Molecular Diagnosis of Tuberculosis: a New Primer Design

Syed Tasleem Raza; Gulraiz Ali; Sikandar Hasan; Farzana Mahdi

Archives of Clinical Infectious Diseases

Infectious Diseases and Tropical Medicine Research Center, SBUMS

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Molecular Diagnosis of Tuberculosis: a New Primer Design

Author(s):

Syed Tasleem Raza^1,*,

Gulraiz Ali¹,

Sikandar Hasan¹,

Farzana Mahdi¹

1Department of Biochemistry, ERA’S Lucknow Medical College, Lucknow, India

Archives of Clinical Infectious Diseases:Vol. 4, issue 2; e93400

Published online:Jul 30, 2009

Article type:Brief Report

Received:May 12, 2019

Accepted:Jul 21, 2007

How to Cite:Syed Tasleem RazaGulraiz AliSikandar HasanFarzana MahdiMolecular Diagnosis of Tuberculosis: a New Primer Design.Arch Clin Infect Dis.4(2):e93400.

Abstract

Background: Nucleic acid based detection of Mycobacterium tuberculosis has the potential to improve the analysis of the tuberculosis epidemiology and patient care by increasing the specificity and sensitivity of diagnosis.

Patients and methods: Fifty suspected cases were screened for M. tuberculosis by using a new primer sequence and comparing its results with the primer (IS6110) most widely used for tuberculosis diagnosis.

Results: The mean age of cases was 33.6±14.3 years. Twenty one cases (42%) were positive for M. tuberculosis. Maximum positivity was found in sputum (10/19) followed by menstrual blood (4/8), blood (5/15), ascitic fluid (1/2), and pleural fluid (1/5).

Conclusion: The new designed primer was reproducible and in complete concordance with the PCR-results obtained by the standard method using IS 6110 insertion sequence in all 50 cases in which both methods were used.

Keywords

Tuberculosis

Nucleic acid amplification

Molecular diagnosis

References

1.
The references are available in PDF file.

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