Occurrence of Class 2 Integrons Among Multi-Drug Resistant Shigella Sonnei Isolated from Tehran, Iran in 2005

Reza Ranjbar; Shohreh Farshad; Mohammad Rahbar; Zahra Safiri; Caterina Mammina; Mohammad Arjomanzadegan

Archives of Clinical Infectious Diseases

Infectious Diseases and Tropical Medicine Research Center, SBUMS

Current Issue All Issues In Press Accepted Manuscripts Search

Indexing and Listing Sources Reviewer and AE Registration Form Journal Information Editors & Boards Journal Metrics Contact Us Open Peer Review (OPR)Publication Ethics and Malpractice Statement Support

APC

Authors Guide Submit Manuscript

Occurrence of Class 2 Integrons Among Multi-Drug Resistant Shigella Sonnei Isolated from Tehran, Iran in 2005

Author(s):

Reza Ranjbar

^1,*,

Shohreh Farshad²,

Mohammad Rahbar³,

Zahra Safiri¹,

Caterina Mammina⁴,

Mohammad Arjomanzadegan⁵

1Molecular Biology Research Center, Baqiyatallah University of Medical Sciences, Tehran, IR Iran

2Professor Alborzi Clinical Microbiology Research Center, Shiraz University of Medical Sciences, Shiraz, IR Iran

3Department of Microbiology, Iranian Reference Health Laboratories, Tehran, IR Iran

4Dipartimento di Scienze per la Promozione della Salute G. DAlessandro, Universit di Palermo, Via del Vespro, Palermo, Italy

5Research Center of Infectious Disease, Arak University of Medical Sciences, Arak, IR Iran

Archives of Clinical Infectious Diseases:Vol. 5, issue 3; 156-160

Article type:Brief Report

How to Cite:Reza RanjbarShohreh FarshadMohammad RahbarZahra SafiriCaterina MamminaMohammad Arjomanzadeganet al.Occurrence of Class 2 Integrons Among Multi-Drug Resistant Shigella Sonnei Isolated from Tehran, Iran in 2005.Arch Clin Infect Dis.5(3):156-160.

Abstract

Background:

Shigellosis is one of the major causes of morbidity in children with diarrhea in Iran. Integrons play an important role in the evolution and dissemination of multidrug resistance in gram-negative bacteria. The occurrence of integrons among Shigella spp. is frequently reported throughout the world. The aim of this study was to assess the occurrence of class 2 integrons among the multi drug resistant S. sonnei isolated from Iranian children in 2005.

Materials and methods:

The study was conducted in two major pediatric hospitals in Tehran, Iran. Fecal specimens and rectal swab collected from patients were cultured and identified as Shigella by the conventional methods. Antimicrobial susceptibility test was performed according to the standard CLSI guideline. Multi-drug resistant isolates of S. sonnei were further examined for the presence of class 2 integron by PCR using specific primers. Amplicons were

Results:

A total of 83 multi-drug resistant S. sonnei strains were isolated. Of these, 45 (54%) exhibited a class 2 integron of 2.1 kbp, and 34 (41%) a class 2 integron of 1.3 kbp. Class 2 integrons were not detected in four isolates.

Conclusion:

The results showed an increased occurrence of class 2 integron carrying S. sonnei isolated from children in Tehran in 2005.

Keywords

Shigella Sonnei

Class 2 Integron

Multi-Drug Resistant

Full Text

Full text is available in PDF

comments