Coenzyme Q10 Supplementation and Oxidative Stress Parameters: An Updated Systematic Review and Meta-analysis of Randomized Controlled Clinical Trials

Alireza Sedaghat; Mohammad Samadi; Hossein Shirvani; Mojtaba Sepandi; Worya Tahmasebi

doi:10.5812/asjsm-131308

https://doi.org/10.5812/asjsm-131308

Coenzyme Q₁₀ Supplementation and Oxidative Stress Parameters: An Updated Systematic Review and Meta-analysis of Randomized Controlled Clinical Trials

authors:

Alireza Sedaghat ¹ , Mohammad Samadi ^{1
, *} , Hossein Shirvani ¹ , Mojtaba Sepandi ¹ , Worya Tahmasebi ²

1 Exercise Physiology Research Center, Life Style Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran

2 Department of Sports Nutrition, Faculty of Sports Sciences, Razi University, Kermanshah, Iran

Asian Journal of Sports Medicine: Vol.13, issue 3; e131308

published online: October 23, 2022

article type: Review Article

received: September 11, 2022

revised: October 15, 2022

accepted: October 15, 2022

how to cite: Sedaghat A, Samadi M, Shirvani H, Sepandi M, Tahmasebi W. Coenzyme Q₁₀ Supplementation and Oxidative Stress Parameters: An Updated Systematic Review and Meta-analysis of Randomized Controlled Clinical Trials. Asian J Sports Med. 2022;13(3):e131308. https://doi.org/10.5812/asjsm-131308.

Abstract

Background:

Oxidative stress (OS) contributes to the development of some disorders, including malignancies, metabolic diseases, Alzheimer's disease, and Parkinson's disease.

Objectives:

The effects of coenzyme Q₁₀ (CoQ₁₀) supplementation on OS parameters have been assessed through an updated systematic review and meta-analysis.

Methods:

Scopus, PubMed, Cochrane Library, EMBASE, and Web of Sciences were used for article searching. Standardized mean difference (SMD) and its standard error were calculated using a random-effects DerSimonian and Laird model. All analyses were done using the STATA software version 16.0 (StataCorp, College Station, TX).

Results:

Based on twenty-five studies which remained to be incorporated in the meta-analysis, a statistically significant decrease in malondialdehyde (MDA) (SMD -2.74; 95% CI -3.89, -1.58; I² = 96.9%) as well as nitric oxide (NO) (SMD -5.16; 95% CI -7.98, 2.34; I² = 92.5%) was associated with CoQ₁₀ supplementation, and a significant increase in total antioxidant capacity (TAC) (SMD 3.40; 95% CI 1.98, 4.83; I² = 97.4%) and superoxide dismutase (SOD) activity (SMD 1.22; 95% CI 0.32, 2.12; I² = 94.32%).

Conclusions:

The results showed no significant effect of CoQ₁₀ supplementation on glutathione peroxidase (GPx), catalase (CAT) activities, and glutathione (GSH) levels. Coenzyme Q₁₀ supplementation significantly reduced MDA and NO concentrations and increased TAC and SOD activity.

Keywords

Coenzyme Q₁₀ Oxidative Stress Superoxide Dismutase Malondialdehyde Total Antioxidant Capacity

1. Background

Extensive research shows that dietary antioxidants could protect body cells against free radicals. Ubiquinone, also known as coenzyme Q₁₀ (CoQ₁₀, 2,3-dimethoxy-5-methyl-6-decaprenyl benzoquinone), is an important endogenous cellular antioxidant (1). The human body and other living organisms, normal cellular metabolism, and environmental factors, such as air pollutants, can produce reactive oxygen species (ROS). This process which disturbs the balance between oxidants and antioxidants, is called oxidative stress (OS). The OS can cause damage to cell structures such as carbohydrates, nucleic acids, lipids, and proteins functions and contributes to the development of many diseases, such as cancer, diabetes, atherosclerosis, hypertension, chronic obstructive pulmonary disease, and so on (2, 3). In addition to the natural process of producing oxidative stress in the body, cells exercising with different intensities can induce oxidative stress, leading to fatigue, muscle damage, and impaired performance (4). Despite the many known health benefits of exercise, evidence shows that resistance and endurance exercise causes oxidative stress. During the mentioned activities, the body's oxygen consumption increases, which produces more ROS in the body. Since the amount of ROS produced exceeds the body's ability to detoxify them, some of them remain in the body and cause oxidative injury (5, 6). With the increase in aerobic exercise intensity, these damages will be increased (6). Numerous studies have shown the effect of coenzyme Q₁₀ on oxidative stress and its ability to scavenge against various free radicals (7-11). This supplement can reduce cellular oxidant activities in normal conditions, exercise-induced oxidative stress in damaged muscle, and improve various aspects of exercise performance at different intensities (4). Coenzyme Q₁₀ is poorly absorbed in electrons; as a result, it loses one or two electrons and is easily oxidized. The coenzyme Q₁₀ is a powerful antioxidant characterized by the rapid uptake and loss of electrons (12). Coenzyme Q₁₀ inhibits the production of free radicals (13). Coenzyme Q₁₀ regenerates vitamin E from α-tocopherol radicals (14). OS overproduction of reactive oxygen species is an important factor in developing diseases (ROS) (8). OS reveals an imbalance between ROS and the ability to repair the damage. Instabilities in the normal redox state of cells can cause toxic effects by producing free radicals that damage all components of the cell, including DNA. OS causes base damage and DNA strand breaks (15). The dietary composition can alter antioxidant mechanisms (16). Many randomized clinical trials (RCTs) and a few systematic reviews have investigated the impact of CoQ₁₀ on oxidative stress (8, 17-19), but the results of systematic reviews about the effect of CoQ₁₀ supplementation on oxidative stress seem to be inconclusive.

2. Objectives

The effects of coenzyme Q₁₀ (CoQ₁₀) supplementation on OS parameters were assessed through an updated systematic review and meta-analysis.

3. Methods

3.1. Search Strategy

MEDLINE, Web of Science, Scopus, Cochrane Library, and ClinicalTrials.gov were searched using the following keywords:

Glutathione Reductase OR Glutathione Peroxidase OR Superoxide Dismutase OR Nitric Oxide OR Oxidative Stress OR Malondialdehyde OR Total Antioxidant Capacity OR Total Antioxidant Status OR antioxidant OR Oxidant OR reactive oxygen species OR ROS OR Catalase OR reactive nitrogen species OR protein carbonyl) AND (Coenzyme Q₁₀ OR "Q₁₀" OR CoQ₁₀ OR Ubiquinone OR Bio-Quinone Q₁₀ OR Ubisemiquinone radical OR Ubisemiquinone OR Ubiquinol-10 OR Ubiquinol.

3.2. Inclusion and Exclusion Criteria

The independent reviewers selected two randomized placebo-controlled studies on adults (18 years or older) that evaluated the effects of CoQ₁₀ on malondialdehyde (MDA), glutathione (GSH), nitric oxide (NO) levels, glutathione peroxidase (GPx), catalase (CAT), superoxide dismutase (SOD) activities, and total antioxidant capacity (TAC), and were published between 2000 and 1st May 2022.

3.3. Data Extraction

Two authors performed data extraction and quality assessment of included studies independently. First author's name; type of participant disease; sample size; year of publication; the dose and duration of supplementation; age; body mass index (BMI); gender of participants; and outcome variables (CAT, TAC, MDA, GPx, GSH, NO, and SOD).

3.4. Assessment of Quality

The Cochrane risk of a bias assessment tool which assesses the adequacy of randomization sequence generation, allocation concealment, blinding, incomplete outcome data, attrition bias, and other potential sources of bias, was used for quality assessment.

3.5. Statistical Analysis and Data Synthesis

Firstly, the mean changes from the baseline to the end of the study period were extracted for each included study. In the second step, the correlation coefficient was calculated separately for the experimental and control groups as follows:

{C o r r}_{E} = \frac{{S D}_{E . b a s e l i n e}^{2} + {S D}_{E . f i n a l}^{2} - {S D}_{E . c h a n g e}^{2}}{2 \times {S D}_{E . b a s e l i n e} \times {S D}_{E . f i n a l}}

The SD changes from the baseline calculated using the correlation coefficient for each group separately as follow:

{S D}_{E . c h a n g e} = \sqrt{{S D}_{E . b a s e l i n e}^{2} + {S D}_{E . f i n a l}^{2} - (2 \times C o r r \times {S D}_{E . b a s e l i n e} \times {S D}_{E . f i n a l})}

Cochran's Q test and I² statistics were used to assess the heterogeneity between the studies. A high amount of I² (more than 75%) refers to the presence of heterogeneity. The random-effects DerSimonian and Laird model was used to calculate the pooled mean difference according to significant heterogeneity between studies. The publication bias was assessed by Begg's and Egger's tests. Moreover, the heterogeneity between the studies was determined via meta-regression analysis. The significance level for Begg's and Egger's tests was 0.1, and for other tests, 0.05 was considered a significant level. All analyses were done using STATA software version 16.0 (StataCorp, College Station, TX).

4. Results

4.1. Study Selection Process

Searching electronic databases led to 4158 records. Firstly, the found studies were screened using the title, abstract or full text. In this step, 274 articles were assessed for eligibility using the full text. Of these, 25 RCTs had inclusion criteria used for the final analysis. The screening steps of the studies are presented in Figure 1. The total of understudied cases in the studies was equal to 1169 people. The range of sample sizes varied from 20 to 100. Most of the studies were performed in Iran (16 papers). The doses of CoQ₁₀ prescribed in the included studies were from 30 to 400 mg per day, and the duration of supplement administration in different studies was between 1 and 24 weeks. More detail is shown in Table 1.

Figure 1.

PRISMA flow diagram of study selection

Table 1.

Main Characteristics of Included Studies

First Author	Country	Year	Population	Dose of Q₁₀	Sample Size	Duration (Weeks)	Outcome
Mohammadshahi et al. (19)	Iran	2014	Fatty liver disease patients	100 mg/kg /body weight	41	12	MDA↔
Singh et al. (18)	India	2018	Acute coronary syndrome	120 (mg/day)	55	12	TBARS↓, MDA↓
Sanoobar et al. (8)	Iran	2013	MS	500 (mg/day)	48	12	MDA, TAC, SOD, GTx
Zarei et al. (20)	Iran	2018	DM	100 (mg/day)	68	12	CAT↑, TAC↑
Jahangard et al. (21)	Iran	2019	Bipolar disorders	200 (mg/day)	69	8	↔MDA, ↔CAT, ↑TAC, ↓NO
Gholami et al. (22)	Iran	2018	T2DM	100 (mg/day)	68	12	MDA↓, 8-Isoprostane↓
Nattagh-Eshtivani et al. (23)	Iran	2018	Migraine	400 (mg/day)	46	12	MMP-9↓, NO↓
Liu et al. (24)	Taiwan	2015	Hepatocellular carcinoma	300 (mg/day)	39	12	MDA↑, SOD↑, CAT↑, GPx↑
Abdollahzad et al. (25)	Iran	2015	Rheumatoid arthritis	100 (mg/day)	44	8	TAC↔, MDA↓
Rodriguez-Carrizalez al. (26)	Mexic	2016	T2DM	400 (mg/day)	60	24	TAC↔, CAT↑, GTx↑
Raygan et al. (27)	Iran	2015	T2DM	100 (mg/day)	60	8	TAC↔, MDA↓, GSH↑
Moazen et al. (28)	Iran	2015	T2DM	100 (mg/day)	52	8	MDA↓
Akbari Fakhrabadi et al. (29)	Iran	2014	T2DM	200 (mg/day)	62	12	TAC↑
Carrasco et al. (30)	Spain	2014	Renal injury	200 (mg/day)	100	1	SOD↔, GPx ↔, GSH↔
Farhangi et al. (10)	Iran	2014	NAFLD	100 (mg/day)	41	4	MDA↓, TAC↑
Lee et al. (31)	Taiwan	2013	CAD	300 (mg/day)	42	12	SOD↑, CAT↑, GPx↑
Dai et al. (32)	Hong Kong	2011	Ventricular dysfunction	300 (mg/day)	56	8	SOD, 8-isoprostane↔
Gholnari et al. (11)	Iran	2018	Diabetic nephropathy	100 (mg/day)	50	12	MDA↓
Mousavinejad et al. (9)	Iran	2018	Autism	30 (mg/day)	52		MDA↓, SOD↓, GPx↓
Alahmar et al. (33)	Iraq	2020	Idiopathic infertility	200 (mg/day)	70	12	TAC↑, SOD↑, CAT↑
Hormozi et al. (34)	Iran	2019	Glazers with occupational cadmium exposure	120 (mg/day)	40	8	MDA↓, TAC↔, SOD↑, GPx↑ , CAT↓
Valizade Hasanloei et al. (35)	Iran	2021	ICU patients	400 (mg/day)	40	1	MDA↓
Sanders et al. (17)	USA	2020	Type II diabetes non-random	200 (mg/day)	14	2	MDA↓
Rostami and Jafari (36)	Iran	2010	Inactive men		20	2	TAC↓, MDA↔,
Ebrahimi et al. (37)	Iran	2019	MS women	300 (mg/day)	30	8	MDA↔, SOD↓, GPx↔, TAC↔

4.2. Effect of Coenzyme Q₁₀ Supplementation on Oxidative Stress Parameters

The MDA level was evaluated in 17 records. Coenzyme Q₁₀ significantly reduced MDA levels in the intervention group compared to the control group (standardized mean difference (SMD) - 2.74; 95% CI -3.89, -1.58; I² = 96.9%) (Figure 2). Results showed that CoQ₁₀ significantly reduced MDA levels in patients younger than 50 years (SMD -8.24; 95% CI -10.91, -5.57; I² = 97.5%). The intervention dose not significantly decrease MDA levels among patients older than 50 years (SMD -0.23; 95% CI -1.42, 0.95; I² = 96%). Results showed that in patients with BMI < 25, CoQ₁₀ supplementation significantly decreased MDA levels (SMD -7.53; 95% CI -10.87, -4.19; I² = 97. 5%). The intervention also significantly decreases MDA levels among patients with BMI ≥ 25 (SMD -1.55; 95% CI -2.37, -0.32; I² = 96.67%). According to different doses, CoQ₁₀ at a dose of less than 200 significantly reduced MDA levels (SMD -5.02; 95% CI -6.64, -3.40; I² = 97.2%). Also, CoQ₁₀ at a dose of more than 200 did not had significantly effect on MDA levels (SMD 0.37; 95% CI -1.47, 2.20; I² = 96.5%).

Effect of coenzyme Q10 supplementation on oxidative stress parameters

Figure 2.

Effect of coenzyme Q₁₀ supplementation on oxidative stress parameters

The effects of CoQ₁₀ supplementation on CAT activity were evaluated in seven records. Comparison to control group, CoQ₁₀ does not have significant effects on CAT activity (SMD 1.83; 95% CI -1.10, 4.76; I² = 98.3%). Also, different doses of CoQ₁₀ supplementation showed the same results, but the results were significant in patients with age > 50 and BMI > 25 (Figure 2).

The effects of CoQ₁₀ supplementation on GPx activity were evaluated in six records. According to pooled analysis, CoQ₁₀ does not have significant effects on GPx activity (SMD 1.01; 95% CI -0.97, 2.99; I² = 97.5%) (Figure 2). Two studies evaluated the effects of CoQ₁₀ supplementation on plasma NO. According to results, CoQ₁₀ supplementation significantly reduced plasma NO concentrations among intervention groups compared to the control group (SMD -5.16; 95% CI -7.98, -2.34; I² = 92.5%) (Figure 2).

The effect of CoQ₁₀ supplementation on SOD activity was assessed in nine records. According to a pooled analysis, CoQ₁₀ supplementation significantly increased SOD activity among the intervention groups (SMD 1.22; 95% CI 0.32, 2.12; I² = 94.32%) (Figure 2). According to a pooled analysis of 20 records, CoQ₁₀ supplementation significantly increased TAC levels among the intervention groups (SMD 3.40; 95% CI 1.98, 4.83; I² = 97.4%) (Figure 2). About the effects of CoQ₁₀ supplementation on GSH levels, the analysis was not showed a significant difference between intervention and placebo group (SMD 5.16; 95% CI -3.25, 13.57; I² = 99.2%) (Figure 2).

Table 2 shows each OS indexes overall and stratified pooled SMD estimation. Overall, the risk of bias among understudied papers was low. More than 70% of the included studies met the random sequence generation. However, allocation concealment and blinding were considered in more than 50% of studies (Figure 3).

Table 2.

The Effects of CoQ₁₀ Supplementation on Oxidative Stress with CIs 95% Using Subgroup Analysis

Variables	SMD	95% CI	T²	I² (%)
		MDA
Age
< 50	-8.24	-10.91, -5.57	12.14	97.5
≥ 50	-0.23	-1.42, 0.95	3.07	96
BMI
< 25	-7.53	-10.87, -4.19	11.9	97.5
≥ 25	-1.55	-2.78, -0.32	4.20	96.6
Dose
< 200	-5.02	-6.64, -3.04	6.2	97.3
≥ 200	0.37	-1.47, 2.20	4.9	96.5
Total	-2. 74	-3.89, -1.58	5.07	96.9
		CAT
Age
< 50	-1.95	-6.28, 2.38	10.3	98.2
≥ 50	6.64	0.31, 12.96	12.59	98.35
BMI
< 25	-48.56	-152.69, 55.57	558	98.8
≥ 25	3.13	0.10, 6.15	10.31	98.43
Dose
< 200	31.35	-169.59, 106.89	4451	99.3
≥ 200	-0.10	-2.19, 1.99	5.31	97.4
Total	1.83	-1.10, 4.76	12.6	98.3
		GPx
Age
< 50	3.91	-5.2, 13.0	62.6	98.5
≥ 50	0.37	-1.92, 2.66	3.96	97.0
BMI
< 25	4.67	-5.40, 14.75	77.11	98.64
≥ 25	-0.29	-1.67, 1.09	1.40	94.92
Dose
< 200	9.04	-12.6, 3.70	241	98.9
≥ 200	-0.07	-2.53, 1.14	3.2	96.3
Total	1.01	-0.97, 2.99	5.4	97.5
		SOD
Age
< 50	2.17	0.41, 3.92	3.14	96.4
≥ 50	0.70	-0.18, 1.58	0.7	88.25
BMI
< 25	4.45	1.6, 7.3	6.4	95.9
≥ 25	0.27	0.03, 0.52	1.62	94.3
Dose
< 200	34.23	-33.9, 102.4	1256	98.7
≥ 200	1.13	0.47, 1.80	0.7	88.7
Total	1.22	0.32, 2.12	1.57	94.3
		TAC
Age
< 50	2.23	0.57, 3.90	5.41	97.2
≥ 50	6.57	3.57, 9.58	8	97.5
BMI
< 25	3.19	-0.8, 7.2	11.7	97.9
≥ 25	3.48	2.05, 4.90	4.25	96.6
Dose
< 200	3.65	1.64, 5.65	5.7	96.9
≥ 200	3.49	1.17, 5.80	7.47	97.0
Total	3.40	1.98, 4.83	5.7	97.4
GSH	5.16	-3.25, 13.57	36.5	99.24
NO	-5.16	-7.98, -2.34	3.8	95.5

Figure 3.

Risk of bias graph across all included studies

4.3. Meta-regression Analysis

According to a simple meta-regression analysis, the study year and sample size do not significantly affect heterogeneity between studies about the understudied factors (P > 0.05).

4.4. Publication Bias

According to Begg's and Egger's tests, there was significant publication bias about the CAT, GPx, GSH, and NO. Nevertheless, Egger's test showed publication bias for MDA, SOD, and TAC (P = 0.01, 0.02, 0.003, respectively).

5. Discussion

The current study results are consistent with previous studies (8, 22, 38, 39), showing that supplementation with CoQ₁₀ significantly improves OS parameters. It increases TAC levels and SOD activities and decreases MDA and NO levels. However, this supplementation does not affect GSH or CAT levels. Also, the present study results, in line with other studies, showed that supplementation with CoQ₁₀ does not change GPx activity (40). There are few meta-analyses about the effects of CoQ₁₀ supplementation on OS profile. A meta-analysis by Akbari et al. showed a significant decrease in MDA (39). Another meta-analysis showed that the CoQ₁₀ supplementation increased SOD and CAT while decreasing MDA levels (38). According to our results, CoQ₁₀ supplementation significantly increases SOD in addition to the abovementioned factors. Antioxidant enzymes are the first defense barrier against ROS, and their reduction in the body will lead to increased OS (41). A study has shown that a daily intake of 150 mg of CoQ₁₀ supplementation significantly decreases oxidative stress and increases the activity of antioxidant enzymes (42).

CoQ₁₀ protects cells against apoptosis and OS damage (43). Apoptotic proteins like Bax lead to caspase-induced apoptosis by increasing cytochrome c release (38). On the other hand, anti-apoptotic proteins such as Bcl-2, by combining with Bax and reducing the release of cytochrome c, inhibit the apoptotic process (44). Caspase-3 can cause DNA fragmentation and, eventually, apoptosis (45, 46). However, this process was reversed by CoQ₁₀ (47).

Subgroup analyzes of our results show that CoQ₁₀ supplementation significantly increases TAC in overweight and obese individuals. Evidence suggests that obesity-related factors, including hyperglycemia, dyslipidemia, and increased adipose tissue, are important factors associated with oxidative stress (48).

In addition, our results showed that taking CoQ₁₀ at a dose of > 200 mg daily significantly changed TAC levels. Another study showed that higher doses of CoQ₁₀ may have faster and more stable antioxidant effects (42).

Our study results showed that CoQ₁₀ supplementation also significantly reduced MDA levels. MDA is a polyunsaturated fatty acids peroxidation product (49). Coenzyme Q₁₀ supplementation protects against lipid peroxidation, regulates lipid metabolism, and prevents lipid oxidation (50). Coenzyme Q₁₀ also limits the production of endogenous ROS in mitochondria (8, 51) and is usually related to decreased MDA levels (52). Coenzyme Q₁₀ is the main element of the antioxidant process in all parts of the cells (53) and can reduce ROS and free radicals levels, produced by the reaction with oxygen radicals or lipids through a direct reduction back to the tocopherol, resulting in improvement of oxidative stress (54). Q₁₀ supplementation may reduce plasma lipid peroxidation and regenerate tocopherol radicals (55).

Our systematic review and meta-analysis results, consistent with other studies (34, 56), show that CoQ₁₀ supplementation significantly affected SOD activity. Coenzyme Q₁₀ can play an antioxidant role by reducing ROS in mitochondria, thereby changing SOD activity (57). Despite a nonsignificant difference between intervention and placebo groups, the results of our study showed that CoQ₁₀supplementation changed CAT activity. The few primary studies may be why we could not find a significant effect of CoQ₁₀ supplementation on CAT activity. This study showed that CoQ₁₀ supplementation improves TAC and MDA concentrations and SOD and NO activity compared with placebo. Finally, CoQ₁₀, as a generally safe and well-tolerated supplement, could be considered for treating and preventing some human disorders. Furthermore, CoQ₁₀ can be used as an important adjuvant in treating different elderly diseases and chronic conditions.

5.1. Conclusions

CoQ₁₀ supplementation significantly reduces MDA and NO concentrations and increases TAC and SOD activity. However, the effects of CoQ₁₀ supplement on NO, GSH concentrations, or CAT activity were not statistically significant. It should be regarded that more interventional studies, such as clinical trials with higher sample sizes, are needed to assess the impact of CoQ₁₀ supplementation on oxidative stress parameters.

Footnotes

Authors' Contribution: A S, M S study concept and design. A S, M S , and M S participated in article searching and screening, performed the statistical analysis, and helped to draft the manuscript. H SH, W T participated in the article screening, manuscript writing, and revision. All authors read and approved the final manuscript.
Conflict of Interests: The authors clarify that this study does not have any funding support; also, they have no affiliations with or involvement in any organization or entity with any financial interest (such as honoraria; educational grants; membership, employment, consultancies, stock ownership, participation in speakers' bureaus, or other equity interest; and expert testimony or patent-licensing arrangements), or non-financial interest (such as personal or professional relationships, affiliations, knowledge or beliefs) in the subject matter or materials discussed in this manuscript. Also, the authors declare that this study did not have patents, and the authors do not have any relations with editorial board members or journal reviewers.
Data Reproducibility: No new data were created or analyzed in this study. Data sharing does not apply to this article.
Funding/Support: This study had no funding resources.

References

1.
Arumugam G, Manjula P, Paari N. A review: Anti diabetic medicinal plants used for diabetes mellitus. J Acute Dis. 2013;2(3):196-200. https://doi.org/10.1016/s2221-6189(13)60126-2.
2.
Birben E, Sahiner UM, Sackesen C, Erzurum S, Kalayci O. Oxidative stress and antioxidant defense. World Allergy Organ J. 2012;5(1):9-19. [PubMed ID: 23268465]. [PubMed Central ID: PMC3488923]. https://doi.org/10.1097/WOX.0b013e3182439613.
3.
Alzoghaibi MA. Concepts of oxidative stress and antioxidant defense in Crohn's disease. World J Gastroenterol. 2013;19(39):6540-7. [PubMed ID: 24151379]. [PubMed Central ID: PMC3801366]. https://doi.org/10.3748/wjg.v19.i39.6540.
4.
Ovchinnikov AN, Paoli A, Seleznev VV, Deryugina AV. Royal jelly plus coenzyme Q10 supplementation improves high-intensity interval exercise performance via changes in plasmatic and salivary biomarkers of oxidative stress and muscle damage in swimmers: a randomized, double-blind, placebo-controlled pilot trial. J Int Soc Sports Nutr. 2022;19(1):239-57. [PubMed ID: 35813842]. [PubMed Central ID: PMC9261740]. https://doi.org/10.1080/15502783.2022.2086015.
5.
Mastaloudis A, Leonard SW, Traber MG. Oxidative stress in athletes during extreme endurance exercise. Free Radic Biol Med. 2001;31(7):911-22. https://doi.org/10.1016/s0891-5849(01)00667-0.
6.
Feizi Y, Esmaeil Afzalpur M, Abtahi-Eivary SH. [Effect of 2-weeks Coenzyme Q10 Supplementation on Malondialdehyde and Catalase Serum Levels Following Moderate and Severe Acute Resistance Training in Inactive Female Students]. Intern Med Today. 2019;25(4):256-69. Persian. https://doi.org/10.32598/hms.25.4.256.
7.
Moradi M, Haghighatdoost F, Feizi A, Larijani B, Azadbakht L. Effect of Coenzyme Q10 Supplementation on Diabetes Biomarkers: a Systematic Review and Meta-analysis of Randomized Controlled Clinical Trials. Arch Iran Med. 2016;19(8):588-96. [PubMed ID: 27544369].
8.
Sanoobar M, Eghtesadi S, Azimi A, Khalili M, Jazayeri S, Reza Gohari M. Coenzyme Q10 supplementation reduces oxidative stress and increases antioxidant enzyme activity in patients with relapsing-remitting multiple sclerosis. Int J Neurosci. 2013;123(11):776-82. [PubMed ID: 23659338]. https://doi.org/10.3109/00207454.2013.801844.
9.
Mousavinejad E, Ghaffari MA, Riahi F, Hajmohammadi M, Tiznobeyk Z, Mousavinejad M. Coenzyme Q10 supplementation reduces oxidative stress and decreases antioxidant enzyme activity in children with autism spectrum disorders. Psychiatry Res. 2018;265:62-9. [PubMed ID: 29684771]. https://doi.org/10.1016/j.psychres.2018.03.061.
10.
Farhangi MA, Alipour B, Jafarvand E, Khoshbaten M. Oral coenzyme Q10 supplementation in patients with nonalcoholic fatty liver disease: effects on serum vaspin, chemerin, pentraxin 3, insulin resistance and oxidative stress. Arch Med Res. 2014;45(7):589-95. [PubMed ID: 25450583]. https://doi.org/10.1016/j.arcmed.2014.11.001.
11.
Gholnari T, Aghadavod E, Soleimani A, Hamidi GA, Sharifi N, Asemi Z. The Effects of Coenzyme Q10 Supplementation on Glucose Metabolism, Lipid Profiles, Inflammation, and Oxidative Stress in Patients With Diabetic Nephropathy: A Randomized, Double-Blind, Placebo-Controlled Trial. J Am Coll Nutr. 2018;37(3):188-93. [PubMed ID: 29111905]. https://doi.org/10.1080/07315724.2017.1386140.
12.
Duberley KE, Heales SJ, Abramov AY, Chalasani A, Land JM, Rahman S, et al. Effect of Coenzyme Q10 supplementation on mitochondrial electron transport chain activity and mitochondrial oxidative stress in Coenzyme Q10 deficient human neuronal cells. Int J Biochem Cell Biol. 2014;50:60-3. [PubMed ID: 24534273]. https://doi.org/10.1016/j.biocel.2014.02.003.
13.
Yalcin A, Kilinc E, Sagcan A, Kultursay H. Coenzyme Q10 concentrations in coronary artery disease. Clin Biochem. 2004;37(8):706-9. [PubMed ID: 15302616]. https://doi.org/10.1016/j.clinbiochem.2004.02.008.
14.
Kaikkonen J, Nyyssonen K, Tomasi A, Iannone A, Tuomainen TP, Porkkala-Sarataho E, et al. Antioxidative efficacy of parallel and combined supplementation with coenzyme Q10 and d-alpha-tocopherol in mildly hypercholesterolemic subjects: a randomized placebo-controlled clinical study. Free Radic Res. 2000;33(3):329-40. [PubMed ID: 10993487]. https://doi.org/10.1080/10715760000301501.
15.
Heshmati J, Golab F, Morvaridzadeh M, Potter E, Akbari-Fakhrabadi M, Farsi F, et al. The effects of curcumin supplementation on oxidative stress, Sirtuin-1 and peroxisome proliferator activated receptor gamma coactivator 1alpha gene expression in polycystic ovarian syndrome (PCOS) patients: A randomized placebo-controlled clinical trial. Diabetes Metab Syndr. 2020;14(2):77-82. [PubMed ID: 31991296]. https://doi.org/10.1016/j.dsx.2020.01.002.
16.
Sepidarkish M, Farsi F, Akbari-Fakhrabadi M, Namazi N, Almasi-Hashiani A, Maleki Hagiagha A, et al. The effect of vitamin D supplementation on oxidative stress parameters: A systematic review and meta-analysis of clinical trials. Pharmacol Res. 2019;139:141-52. [PubMed ID: 30447293]. https://doi.org/10.1016/j.phrs.2018.11.011.
17.
Sanders J, Smith S, Sanders C. Effect of CoQ10 Supplementation on Oxidative Stress and Muscle Bioenergetics in Type II Diabetes: A Pilot Study. Int J Diabetes Clin Res. 2020;7(1):116. https://doi.org/10.23937/2377-3634/1410116.
18.
Singh RB, Fedacko J, Mojto V, Pella D. Coenzyme Q10 Modulates Remodeling Possibly by Decreasing Angiotensin-Converting Enzyme in Patients with Acute Coronary Syndrome. Antioxidants (Basel). 2018;7(8):99. [PubMed ID: 30044377]. [PubMed Central ID: PMC6115761]. https://doi.org/10.3390/antiox7080099.
19.
Mohammadshahi M, Farsi F, Alavi Nejad P, Hajiani E, Zarei M, Ahmadi Engali K. The Coenzyme Q10 Supplementation Effects on Lipid Profile, Fasting Blood Sugar, Blood Pressure and Oxidative Stress Status Among Non-Alcoholic Fatty Liver Disease Patients: A Randomized, Placebo-Controlled, Pilot Study. J Gastroenterol Hepatol Res. 2014;3(6):1108-13.
20.
Zarei P, Rezvanfar MR, Ansarihadipour H, Delavar M, Abdollahi M, Khosrowbeygi A. Effects of coenzyme Q10 supplementation on the serum levels of amylase, adenosine deaminase, catalase, and total antioxidant capacity in women with type 2 diabetes mellitus: A randomized, double-blind placebo-controlled trial. J Res Med Sci. 2018;23:91. [PubMed ID: 30505329]. [PubMed Central ID: PMC6225443]. https://doi.org/10.4103/jrms.JRMS_970_17.
21.
Jahangard L, Yasrebifar F, Haghighi M, Ranjbar A, Mehrpooya M. Influence of adjuvant Coenzyme Q10 on inflammatory and oxidative stress biomarkers in patients with bipolar disorders during the depressive episode. Mol Biol Rep. 2019;46(5):5333-43. [PubMed ID: 31346916]. https://doi.org/10.1007/s11033-019-04989-z.
22.
Gholami M, Zarei P, Sadeghi Sedeh B, Rafiei F, Khosrowbeygi A. Effects of coenzyme Q10 supplementation on serum values of adiponectin, leptin, 8-isoprostane and malondialdehyde in women with type 2 diabetes. Gynecol Endocrinol. 2018;34(12):1059-63. [PubMed ID: 29933718]. https://doi.org/10.1080/09513590.2018.1481944.
23.
Nattagh-Eshtivani E, Dahri M, Hashemilar M, Tarighat-Esfanjani A. The effect of Coenzyme Q10 supplementation on serum levels of lactate, pyruvate, matrix metalloproteinase 9 and nitric oxide in women with migraine. A double blind, placebo, controlled randomized clinical trial. Eur J Integr Med. 2018;21:70-6. https://doi.org/10.1016/j.eujim.2018.06.009.
24.
Liu HT, Huang YC, Cheng SB, Huang YT, Lin PT. Effects of coenzyme Q10 supplementation on antioxidant capacity and inflammation in hepatocellular carcinoma patients after surgery: a randomized, placebo-controlled trial. Nutr J. 2015;15(1):85. [PubMed ID: 27716246]. [PubMed Central ID: PMC5053088]. https://doi.org/10.1186/s12937-016-0205-6.
25.
Abdollahzad H, Aghdashi MA, Asghari Jafarabadi M, Alipour B. Effects of Coenzyme Q10 Supplementation on Inflammatory Cytokines (TNF-alpha, IL-6) and Oxidative Stress in Rheumatoid Arthritis Patients: A Randomized Controlled Trial. Arch Med Res. 2015;46(7):527-33. [PubMed ID: 26342738]. https://doi.org/10.1016/j.arcmed.2015.08.006.
26.
Rodriguez-Carrizalez AD, Castellanos-Gonzalez JA, Martinez-Romero EC, Miller-Arrevillaga G, Pacheco-Moises FP, Roman-Pintos LM, et al. The effect of ubiquinone and combined antioxidant therapy on oxidative stress markers in non-proliferative diabetic retinopathy: A phase IIa, randomized, double-blind, and placebo-controlled study. Redox Rep. 2016;21(4):155-63. [PubMed ID: 26321469]. [PubMed Central ID: PMC8900707]. https://doi.org/10.1179/1351000215Y.0000000040.
27.
Raygan F, Rezavandi Z, Dadkhah Tehrani S, Farrokhian A, Asemi Z. The effects of coenzyme Q10 administration on glucose homeostasis parameters, lipid profiles, biomarkers of inflammation and oxidative stress in patients with metabolic syndrome. Eur J Nutr. 2016;55(8):2357-64. [PubMed ID: 26385228]. https://doi.org/10.1007/s00394-015-1042-7.
28.
Moazen M, Mazloom Z, Ahmadi A, Dabbaghmanesh MH, Roosta S. Effect of coenzyme Q10 on glycaemic control, oxidative stress and adiponectin in type 2 diabetes. J Pak Med Assoc. 2015;65(4):404-8. [PubMed ID: 25976576].
29.
Akbari Fakhrabadi M, Zeinali Ghotrom A, Mozaffari-Khosravi H, Hadi Nodoushan H, Nadjarzadeh A. Effect of Coenzyme Q10 on Oxidative Stress, Glycemic Control and Inflammation in Diabetic Neuropathy: A Double Blind Randomized Clinical Trial. Int J Vitam Nutr Res. 2014;84(5-6):252-60. [PubMed ID: 26255546]. https://doi.org/10.1024/0300-9831/a000211.
30.
Carrasco J, Anglada FJ, Campos JP, Muntane J, Requena MJ, Padillo J. The protective role of coenzyme Q10 in renal injury associated with extracorporeal shockwave lithotripsy: a randomised, placebo-controlled clinical trial. BJU Int. 2014;113(6):942-50. [PubMed ID: 24119199]. https://doi.org/10.1111/bju.12485.
31.
Lee BJ, Tseng YF, Yen CH, Lin PT. Effects of coenzyme Q10 supplementation (300 mg/day) on antioxidation and anti-inflammation in coronary artery disease patients during statins therapy: a randomized, placebo-controlled trial. Nutr J. 2013;12(1):142. [PubMed ID: 24192015]. [PubMed Central ID: PMC4176102]. https://doi.org/10.1186/1475-2891-12-142.
32.
Dai YL, Luk TH, Yiu KH, Wang M, Yip PM, Lee SW, et al. Reversal of mitochondrial dysfunction by coenzyme Q10 supplement improves endothelial function in patients with ischaemic left ventricular systolic dysfunction: a randomized controlled trial. Atherosclerosis. 2011;216(2):395-401. [PubMed ID: 21388622]. https://doi.org/10.1016/j.atherosclerosis.2011.02.013.
33.
Alahmar AT, Calogero AE, Sengupta P, Dutta S. Coenzyme Q10 Improves Sperm Parameters, Oxidative Stress Markers and Sperm DNA Fragmentation in Infertile Patients with Idiopathic Oligoasthenozoospermia. World J Mens Health. 2021;39(2):346-51. [PubMed ID: 32009311]. [PubMed Central ID: PMC7994657]. https://doi.org/10.5534/wjmh.190145.
34.
Hormozi M, Mirzaei R, Nakhaee A, Payandeh A, Izadi S, Haghighi JD. Effects of coenzyme Q10 supplementation on oxidative stress and antioxidant enzyme activity in glazers with occupational cadmium exposure: A randomized, double-blind, placebo-controlled crossover clinical trial. Toxicol Ind Health. 2019;35(1):32-42. [PubMed ID: 30453844]. https://doi.org/10.1177/0748233718809256.
35.
Valizade Hasanloei MA, Zeinaly A, Rahimlou M, Houshyar H, Moonesirad S, Hashemi R. Effect of coenzyme Q10 supplementation on oxidative stress and clinical outcomes in patients with low levels of coenzyme Q10 admitted to the intensive care unit. J Nutr Sci. 2021;10. e48. [PubMed ID: 34290862]. [PubMed Central ID: PMC8278158]. https://doi.org/10.1017/jns.2021.39.
36.
Rostami AR, Jafari A. [Effect of Short-term Coenzyme Q10 Supplementation on Oxidative Stress Index and Total Antioxidant Capacity of Serum in Inactive Men]. Med J Tabriz Uni Med Sciences Health Services. 2012;34(3):46-51. Persian.
37.
Ebrahimi S, Haghighi AH, Nikkhah K, Hamidi H. [Effects of Coenzyme Q10 Supplementation on Antioxidant Enzyme and Lipid peroxide Activities in Women with Multiple Sclerosis: a Randomized, Placebo-controlled Trial]. Iranian J Nutr Sci Food Technol. 2019;14(3):11-20. Persian.
38.
Jorat MV, Tabrizi R, Kolahdooz F, Akbari M, Salami M, Heydari ST, et al. The effects of coenzyme Q10 supplementation on biomarkers of inflammation and oxidative stress in among coronary artery disease: a systematic review and meta-analysis of randomized controlled trials. Inflammopharmacology. 2019;27(2):233-48. [PubMed ID: 30758695]. https://doi.org/10.1007/s10787-019-00572-x.
39.
Akbari A, Mobini GR, Agah S, Morvaridzadeh M, Omidi A, Potter E, et al. Coenzyme Q10 supplementation and oxidative stress parameters: a systematic review and meta-analysis of clinical trials. Eur J Clin Pharmacol. 2020;76(11):1483-99. [PubMed ID: 32583356]. https://doi.org/10.1007/s00228-020-02919-8.
40.
Dlugosz A, Kuzniar J, Sawicka E, Marchewka Z, Lembas-Bogaczyk J, Sajewicz W, et al. Oxidative stress and coenzyme Q10 supplementation in renal transplant recipients. Int Urol Nephrol. 2004;36(2):253-8. [PubMed ID: 15368706]. https://doi.org/10.1023/b:urol.0000034652.88578.a8.
41.
Bocci V, Valacchi G. Free radicals and antioxidants: how to reestablish redox homeostasis in chronic diseases? Curr Med Chem. 2013;20(27):3397-415. [PubMed ID: 23590717]. https://doi.org/10.2174/0929867311320270005.
42.
Lee BJ, Huang YC, Chen SJ, Lin PT. Coenzyme Q10 supplementation reduces oxidative stress and increases antioxidant enzyme activity in patients with coronary artery disease. Nutrition. 2012;28(3):250-5. [PubMed ID: 21996047]. https://doi.org/10.1016/j.nut.2011.06.004.
43.
Stocker R, Bowry VW, Frei B. Ubiquinol-10 protects human low density lipoprotein more efficiently against lipid peroxidation than does alpha-tocopherol. Proc Natl Acad Sci U S A. 1991;88(5):1646-50. [PubMed ID: 2000375]. [PubMed Central ID: PMC51081]. https://doi.org/10.1073/pnas.88.5.1646.
44.
Chen M, Tan M, Jing M, Liu A, Liu Q, Wen S, et al. Berberine protects homocysteic acid-induced HT-22 cell death: involvement of Akt pathway. Metab Brain Dis. 2015;30(1):137-42. [PubMed ID: 25048007]. https://doi.org/10.1007/s11011-014-9580-x.
45.
Slee EA, Harte MT, Kluck RM, Wolf BB, Casiano CA, Newmeyer DD, et al. Ordering the cytochrome c-initiated caspase cascade: hierarchical activation of caspases-2, -3, -6, -7, -8, and -10 in a caspase-9-dependent manner. J Cell Biol. 1999;144(2):281-92. [PubMed ID: 9922454]. [PubMed Central ID: PMC2132895]. https://doi.org/10.1083/jcb.144.2.281.
46.
Kasai H, Yamamoto K, Koseki T, Yokota M, Nishihara T. Involvement of caspase activation through release of cytochrome c from mitochondria in apoptotic cell death of macrophages infected with Actinobacillus actinomycetemcomitans. FEMS Microbiol Lett. 2004;233(1):29-35. [PubMed ID: 15043866]. https://doi.org/10.1016/j.femsle.2004.01.034.
47.
Li X, Zhan J, Hou Y, Hou Y, Chen S, Luo D, et al. Coenzyme Q10 Regulation of Apoptosis and Oxidative Stress in H2O2 Induced BMSC Death by Modulating the Nrf-2/NQO-1 Signaling Pathway and Its Application in a Model of Spinal Cord Injury. Oxid Med Cell Longev. 2019;2019:6493081. [PubMed ID: 31915512]. [PubMed Central ID: PMC6930770]. https://doi.org/10.1155/2019/6493081.
48.
Vincent HK, Taylor AG. Biomarkers and potential mechanisms of obesity-induced oxidant stress in humans. Int J Obes (Lond). 2006;30(3):400-18. [PubMed ID: 16302012]. https://doi.org/10.1038/sj.ijo.0803177.
49.
Heshmati J, Farsi F, Shokri F, Rezaeinejad M, Almasi-Hashiani A, Vesali S, et al. A systematic review and meta-analysis of the probiotics and synbiotics effects on oxidative stress. J Funct Foods. 2018;46:66-84. https://doi.org/10.1016/j.jff.2018.04.049.
50.
Talevi R, Barbato V, Fiorentino I, Braun S, Longobardi S, Gualtieri R. Protective effects of in vitro treatment with zinc, d-aspartate and coenzyme q10 on human sperm motility, lipid peroxidation and DNA fragmentation. Reprod Biol Endocrinol. 2013;11:81. [PubMed ID: 23958080]. [PubMed Central ID: PMC3765367]. https://doi.org/10.1186/1477-7827-11-81.
51.
Jing L, He MT, Chang Y, Mehta SL, He QP, Zhang JZ, et al. Coenzyme Q10 protects astrocytes from ROS-induced damage through inhibition of mitochondria-mediated cell death pathway. Int J Biol Sci. 2015;11(1):59-66. [PubMed ID: 25552930]. [PubMed Central ID: PMC4278255]. https://doi.org/10.7150/ijbs.10174.
52.
Karajibani M, Hashemi M, Montazerifar F, Bolouri A, Dikshit M. The status of glutathione peroxidase, superoxide dismutase, vitamins A, C, E and malondialdehyde in patients with cardiovascular disease in Zahedan, Southeast Iran. J Nutr Sci Vitaminol (Tokyo). 2009;55(4):309-16. [PubMed ID: 19763031]. https://doi.org/10.3177/jnsv.55.309.
53.
Crane FL. Biochemical functions of coenzyme Q10. J Am Coll Nutr. 2001;20(6):591-8. [PubMed ID: 11771674]. https://doi.org/10.1080/07315724.2001.10719063.
54.
Arroyo A, Kagan VE, Tyurin VA, Burgess JR, de Cabo R, Navas P, et al. NADH and NADPH-dependent reduction of coenzyme Q at the plasma membrane. Antioxid Redox Signal. 2000;2(2):251-62. [PubMed ID: 11229530]. https://doi.org/10.1089/ars.2000.2.2-251.
55.
Kaikkonen J, Tuomainen TP, Nyyssonen K, Salonen JT. Coenzyme Q10: absorption, antioxidative properties, determinants, and plasma levels. Free Radic Res. 2002;36(4):389-97. [PubMed ID: 12069102]. https://doi.org/10.1080/10715760290021234.
56.
Tiano L, Belardinelli R, Carnevali P, Principi F, Seddaiu G, Littarru GP. Effect of coenzyme Q10 administration on endothelial function and extracellular superoxide dismutase in patients with ischaemic heart disease: a double-blind, randomized controlled study. Eur Heart J. 2007;28(18):2249-55. [PubMed ID: 17644511]. https://doi.org/10.1093/eurheartj/ehm267.
57.
Sourris KC, Harcourt BE, Tang PH, Morley AL, Huynh K, Penfold SA, et al. Ubiquinone (coenzyme Q10) prevents renal mitochondrial dysfunction in an experimental model of type 2 diabetes. Free Radic Biol Med. 2012;52(3):716-23. [PubMed ID: 22172526]. https://doi.org/10.1016/j.freeradbiomed.2011.11.017.

comments