Archives of Clinical Infectious Diseases
Urinary tract infection (UTI) is a frequently diagnosed renal and urologic disease. Escherichia coli is by far the most common etiologic agent of this disease. This study was aimed to type the E.coli strains isolated from the patients with urinary tract infection using sero-grouping. Detection of pap adhesion-encoding operon was also targeted.
A total of 130 E. coli strains isolated from patients with UTI were investigated for O-serotyping. The presence of pap adhesion-encoding operon was detected using polymerase chain reaction (PCR).
In serogrouping with 13 antisera, 86 strains (66.14%) were O-serogroupable and belonging to O1, O6, O15, O18 & O20 serogroups, while 44 strains (33.86%) were O-non typeable. Predominant serogroups were O6 and O18. The PCR results showed that 61% of strains exhibited the pap genotype. Serogroups O1, O6, O15 and O18 possessed pap operon. There was an obvious correlation between the pap operon and the O-serogroups of the strains.
Our results showed that obtained protein patterns of the isolated strains were more reliable than serotyping results for typing purposes. Our findings indicated that pap adhesionencoding operon has an important role in the development and severity of UTI. Many cases of serious urogenital diseases are caused by a limited number of uropathogenic E.coli strains that generally possess special virulence factors such as pap operon.
© 2009, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
Rahdar M, Rashki A, Miri HR, Rashki Ghalehnoo M. Detection of pap, sfa, afa, foc, and fim Adhesin-Encoding Operons in Uropathogenic Escherichia coli Isolates Collected From Patients With Urinary Tract Infection. Jundishapur J Microbiol. 2015;8(8):e22647. doi: https://doi.org/10.5812/jjm.22647
Rashki A, Rahdar M, Rashki Ghalehnoo Z. Characterization of Uropathogenic Escherichia coli: Distribution of Adhesin-Encoding Genes and O-Serotypes Among Ciprofloxacin Susceptible and Resistant Isolates. Jundishapur J Microbiol. 2019;12(9):e89179. doi: https://doi.org/10.5812/jjm.89179
Navidinia M, Najar Peerayeh S, Fallah F, Bakhshi B, Adabian S, et al. Distribution of the Pathogenicity Islands Markers (PAIs) in Uropathogenic E.coli Isolated from Children in Mofid Children Hospital. Arch Pediatr Infect Dis. 2013;1(2):. doi: https://doi.org/10.5812/pedinfect.9083
Rezaei MH, Kakian F, Gholipour A, Zamanzad B. The Frequency of PAI, aer and traT Genes in Escherichia coli Commensal and Urinary Pathogenic E. coli Isolates in Shahrekord and the Relationship Between the Two Groups by Multiplex PCR. Jundishapur J Microbiol. 2020;13(6):e98683. doi: https://doi.org/10.5812/jjm.98683
Inegol Paykoc E, Turkyilmaz S. Investigation of P Fimbriae Presence in Escherichia coli Strains Isolated from Urine Samples in Human, and Their Antibacterial Resistance. Jundishapur J Microbiol. 2018;11(9):e66119. doi: https://doi.org/10.5812/jjm.66119
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