Investigating the Epidemiologic, Laboratory, and Clinical Features of Brucellosis Patients Hospitalized in the North of Iran During 2009 - 2014

Brucellosis is a common zoonosis that is transmitted to humans through cattle, sheep, or goats (1). Brucellosis is widespread around the globe, especially in the Mediterranean region and the Middle East (2). Brucellosis is endemic in Iran with an annual incidence of 225 cases per hundred thousand people; in the Mazandaran province, it has been reported 114 cases per hundred thousand people (3). The disease is observed in farmers, herders, and veterinarians who have occupational exposure. The laboratory staff and other people who deal with cultures and infectious samples of dairy products and others who consume diaries, especially unpasteurized cheeses and milk, are at risk of the disease (1). The clinical manifestation of brucellosis is very diverse, mostly including nonspecific fever, night sweats, fatigue, myalgia, arthralgia, myalgia, weight loss, loss of appetite, and hepatosplenomegaly.

Given the high prevalence of brucellosis in our region and different clinical and laboratory manifestations in previous studies, this study was designed to determine the epidemiologic, laboratory, and clinical features of brucellosis in the north of Iran.

This was a cross-sectional study. The inclusion criteria included all patients who were hospitalized between 21 March 2009 and 20 March 2014 due to Brucella infection (according to the national protocol based on clinical evidence, the titre of 1:80 in Wright tests and 1:40 in 2ME positive) in Razi hospital of Qaemshahr city, the referral center for infectious diseases in Mazandaran province (north of Iran). The exclusion criteria included the absence of Brucella and the presence of kidney or liver disease.

The following criteria were defined: anemia as a hemoglobin level below 13 in men and 12 in women, leukopenia as a reduction in white blood cells (WBC) in circulation below 4500 cells/mcL, leukocytosis, as an increase in WBC in circulation over than 10,000 cells/mcL, thrombocytopenia as a reduction in platelet count below 150,000/mcL, hypokalemia as a potassium level below 3.3 mg/dL, hyperkalemia as a potassium level over 5.5 mg/dL, hyponatremia as a sodium level below 135 mg/dL, hypernatremia as a sodium level above 145 mg/dl, and the abnormal erythrocyte sedimentation rate (ESR) as ESR above age divided by two for men and age plus 10 divided by tow for women. Also, the creatinine level more than 1.5 mg/dL, AST < 5 or 40 < U/L, ALT < 7, or 56 < U/L, and ALP < 45 or < 115 U/L were considered abnormal (1). For most patients, a test was requested. Nevertheless, in cases where more than one test was requested, the most abnormal values were used.

We designed an information form to be used for collecting information and variables such as age, sex, clinical symptoms, and laboratory findings such as hemoglobin levels, WBC count, platelet count, potassium level, sodium level, ESR, AST, ALT, and ALP.

After collecting information, data were entered SPSS version 21 software and the descriptive analysis was run. Quantitative and qualitative values were examined using t-test and chi-square, respectively, and the significance level was set at a P value below 0.05.

In total, 219 patients with a mean age of 41.6 ± 16.9 (12 - 87) years were enrolled, among whom there were 86 females (39.27%) and 133 males (60.73%). The mean age was 46.3 ± 16.6 and 37.3 ± 17.2 for women and men, respectively (P < 0.001). 174 (79.4%) patients were suffering from non-focal brucellosis and 45 patients (20.6%) were diagnosed with focal brucellosis including 17 cases (37.8%) of spondylitis, 10 cases (22.2%) of arthritis, 7 cases (15.6%) of epididymo orchitis, 4 cases (8.9%) of sacroiliitis, 2 cases (4.4%) of neurobrucellosis, 2 cases (4.4%) of osteomyelitis, and 1 case (2.2%) of each of endocarditis, hepatitis, and thrombophlebitis of the lower extremities. Based on the statistical analysis, the type of detection in both sexes had no significant difference (P = 0.546).

The rates of referral per season were 23.7, 33.8, 23.3, and 19.2 percent in the spring, summer, autumn, and winter, respectively (P = 0.006). In terms of location of residence, 78 patients (35.6%) were living in cities and 141 patients (64.4%) in rural areas (P = 0.260). Of 219 participants, 85 (38.8%) mentioned animal contact. 191 (87.2%) patients had used local dairy products. In 20 participants (9.1%), including 15 non-focal and five focal brucellosis patients, the history of brucellosis in their family was positive.

The majority of clinical signs and symptoms included chills (78.5%), fever (58.4%), decreased appetite (44.7%), and sweating (42.5%) (Table 1). The highest chief complaints were fever and chills (31.1%), back pain (22.4%), myalgia cases (5.5%), inflation of Testis (2.7%), and fever and laminitis (1.8%).

Of 219 patients, 172 (78.6%) were hospitalized for 6 days or less and 47 (21.4%) for more than 6 days; in the latter group, there were 12 people with more than 12 days hospitalization period. About 70% of the patients were anemic. 88.2% of the patients had abnormal ALP (Table 2).

Although brucellosis is controlled in many developed countries, it remains a major problem for the health system in developing countries including the Mediterranean and the Middle East countries (4). In our study, 39% of the patients were female and 61% were male and the mean age was 41.6 ± 16.9; this study was similar to other studies in terms of age and gender of brucellosis patients (4-6).

Non-localized brucellosis was detected in 79.4% of patients in our study, while others were complicated with spondylitis, epididymo-orchitis, sacroiliitis, meningoencephalitis, osteomyelitis, arthritis, hepatitis, thrombophlebitis of the lower extremities, and endocarditis. In Roshan et al.’s study in 2004 in Babol, 31% of cases had a localized brucellosis (7). In a study by Najafi et al. in 2003 (8), about 8.7% of patients were complicated with epididymo orchitis while in this study, 3.2% of cases had this problem.

In our survey, 70% of patients were anemic. In addition, leukopenia and leukocytosis, each in 13%, thrombocytopenia in 4.1%, abnormal ESR in 56%, abnormal AST in 22%, abnormal ALT in 16%, and abnormal ALP in 88% of cases were observed. Various studies have presented different values. In a study by Karaman et al. in Turkey, anemia, leukopenia, and were reported in 28.6%, 13.9%, and 16% patients, respectively (9). Davoudi et al. reported a case of DVT in a 15-year-old boy with acute pain and swelling in his left thigh in June 2011, as a rare complication of Brucellosis (10). In a study by Fanni et al. in Tehran, anemia as 53%, leucopenia as 33%, and thrombocytopenia as 12% were reported (11). In an analysis conducted by Roushan et al. in Northern Iran, anemia in 15.1%, leucopenia in 3%, and abnormal ESR in 77.8% of patients were reported (7). In a study by Guler et al. in Turkey, leukopenia as 21.4%, anemia as 70%, thrombocytopenia as 23%, and pancytopenia were reported (12).

In our study, 23% of cases had jobs involving direct contact with animals and 87.2% had used local dairy products. Therefore, most of our patients had occupational exposure. In a study by Haddadi et al. in Tehran, 17.1% of patients had occupational exposure to livestock (13). In other studies, occupational exposure has been reported in 58.7%, 71%, 27%, and 32% of cases (7, 14). Therefore, although occupation is considered a risk factor, the disease is not necessarily transmitted occupationally in developing countries. However, the high percentage of using local dairy products both in our study and in other studies (15) suggests that the main route of transmission still remains the consumption of contaminated dairy products.

Most inflicted cases were observed in the spring and summer. Considering the spring and summer are the calving seasons with highest milk production in the cattle, the dairy production and contact with animals increase in this period. Thus, most of the cases were observed in the spring and summer. This is consistent with the findings of many previous studies (16, 17).

Most clinical signs and symptoms included chills, fever, loss of appetite, and sweating. In addition, the most common chief complaints included fever, chills, backache, and myalgia. In a 10-year clinical study on brucellosis patients in Macedonia, the most frequently observed symptoms were arthralgia, fever, and sweating and the most common signs were fever and hepatomegaly (18). In the Roushan’s study, the most common complaints were fever and arthralgia (7).

For serological diagnosis of human brucellosis, Rose Bengal, Wright’s Sero-agglutination, 2-ME, and antiglobulin Coombs tests are done as standard methods. Most patients with acute infection respond to all tests (19). In a study by Najafi et al. titled “Comparing the Serological Diagnostic value of ELISA and Wright tests in human brucellosis with positive PCR,” a clinical and laboratory study was conducted on 59 patients suspected of brucellosis, the Wright test compared to ELISA had higher sensitivity, lower specificity, approximately equal positive predictive value, higher negative predictive value, and generally higher accuracy (20).