and long-term depression of primary afferent neurotransmission in the rat spinal cord. J Neurosci. 1993;13(12):5228-41. [PubMed ID: 8254370].

  • 22.

    Sandkuhler J, Liu X. Induction of long-term potentiation at spinal synapses by noxious stimulation or nerve injury. Eur J Neurosci. 1998;10(7):2476-80. [PubMed ID: 9749775].

  • 23.

    Mao J. Translational pain research: bridging the gap between basic and clinical research. Pain. 2002;97(3):183-7. [PubMed ID: 12044614].

  • 24.

    Svendsen F, Rygh LJ, Gjerstad J, Fiska A, Hole K, Tjolsen A. Recording of long-term potentiation in single dorsal horn neurons in vivo in the rat. Brain Res Brain Res Protoc. 1999;4(2):165-72. [PubMed ID: 10446411].

  • 25.

    Svendsen F, Hole K, Tjolsen A. Long-term potentiation in single wide dynamic range neurons induced by noxious stimulation in intact and spinalized rats. Prog Brain Res. 2000;129:153-61. [PubMed ID: 11098687]. https://doi.org/10.1016/S0079-6123(00)29011-0.

  • 26.

    Ikeda H, Heinke B, Ruscheweyh R, Sandkuhler J. Synaptic plasticity in spinal lamina I projection neurons that mediate hyperalgesia. Science. 2003;299(5610):1237-40. [PubMed ID: 12595694]. https://doi.org/10.1126/science.1080659.

  • 27.

    Ikeda H, Stark J, Fischer H, Wagner M, Drdla R, Jager T, et al. Synaptic amplifier of inflammatory pain in the spinal dorsal horn. Science. 2006;312(5780):1659-62. [PubMed ID: 16778058]. https://doi.org/10.1126/science.1127233.

  • 28.

    Wallin J, Fiska A, Tjolsen A, Linderoth B, Hole K. Spinal cord stimulation inhibits long-term potentiation of spinal wide dynamic range neurons. Brain Res. 2003;973(1):39-43. [PubMed ID: 12729951].

  • 29.

    Liu XG, Sandkuhler J. Long-term potentiation of C-fiber-evoked potentials in the rat spinal dorsal horn is prevented by spinal N-methyl-D-aspartic acid receptor blockage. Neurosci Lett. 1995;191(1-2):43-6. [PubMed ID: 7659287].

  • 30.

    Bailey CH, Kandel ER, Si K. The persistence of long-term memory: a molecular approach to self-sustaining changes in learning-induced synaptic growth. Neuron. 2004;44(1):49-57. [PubMed ID: 15450159]. https://doi.org/10.1016/j.neuron.2004.09.017.

  • 31.

    Pud D, Eisenberg E, Spitzer A, Adler R, Fried G, Yarnitsky D. The NMDA receptor antagonist amantadine reduces surgical neuropathic pain in cancer patients: a double blind, randomized, placebo controlled trial. Pain. 1998;75(2-3):349-54. [PubMed ID: 9583771].

  • 32.

    Sung B, Lim G, Mao J. Altered expression and uptake activity of spinal glutamate transporters after nerve injury contribute to the pathogenesis of neuropathic pain in rats. J Neurosci. 2003;23(7):2899-910. [PubMed ID: 12684477].

  • 33.

    Moore KA, Kohno T, Karchewski LA, Scholz J, Baba H, Woolf CJ. Partial peripheral nerve injury promotes a selective loss of GABAergic inhibition in the superficial dorsal horn of the spinal cord. J Neurosci. 2002;22(15):6724-31. [PubMed ID: 12151551].

  • 34.

    Coull JA, Boudreau D, Bachand K, Prescott SA, Nault F, Sik A, et al. Trans-synaptic shift in anion gradient in spinal lamina I neurons as a mechanism of neuropathic pain. Nature. 2003;424(6951):938-42. [PubMed ID: 12931188]. https://doi.org/10.1038/nature01868.

  • 35.

    Azkue JJ, Liu XG, Zimmermann M, Sandkuhler J. Induction of long-term potentiation of C fibre-evoked spinal field potentials requires recruitment of group I, but not group II/III metabotropic glutamate receptors. Pain. 2003;106(3):373-9. [PubMed ID: 14659520].

  • 36.

    Lieberman DN, Mody I. Substance P enhances NMDA channel function in hippocampal dentate gyrus granule cells. J Neurophysiol. 1998;80(1):113-9. [PubMed ID: 9658033].

  • 37.

    Zhang XC, Zhang YQ, Zhao ZQ. Involvement of nitric oxide in long-term potentiation of spinal nociceptive responses in rats. Neuroreport. 2005;16(11):1197-201. [PubMed ID: 16012348].

  • 38.

    Xin WJ, Gong QJ, Xu JT, Yang HW, Zang Y, Zhang T, et al. Role of phosphorylation of ERK in induction and maintenance of LTP of the C-fiber evoked field potentials in spinal dorsal horn. J Neurosci Res. 2006;84(5):934-43. [PubMed ID: 16902997]. https://doi.org/10.1002/jnr.21013.

  • 39.

    Yang HW, Hu XD, Zhang HM, Xin WJ, Li MT, Zhang T, et al. Roles of CaMKII, PKA, and PKC in the induction and maintenance of LTP of C-fiber-evoked field potentials in rat spinal dorsal horn. J Neurophysiol. 2004;91(3):1122-33. [PubMed ID: 14586032]. https://doi.org/10.1152/jn.00735.2003.

  • 40.

    Ikeda H, Murase K. Glial nitric oxide-mediated long-term presynaptic facilitation revealed by optical imaging in rat spinal dorsal horn. J Neurosci. 2004;24(44):9888-96. [PubMed ID: 15525773]. https://doi.org/10.1523/JNEUROSCI.2608-04.2004.

  • 41.

    Hu NW, Zhang HM, Hu XD, Li MT, Zhang T, Zhou LJ, et al. Protein synthesis inhibition blocks the late-phase LTP of C-fiber evoked field potentials in rat spinal dorsal horn. J Neurophysiol. 2003;89(5):2354-9. [PubMed ID: 12740398]. https://doi.org/10.1152/jn.01027.2002.

  • 42.

    Willis WD. Role of neurotransmitters in sensitization of pain responses. Ann N Y Acad Sci. 2001;933:142-56. [PubMed ID: 12000017].

  • 43.

    Fields RD. White matter in learning, cognition and psychiatric disorders. Trends Neurosci. 2008;31(7):361-70. [PubMed ID: 18538868]. https://doi.org/10.1016/j.tins.2008.04.001.

  • 44.

    Shibuki K, Gomi H, Chen L, Bao S, Kim JJ, Wakatsuki H, et al. Deficient cerebellar long-term depression, impaired eyeblink conditioning, and normal motor coordination in GFAP mutant mice. Neuron. 1996;16(3):587-99. [PubMed ID: 8785056].

  • 45.

    McCall MA, Gregg RG, Behringer RR, Brenner M, Delaney CL, Galbreath EJ, et al. Targeted deletion in astrocyte intermediate filament (Gfap) alters neuronal physiology. Proc Natl Acad Sci U S A. 1996;93(13):6361-6. [PubMed ID: 8692820].

  • 46.

    Hughes EG, Maguire JL, McMinn MT, Scholz RE, Sutherland ML. Loss of glial fibrillary acidic protein results in decreased glutamate transport and inhibition of PKA-induced EAAT2 cell surface trafficking. Brain Res Mol Brain Res. 2004;124(2):114-23. [PubMed ID: 15135219]. https://doi.org/10.1016/j.molbrainres.2004.02.021.

  • 47.

    Janus C, Janus M, Roder J. Spatial exploration in transgenic mice expressing human beta-S100. Neurobiol Learn Mem. 1995;64(1):58-67. [PubMed ID: 7582813]. https://doi.org/10.1006/nlme.1995.1044.

  • 48.

    Roder JK, Roder JC, Gerlai R. Conspecific exploration in the T-maze: abnormalities in S100 beta transgenic mice. Physiol Behav. 1996;60(1):31-6. [PubMed ID: 8804639].

  • 49.

    Barger SW, Van Eldik LJ. S100 beta stimulates calcium fluxes in glial and neuronal cells. J Biol Chem. 1992;267(14):9689-94. [PubMed ID: 1315768].

  • 50.

    Roumier A, Pascual O, Bechade C, Wakselman S, Poncer JC, Real E, et al. Prenatal activation of microglia induces delayed impairment of glutamatergic synaptic function. PLoS One. 2008;3(7). ee2595. [PubMed ID: 18612411]. https://doi.org/10.1371/journal.pone.0002595.