article information

Archives of Pediatric Infectious Diseases: Vol.4, issue 1; e26639
published online: January 16, 2016
article type: Research Article
received: January 3, 2015
revised: November 2, 2015
accepted: November 14, 2015
DOI: https://doi.org/10.5812/pedinfect.26639
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Helicobacter pylori Seropositivity in Children With Asthma

authors:

avatar Parsa Yousefichaijan 1 , avatar Ghasem Mosayebi 2 , avatar Mojtaba Sharafkhah 3 , * , avatar Manijeh Kahbazi 4 , avatar Phaezeh Heydarbagi 3 , avatar Mohammad Rafiei 5

Department of Pediatric Nephrology, Arak University of Medical Sciences, Arak, IR Iran
Department of Immunology, Arak University of Medical Sciences, Arak, IR Iran
Students Research Committee, School of Medicine, Arak University of Medical Sciences, Arak, IR Iran
Department of Pediatric, Arak University of Medical Sciences, Arak, IR Iran
Department of Biostatistics and Epidemiology, Arak University of Medical Sciences, Arak, IR Iran

how to cite: Yousefichaijan P, Mosayebi G, Sharafkhah M, Kahbazi M, Heydarbagi P, et al. Helicobacter pylori Seropositivity in Children With Asthma. Arch Pediatr Infect Dis. 2016;4(1):e26639. https://doi.org/10.5812/pedinfect.26639.

Abstract

Background:

Some studies have reported an association between Helicobacter pylori (H. pylori) colonization and the occurrence of asthma or other allergies. However, data are inconsistent, and few studies have been performed in children.

Objectives:

The current study aimed to investigate H. pylori seropositivity in children with and without asthma.

Patients and Methods:

This cross-sectional study was performed with 208 children aged 5- to 12-years-old (104 asthmatic subjects in the case group and 104 healthy individuals in the control group) who had been referred to the pediatric clinic of Amir Kabir hospital in Arak, Iran. H. pylori seropositivity was compared between the two groups according to the serum level of immunoglobulin G (IgG) antibody against the H. pylori cytotoxin-associated gene A (CagA) protein, which was measured using the enzyme-linked immunosorbent assay (ELISA).

Results:

The H. pylori serology test was positive in 13 (12.5%) asthmatic subjects and 18 (17.3%) healthy subjects. This difference was not significant (P = 0.54). Duration of asthma in the serology-positive children (3.61 ± 1.5 years) was significantly higher than that of the serology-negative children (2.16 ± 1.33 years) (P = 0.0001). No significant correlations were found between H. pylori seropositivity and age (P = 0.95), gender (P = 0.7), severity of asthma (P = 0.54), control of the asthma (P = 0.23), or pulmonary function test (PFT) results (P = 0.23).

Conclusions:

The results showed no association between childhood asthma and H. pylori seropositivity. However, due to the lack of studies, especially in children, and controversial results of the clinical studies, further studies are recommended.

1. Background

Asthma is a common chronic childhood disease, and it is one of the most common causes of hospitalization among children (1). Asthma is associated with genetic risk factors and predisposing environmental factors (1), which often begin during the early years of life (1).

Several factors, such as air pollution, exposure to cigarette smoke, external infections, microbial substances in the environment, ownership of furry pets, and obesity, play a role in the development of asthma (2). According to the hygiene hypothesis, people’s reduced risk of and exposure to bacterial infections during childhood can cause atopic disorders, including asthma (3). Evidence suggests that early exposure to orofecal microbes, such as hepatitis A, can be a protective factor against allergic reactions (4). Moreover, according to this hypothesis, there is an inverse relationship of toxoplasma and Helicobacter pylori (H. pylori) with atopy (5).

H. pylori is a gram-negative bacterium that colonizes the stomach in more than half of the general population (6). It causes a variety of diseases, such as gastritis, peptic ulcer disease, gastric cancer, and lymphoid tissue lymphomas (7). It has recently been found that there is an inverse relationship between H. pylori and risk of gastroesophageal reflux disease (GERD) (8, 9). Given that there is a relationship between asthma and risk of GERD in some patients (10), this has led some authors to suggest that H. pylori might act as a protective factor against GERD-related asthma. In agreement with the hygiene hypothesis (3) and the notion of GERD-related asthma (10), an inverse association has been observed in some studies (10, 11) between H. pylori colonization and the occurrence of asthma or allergy.

2. Objectives

Since existing data are inconsistent and few studies have been performed in children (12), the aim of this study was to investigate H. pylori seropositivity in children with and without asthma.

3. Patients and Methods

During this cross-sectional study, which took place from November 2013 to May 2014, 104 five- to twelve-year-old children with documented asthma (for at least one year) were enrolled as a case group, and 104 age-matched healthy children were enrolled as a control group. All the subjects had been referred to our pediatric clinic of asthma and allergy and the general pediatric clinic of Amir Kabir hospital in Arak, Iran. Subjects who met the inclusion and exclusion criteria were selected based on simple probability.

Asthma was defined based on the 2008 global initiative for asthma (GINA) definition, as follows: asthma is a heterogeneous disease, usually characterized by chronic airway inflammation. It is defined by a history of respiratory symptoms, such as wheeze, shortness of breath, chest tightness, and cough, that vary over time and in intensity, together with variable expiratory airflow limitation (13).

Asthma is difficult to diagnose in infants and young children, for whom testing of reversible airway obstruction is technically difficult. Therefore, wheezing is often used as a proxy for asthma in children in epidemiological studies (6). In this study, for better evaluation of the asthma diagnosis, wheezing and current physician-diagnosed asthma are used as a proxy for asthma in children (6). Diagnosis of asthma was confirmed by a physician by asking the children’s parents the following questions: 1) Has your child made a whistling noise while breathing in the last 12 months?; 2) Has your child ever been diagnosed with asthma by a physician?; 3) Has your child had asthma in the last 12 months?

Our exclusion criteria were: 1) an exacerbation of asthma in the preceding month for the case group; 2) history of peptic ulcer, cerebrovascular disease, ischemic heart disease, or respiratory diseases (except asthma in the case group); 3) documented H. pylori infection; 4) prior H. pylori eradication therapy; 5) consumption of acid-suppressive drugs (proton pump inhibitors (PPIs), H2 receptor antagonists, non-steroidal anti-inflammatory drugs (NSAIDs), and antibiotics (such as amoxicillin, tetracycline, metronidazole, and clarithromycin; for at least 1 - 2 weeks)) in the preceding six months for gastric or other problems; 6) history of congenital gastrointestinal (GI) anomalies, gastric surgery, gastric malignancies, or any significant GI problem; 7) history of chronic diseases, especially autoimmune disorders; and 8) recent exposure to any infectious disease.

Following primary evaluation regarding the exclusion/inclusion criteria and receipt of informed consent from children’s parents for participating in the study, the following information was recorded: baseline characteristics (age, gender, residence); clinical information of the asthmatic group (duration of asthma (years), severity and control status of the asthma, and pulmonary functional test (PFT) results). Severity of the asthma was classified as mild/intermittent or more severe. The PFT results were classified as normal or as indicative of obstructive disease. H. pylori seropositivity was defined as a positive result of the serum level of IgG antibody against the H. pylori surface cytotoxin-associated gene A (CagA) protein (14-16).

Peripheral venous blood was drawn from the children. The samples were centrifuged, and the serum isolated from them was placed at −20ºC prior to the serological test. In order to check for the presence of IgG antibody against the H. pylori surface CagA protein, all sera were analyzed using the enzyme-linked immunosorbent assay (ELISA) (Binding Site, UK), and IgG was measured according to the manufacturer’s protocol. When the concentration of IgG antibodies against H. pylori was greater than 20 U/mL or less than 12.5 U/mL, the result was considered positive or negative, respectively. The results of the antibody test were analyzed by a person unaware of the children’s asthma status.

The collected data were analyzed using SPSS software (statistical package for the social sciences, version 18.0, SPSS Inc., Chicago, IL, USA) and descriptive statistics methods for frequency determination. Numerical data are expressed as the mean ± standard deviation (SD) and were compared using the t-test. Categorical data are expressed as numbers (percentages) and were compared with the chi-square test. P values less than .05 were considered significant. This study was approved by The ethics committee of Arak University of Medical Sciences. In all stages of this study we were loyal to the Helsinki declaration principles, and we obtained written consent from all the participants, who were free to exit the study at any time.

4. Results

The baseline characteristics of the 208 children selected for the study are shown in Table 1. The distributions of age (P = 0.32), gender (P = 0.37), and residence (P = 0.08) of the children were not significantly different between the two groups. The mean serum level of IgG against the CagA protein was 8.46 ± 2.47 U/mL and 12.74 ± 9.41 U/mL in the asthmatic and control groups, respectively. This difference was not significant (P = 0.23). IgG against the CagA protein was present in 31 (14.9%) of the 208 subjects in the two groups, which shows that there was no significant difference between the children with 13 (12.5%) and without 18 (17.3%) asthma (P = 0.54) (Table 2).

Table 1.

Baseline Characteristics of the Study Groups

CharacteristicChildren With Asthma aHealthy Children aP Value b
Age, y c7.23 ± 3.36.5 ± 3.840.32
Gender d0.37
Male34 (32.6)40 (38.4)
Female70 (67.3)64 (61.5)
Residence d0.08
Urban74 (71.1 )69 (66.3)
Rural30 (28.8)35 (33.6)

aNumber of subjects = 104.

bP values less than 0.05 were considered significant.

cValues are presented as mean ± SD.

dValues are presented as No. (%).

Table 2.

Results of Serum Testing for IgG Antibody Against the H. pylori Surface CagA Protein in the Study Groupsa

IgG Serology Test ResultAsthmatic Group bHealthy Group bP Value
Positive c13 (12.5)18 (17.3)0.54
Negative d91 (87.5)86 (82.6)0.54

aValues are presented as No. (%).

bNumber of subjects = 104.

cIgG concentrations higher than 20 U/L.

dIgG concentrations less than 12.5 U/mL.

In the asthmatic group, the results of the H. pylori serology test were analyzed according to age, gender, severity and control status of the asthma, duration of the disease, and PFT results (Table 3). The duration of asthma in the serology-positive children (3.61 ± 1.5 years) was significantly higher than that of the serology-negative children (2.16 ± 1.33 years) (P = 0.0001). No significant correlations were found between H. pylori seropositivity and age (P = 0.95), gender (P = 0.7), severity of asthma (P = 0.54), controlled asthma (P = 0.23), or PFT results (P = 0.23).

Table 3.

H. pylori Serology Test Results Among Asthmatic Patientsa,b

CharacteristicSerology-Positive (n = 13)Serology-Negative (n = 91)P Value
Age7.38 ±1.757.35 ± 2.140.95
Gender0.7
Male7 (53.8)54 (59.3)
Female6 (46.1)37 (40.6)
Duration of disease, y3.61 ± 1.52.16 ± 1.330.0001
Control status of disease0.23
Controlled12 (92.3)71 (78)
Uncontrolled1 (7.6)20 (21.9)
Severity of disease0.54
Persistently mild9 (69.2)55 (60.4)
Moderate and severe4 (30.7)36 (39.5)
PFT0.23
Normal result12 (92.3)71 (78)
Obstructive disease1 (7.6)20 (21.9)

aAbbreviations: PFT, pulmonary function test.

bValues are presented as No. (%) or mean ± SD.

5. Discussion

This study shows that there is no significant difference in H. pylori seropositivity between children with and without asthma. However, there was an association between prolonged duration of disease in asthmatic children and H. pylori seropositivity. In contrast to our findings, various cross-sectional and case-control studies have shown an inverse relationship of asthma and allergic rhinitis with H. pylori colonization, especially for CagA+ strains (10, 11, 17). Based on the third national health and nutrition examination survey (NHANES III) (18) database, Chen et al. (10) showed that there is an inverse relationship between seropositivity for CagA+ strains of H. pylori and risk of asthma, especially in young people and those who suffered from asthma during childhood. Likewise, in a case-control study Reibman et al. (11) reported seropositivity for CagA+ strains of H. pylori in 47.1% of all study samples and showed that there is an inverse relationship between the risk of asthma and CagA seropositivity. Furthermore, Shiotani et al. (17) studied the prevalence of serum H. pylori IgG among 369 university students with a history of allergic diseases (allergic rhinoconjunctivitis, atopic dermatitis, urticaria, bronchial asthma, and mixed diseases) and 408 control subjects and found that the prevalence of H. pylori seropositivity in the allergic group was significantly lower than that of the control group.

However, similar to our study, a few studies have reported no association (19) or a weak inverse association (20) of H. pylori colonization with asthma and allergy. In a study by Jarvis et al. (19) in 2004, previous infection with hepatitis A or H. pylori was assessed in a community-based sample of young British adults, and associations of serum-specific IgE with environmental allergens, asthma-like symptoms, and hay fever were investigated. They found no evidence of a relationship between hepatitis A or H. pylori infection with lower levels of IgE sensitization, asthma, or hay fever. Furthermore, Tsang et al. (20) did not find any significant difference between people with and without asthma in terms of the presence of H. pylori-specific IgG. They also did not report any relationship between serum levels of IgG and forced expiratory volume in 1 second (FEV1 % predicted), forced vital capacity (FVC % predicted), or duration of asthma.

In a cross-sectional study by Karimi et al. (21) in Iran, H. pylori infection was compared between 98 asthmatic children and 98 healthy individuals. Unlike many studies, H. pylori infection was diagnosed using the urea breath test (UBT); nonetheless, UBT positivity was not significantly different between the asthmatic 18 (18.3%) and healthy 23 (23.3%) children. Furthermore, analysis of the asthmatic group revealed an association of H. pylori infection with age (P < 0.001) and duration of asthma (P = 0.010).

The hygiene hypothesis and the potential role of H. pylori in protecting against GERD-related asthma are among the hypotheses supporting an inverse relationship between asthma and H. pylori (3, 10). According to the hygiene hypothesis, microbial infections in early childhood might act as a protective factor against allergy and asthma (22). Although the mechanism of this hypothesis is not entirely clear, early stimulation of gut-associated lymphoid tissue by infectious microbes, may play a role, the gut being the initial location for mucosal immune maturation (23). The role of H. pylori in protecting against GERD-related asthma was proposed based on the following evidence: (1) Asthma can be a risk factor for GERD (24, 25), (2) GERD can play a role in stimulating asthma (24, 25), and (3) There might be an inverse relationship between H. pylori and GERD (8, 9). According to previous evidence, this hypothesis is important because GERD is often not examined due to being asymptomatic, especially in children (26, 27).

In contrast to the above hypotheses, some authors believe that H. pylori colonization of the gastric mucosa may stimulate the secretion of various proinflammatory substances, such as cytokines and acute phase proteins (28). Accordingly, a direct relationship was proposed between H. pylori and diseases characterized by inflammation, such as asthma. A study has reported have reported a higher prevalence of H. pylori compared to healthy individuals in chronic inflammatory diseases, such as rosacea, urticarial, and Henoch-Schonlein purpura (29). Tsang et al. (30) showed a higher prevalence of H. pylori seropositivity in patients with bronchiectasis (76%) when compared with healthy individuals. Similarly, we found an association between prolonged duration of disease in asthmatic children with a higher incidence of H. pylori seropositivity; however, we found no general association between childhood asthma and the H. pylori seropositivity.

One of the limitations of this study is that endoscopic biopsy was not used to diagnose H. pylori. According to some observations (15, 16), serology tests have variable sensitivity and specificity in diagnosing H. pylori in children. In some cases, the results of the serology test can be influenced by the duration of infection and the ability of the host to mount an immune response. Therefore, it was suggested that endoscopic biopsy be conducted for a more definite diagnosis of H. pylori infection. However, the serology test was used in the current study for the following reasons: (1) endoscopy is an invasive procedure, and there is no indication for this diagnostic method in the evaluation of children who do not have gastrointestinal symptoms; and (2) parents may have been unwilling for their child to undergo the procedure. Nevertheless, it is recommended that future studies be conducted using endoscopy to diagnose H. pylori in children with gastrointestinal symptoms so that more accurate results can be obtained.

Although many studies have been carried out on the relationship between asthma and H. pylori seropositivity and/or infection, further clinical and laboratory studies are recommended to be conducted in future due to (1) contradictory hypotheses on the relationship between H. pylori and asthma, (2) controversial results of the clinical studies, (3) lack of studies in children, and (4) lack of evidence in regard to the association between the clinical features of asthma and H. pylori.

Acknowledgements

References

  • 1.

    Weiland SK, Husing A, Strachan DP, Rzehak P, Pearce N. Climate and the prevalence of symptoms of asthma, allergic rhinitis, and atopic eczema in children. Occup Environ Med. 2004;61(7):609-15. [PubMed ID: 15208377].

  • 2.

    Blaser MJ, Chen Y, Reibman J. Does Helicobacter pylori protect against asthma and allergy? Gut. 2008;57(5):561-7. [PubMed ID: 18194986]. https://doi.org/10.1136/gut.2007.133462.

  • 3.

    Strachan DP. Family size, infection and atopy: the first decade of the "hygiene hypothesis". Thorax. 2000;55 Suppl 1:S2-10. [PubMed ID: 10943631].

  • 4.

    Bjorksten B. Effects of intestinal microflora and the environment on the development of asthma and allergy. Springer Semin Immunopathol. 2004;25(3-4):257-70. [PubMed ID: 15007630]. https://doi.org/10.1007/s00281-003-0142-2.

  • 5.

    Matricardi PM, Rosmini F, Riondino S, Fortini M, Ferrigno L, Rapicetta M, et al. Exposure to foodborne and orofecal microbes versus airborne viruses in relation to atopy and allergic asthma: epidemiological study. Bmj. 2000;320(7232):412-7. [PubMed ID: 10669445].

  • 6.

    Holster IL, Vila AM, Caudri D, den Hoed CM, Perez-Perez GI, Blaser MJ, et al. The impact of Helicobacter pylori on atopic disorders in childhood. Helicobacter. 2012;17(3):232-7. [PubMed ID: 22515362]. https://doi.org/10.1111/j.1523-5378.2012.00934.x.

  • 7.

    Cave DR. Chronic gastritis and Helicobacter pylori. Semin Gastrointest Dis. 2001;12(3):196-202. [PubMed ID: 11478752].

  • 8.

    Vaezi MF, Falk GW, Peek RM, Vicari JJ, Goldblum JR, Perez-Perez GI, et al. CagA-positive strains of Helicobacter pylori may protect against Barrett's esophagus. Am J Gastroenterol. 2000;95(9):2206-11. [PubMed ID: 11007219]. https://doi.org/10.1111/j.1572-0241.2000.02305.x.

  • 9.

    Vicari JJ, Peek RM, Falk GW, Goldblum JR, Easley KA, Schnell J, et al. The seroprevalence of cagA-positive Helicobacter pylori strains in the spectrum of gastroesophageal reflux disease. Gastroenterology. 1998;115(1):50-7. [PubMed ID: 9649458].

  • 10.

    Chen Y, Blaser MJ. Inverse associations of Helicobacter pylori with asthma and allergy. Arch Intern Med. 2007;167(8):821-7. [PubMed ID: 17452546]. https://doi.org/10.1001/archinte.167.8.821.

  • 11.

    Reibman J, Marmor M, Filner J, Fernandez-Beros ME, Rogers L, Perez-Perez GI, et al. Asthma is inversely associated with Helicobacter pylori status in an urban population. PLoS One. 2008;3(12). eee4060. [PubMed ID: 19112508]. https://doi.org/10.1371/journal.pone.0004060.

  • 12.

    Chen Y, Blaser MJ. Helicobacter pylori colonization is inversely associated with childhood asthma. J Infect Dis. 2008;198(4):553-60. [PubMed ID: 18598192]. https://doi.org/10.1086/590158.

  • 13.

    Bateman ED, Hurd SS, Barnes PJ, Bousquet J, Drazen JM, FitzGerald M, et al. Global strategy for asthma management and prevention: GINA executive summary. Eur Respir J. 2008;31(1):143-78. [PubMed ID: 18166595]. https://doi.org/10.1183/09031936.00138707.

  • 14.

    Crabtree JE, Mahony MJ, Taylor JD, Heatley RV, Littlewood JM, Tompkins DS. Immune responses to Helicobacter pylori in children with recurrent abdominal pain. J Clin Pathol. 1991;44(9):768-71. [PubMed ID: 1918408].

  • 15.

    Talley NJ. American Gastroenterological Association medical position statement: evaluation of dyspepsia. Gastroenterology. 2005;129(5):1753-5. [PubMed ID: 16285970]. https://doi.org/10.1053/j.gastro.2005.09.019.

  • 16.

    Jun ZJ, Lei Y, Shimizu Y, Dobashi K, Mori M. Helicobacter pylori seroprevalence in patients with mild asthma. Tohoku J Exp Med. 2005;207(4):287-91. [PubMed ID: 16272799].

  • 17.

    Shiotani A, Miyanishi T, Kamada T, Haruma K. Helicobacter pylori infection and allergic diseases: epidemiological study in Japanese university students. J Gastroenterol Hepatol. 2008;23(7 Pt 2):e29-33. [PubMed ID: 17725593]. https://doi.org/10.1111/j.1440-1746.2007.05107.x.

  • 18.

    Plan and operation of the Third National Health and Nutrition Examination Survey, 1988-94. Series 1: programs and collection procedures. Vital Health Stat 1. 1994;(32):1-407. [PubMed ID: 7975354].

  • 19.

    Jarvis D, Luczynska C, Chinn S, Burney P. The association of hepatitis A and Helicobacter pylori with sensitization to common allergens, asthma and hay fever in a population of young British adults. Allergy. 2004;59(10):1063-7. [PubMed ID: 15355464]. https://doi.org/10.1111/j.1398-9995.2004.00539.x.

  • 20.

    Tsang KW, Lam WK, Chan KN, Hu W, Wu A, Kwok E, et al. Helicobacter pylori sero-prevalence in asthma. Respir Med. 2000;94(8):756-9. [PubMed ID: 10955750]. https://doi.org/10.1053/rmed.2000.0817.

  • 21.

    Karimi A, Fakhimi-Derakhshan K, Imanzadeh F, Rezaei M, Cavoshzadeh Z, Maham S. Helicobacter pylori infection and pediatric asthma. Iran J Microbiol. 2013;5(2):132-5. [PubMed ID: 23825730].

  • 22.

    Crane J, Wickens K. Antibiotics and asthma: a tricky tributary of the hygiene hypothesis. Lancet Respir Med. 2014;2(8):595-7. [PubMed ID: 24835832]. https://doi.org/10.1016/s2213-2600(14)70109-2.

  • 23.

    Kelly D, Conway S, Aminov R. Commensal gut bacteria: mechanisms of immune modulation. Trends Immunol. 2005;26(6):326-33. [PubMed ID: 15922949]. https://doi.org/10.1016/j.it.2005.04.008.

  • 24.

    Kiljander TO, Laitinen JO. The prevalence of gastroesophageal reflux disease in adult asthmatics. Chest. 2004;126(5):1490-4. [PubMed ID: 15539717]. https://doi.org/10.1378/chest.126.5.1490.

  • 25.

    Harding SM. Gastroesophageal reflux: a potential asthma trigger. Immunol Allergy Clin North Am. 2005;25(1):131-48. [PubMed ID: 15579368]. https://doi.org/10.1016/j.iac.2004.09.006.

  • 26.

    el-Serag HB, Sonnenberg A. Opposing time trends of peptic ulcer and reflux disease. Gut. 1998;43(3):327-33. [PubMed ID: 9863476].

  • 27.

    Ronkainen J, Aro P, Storskrubb T, Johansson SE, Lind T, Bolling-Sternevald E, et al. High prevalence of gastroesophageal reflux symptoms and esophagitis with or without symptoms in the general adult Swedish population: a Kalixanda study report. Scand J Gastroenterol. 2005;40(3):275-85. [PubMed ID: 15932168].

  • 28.

    Negrini R, Savio A, Poiesi C, Appelmelk BJ, Buffoli F, Paterlini A, et al. Antigenic mimicry between Helicobacter pylori and gastric mucosa in the pathogenesis of body atrophic gastritis. Gastroenterology. 1996;111(3):655-65. [PubMed ID: 8780570].

  • 29.

    Kuipers EJ, Perez-Perez GI, Meuwissen SG, Blaser MJ. Helicobacter pylori and atrophic gastritis: importance of the cagA status. J Natl Cancer Inst. 1995;87(23):1777-80. [PubMed ID: 7473834].

  • 30.

    Tsang KW, Lam SK, Lam WK, Karlberg J, Wong BC, Hu WH. High seroprevalence of Helicobacter pylori in active bronchiectasis. Am J Respir Crit Care Med. 1998;158(4):1047-51.