Investigation of Ulcerative Colitis for Herpes Simplex Virus and Cytomegalovirus Genomic Sequences by the Polymerase Chain Reaction


avatar Sahar Mehrabani-Khasraghi 1 , * , avatar Mitra Ameli 2 , avatar Farzad Khalily 3

Department of Microbiology, Tonekabon Branch, Islamic Azad University, Tonekabon, IR Iran
Department of Medicine, Tonekabon Branch, Islamic Azad University, Tonekabon, IR Iran
Gastroenterology and Hepatology Research Center, Alborz University of Medical Sciences, Karaj, IR Iran

how to cite: Mehrabani-Khasraghi S, Ameli M, Khalily F. Investigation of Ulcerative Colitis for Herpes Simplex Virus and Cytomegalovirus Genomic Sequences by the Polymerase Chain Reaction. Gene Cell Tissue. 2015;2(4):e32846.



  • 1.

    Baumgart DC, Carding SR. Inflammatory bowel disease: cause and immunobiology. The Lancet. 2007;369(9573):1627-40.

  • 2.

    Baumgart DC, Sandborn WJ. Inflammatory bowel disease: clinical aspects and established and evolving therapies. Lancet. 2007;369(9573):1641-57. [PubMed ID: 17499606].

  • 3.

    Xavier RJ, Podolsky DK. Unravelling the pathogenesis of inflammatory bowel disease. Nature. 2007;448(7152):427-34. [PubMed ID: 17653185].

  • 4.

    Danese S, Fiocchi C. Ulcerative colitis. N Engl J Med. 2011;365(18):1713-25. [PubMed ID: 22047562].

  • 5.

    Garland CF, Lilienfeld AM, Mendeloff AI, Markowitz JA, Terrell KB, Garland FC. Incidence rates of ulcerative colitis and Crohn's disease in fifteen areas of the United States. Gastroenterology. 1981;81(6):1115-24. [PubMed ID: 7286589].

  • 6.

    Bertalot G, Villanacci V, Gramegna M, Orvieto E, Negrini R, Saleri A, et al. Evidence of Epstein-Barr virus infection in ulcerative colitis. Dig Liver Dis. 2001;33(7):551-8. [PubMed ID: 11816543].

  • 7.

    Kangro HO, Chong SK, Hardiman A, Heath RB, Walker-Smith JA. A prospective study of viral and mycoplasma infections in chronic inflammatory bowel disease. Gastroenterology. 1990;98(3):549-53. [PubMed ID: 2298361].

  • 8.

    Vega R, Bertran X, Menacho M, Domenech E, Moreno de Vega V, Hombrados M, et al. Cytomegalovirus infection in patients with inflammatory bowel disease. Am J Gastroenterol. 1999;94(4):1053-6. [PubMed ID: 10201482].

  • 9.

    Hamlin PJ, Shah MN, Scott N, Wyatt JI, Howdle PD. Systemic cytomegalovirus infection complicating ulcerative colitis: a case report and review of the literature. Postgrad Med J. 2004;80(942):233-5. [PubMed ID: 15082847].

  • 10.

    de Saussure P, Lavergne-Slove A, Mazeron MC, Alain S, Matuchansky C, Bouhnik Y. A prospective assessment of cytomegalovirus infection in active inflammatory bowel disease. Aliment Pharmacol Ther. 2004;20(11-12):1323-7. [PubMed ID: 15606394].

  • 11.

    Kandiel A, Lashner B. Cytomegalovirus colitis complicating inflammatory bowel disease. Am J Gastroenterol. 2006;101(12):2857-65. [PubMed ID: 17026558].

  • 12.

    Powell RD, Warner NE, Levine RS, Kirsner JB. Cytomegalic inclusion disease and ulcerative colitis; report of a case in a young adult. Am J Med. 1961;30:334-40. [PubMed ID: 13737621].

  • 13.

    Orvar K, Murray J, Carmen G, Conklin J. Cytomegalovirus infection associated with onset of inflammatory bowel disease. Dig Dis Sci. 1993;38(12):2307-10. [PubMed ID: 8261839].

  • 14.

    Yi F, Zhao J, Luckheeram RV, Lei Y, Wang C, Huang S, et al. The prevalence and risk factors of cytomegalovirus infection in inflammatory bowel disease in Wuhan, Central China. Virol J. 2013;10:43. [PubMed ID: 23374225].

  • 15.

    Dimitroulia E, Pitiriga VC, Piperaki ET, Spanakis NE, Tsakris A. Inflammatory bowel disease exacerbation associated with Epstein-Barr virus infection. Dis Colon Rectum. 2013;56(3):322-7. [PubMed ID: 23392146].

  • 16.

    Kishore J, Ghoshal U, Ghoshal UC, Krishnani N, Kumar S, Singh M, et al. Infection with cytomegalovirus in patients with inflammatory bowel disease: prevalence, clinical significance and outcome. J Med Microbiol. 2004;53(Pt 11):1155-60. [PubMed ID: 15496396].

  • 17.

    Gehlert T, Devergne O, Niedobitek G. Epstein-Barr virus (EBV) infection and expression of the interleukin-12 family member EBV-induced gene 3 (EBI3) in chronic inflammatory bowel disease. J Med Virol. 2004;73(3):432-8. [PubMed ID: 15170639].

  • 18.

    Spieker T, Herbst H. Distribution and phenotype of Epstein-Barr virus-infected cells in inflammatory bowel disease. Am J Pathol. 2000;157(1):51-7. [PubMed ID: 10880375].

  • 19.

    Yanai H, Shimizu N, Nagasaki S, Mitani N, Okita K. Epstein-Barr virus infection of the colon with inflammatory bowel disease. Am J Gastroenterol. 1999;94(6):1582-6. [PubMed ID: 10364028].

  • 20.

    Wakefield AJ, Fox JD, Sawyerr AM, Taylor JE, Sweenie CH, Smith M, et al. Detection of herpesvirus DNA in the large intestine of patients with ulcerative colitis and Crohn's disease using the nested polymerase chain reaction. J Med Virol. 1992;38(3):183-90. [PubMed ID: 1287131].

  • 21.

    Hommes DW, Sterringa G, van Deventer SJ, Tytgat GN, Weel J. The pathogenicity of cytomegalovirus in inflammatory bowel disease: a systematic review and evidence-based recommendations for future research. Inflamm Bowel Dis. 2004;10(3):245-50. [PubMed ID: 15290919].

  • 22.

    Leveque N, Brixi-Benmansour H, Reig T, Renois F, Talmud D, Brodard V, et al. Low frequency of cytomegalovirus infection during exacerbations of inflammatory bowel diseases. J Med Virol. 2010;82(10):1694-700. [PubMed ID: 20827767].

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