Archives of Clinical Infectious Diseases
Gita Eslami1,*, Sudabe Taheri2, Sayed Abdol Majid Ayatollahi3, Ghazaleh Malek4, Fatemeh fallah1, Bita Pourkaveh1This research was carried out to compare antibacterial effect of Rosa nutkana sepal extract with synthetic antibiotics on methicillin resistant staphylococcus aureus (MRSA) bacteria isolated from patients with sty.
This descriptive study was done in 2009 at remedial center of Shahid Beheshti University and sampling was performed from patients suffering from sty using sterile soap. After preparation of Rosa nutkana sepal extract and after determining bacteria type, antibiogram test was used to determine bacterial resistance and sensitivity to cephalothin, co-amoxiclav, amoxicillin and tetracycline, as well as to the extract. Disc diffusion and agar dilution were used for this purpose. The area of non-growth resulting from antibiotic discs was compared with of the extract.
In this study, 31% of samples were reported to be MRSA. MRSA in 1/128 concentration of extract was sensitive in minimum inhibitory concentration (MIC) test. In disc diffusion test, Staphylococcus aureus was 100% sensitive to the extract, 15.8% to cephalothin, 26.3% to tetracycline, 31.6% to co-amoxiclav and 100% resistant to amoxicillin. Furthermore, there was not any microbial growth in agar dilution method (nutrient agar environment and 0.01% extract of plant sample). Moreover, there was not any microbial growth in plates containing Pseudomonas aeruginosa and Candida albicans.
Rosa nutkana sepal extract with antibiotics can be an important combination for treatment of sty infection.
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