Logo

Genetic Fingerprinting and Antimicrobial Susceptibility Profiles Pseudomonas aeruginosa Isolates From Eye Infections

Author(s):
Hossein GoudarziHossein Goudarzi1, Fatemeh KarimiFatemeh Karimi2, Fahimeh Asadi AmoliFahimeh Asadi Amoli3, Zohreh AbedinyfarZohreh Abedinyfar1, Farahnoosh DoustdarFarahnoosh Doustdar1, Faramarz MehrnejadFaramarz Mehrnejad2,*
1Department of Microbiology, Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran
2Department of Cellular and Molecular Biology, Faculty of Science, Azarbaijan University of Tarbiat Moalem, Tabriz, IR,Iran
3Eye Research Center, Farabi Hospital, Tehran University of Medical Science, Tehran, IR Iran


Archives of Clinical Infectious Diseases:Vol. 6, issue 1; 41-6
Article type:Research Article
How to Cite:Hossein GoudarziFatemeh KarimiFahimeh Asadi AmoliZohreh AbedinyfarFarahnoosh DoustdarFaramarz Mehrnejadet al.Genetic Fingerprinting and Antimicrobial Susceptibility Profiles Pseudomonas aeruginosa Isolates From Eye Infections.Arch Clin Infect Dis.6(1):41-6.

Abstract

Background:

As Pseudomonas aeruginosa is known the most common etiologic agent in microbial keratitis associated with contact lens use, this study was designed to study the distribution and patterns of resistance to antimicrobial agents of keratitis isolates in Iran. In this study, also the suitability of enterobacterial repetitive intergenic consensus (ERIC)-PCR to rapidly type P. aeruginosa strains isolated from patients with keratitis was examined.

Patients and Methods:

For this purpose, 57 clinically isolates of P. aeruginosa from keratitis patients referred to Farabi hospital were analyzed by antimicrobial susceptibility test using the disc diffusion method. Polymerase chain reaction with enterobacterial repetitive intergenic consensus primers (ERIC-PCR) was used to establish clonal relationship between the different isolates.

Results:

All the strains showed resistance to at least 4 antibiotics, but all were susceptible to fluoroquinolones. Multidrug resistance was found in two isolates (3.5%) which were resistant to more than one category of antibiotics including aminoglycoside (gentamicin) and ?-lactam (cefazoline). ERIC-PCR produced 53 different ERIC fingerprints, 49 of which contained only 1 strain. Eight of the isolates had 100% similarity, forming four real clones but considering 85% similarity cut off between isolates, 8 clones containing 25 isolates (43.8%) could be considered.

Conclusion:

Fluoroquinolones appeared to be the most effective agent against ocular P. aeruginosa isolates. Comparison of ERIC-PCR profiles revealed a low level of similarity among the strains analyzed. ERIC-PCR seems to be an inexpensive, fast, reproducible, and discriminatory DNA typing tool for effective epidemiologic surveillance of P. aeruginosa isolates potentially transmissible between patients with ocular infections.

Full Text

Full text is available in PDF

comments

Leave a comment here

Share on
Metrics

Purchasing Reprints

  • Copyright Clearance Center (CCC) handles bulk orders for article reprints for Brieflands. To place an order for reprints, please click here (   https://www.copyright.com/landing/reprintsinquiryform/ ). Clicking this link will bring you to a CCC request form where you can provide the details of your order. Once complete, please click the ‘Submit Request’ button and CCC’s Reprints Services team will generate a quote for your review.
Search Relations

Author(s):

Related Articles