https://doi.org/10.5812/hepatmon.111617

COVID-19 Among Patients With Hepatitis B or Hepatitis C: A Systematic Review

authors:

avatar Hossein Mirzaie ORCID 1 , avatar Mohammad Vahidi 2 , avatar Mostafa Shokoohi ORCID 1 , 3 , avatar Maryam Darvishian 4 , avatar Hamid Sharifi ORCID 1 , avatar Heidar Sharafi ORCID 5 , avatar Mohammad Karamouzian ORCID 6 , *

HIV/STI Surveillance Research Center, and WHO Collaborating Center for HIV Surveillance, Institute for Futures Studies in Health, Kerman University of Medical Sciences, Kerman, Iran
Faculty of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
Dalla Lana School of Public Health, University of Toronto, Toronto, ON, Canada
Cancer Control Research, BC Cancer Research Centre, Vancouver, BC, Canada
Middle East Liver Diseases (MELD) Center, Tehran, Iran
School of Population and Public Health, Faculty of Medicine, University of British Columbia, Vancouver, BC, Canada
Hepatitis Monthly: Vol.20, issue 11; e111617
published online: January 9, 2021
article type: Systematic Review
received: November 25, 2020
revised: December 19, 2020
accepted: December 29, 2020

how to cite: Mirzaie H, Vahidi M, Shokoohi M, Darvishian M, Sharifi H, et al. COVID-19 Among Patients With Hepatitis B or Hepatitis C: A Systematic Review. Hepat Mon. 2020;20(11):e111617. https://doi.org/10.5812/hepatmon.111617.

Abstract

Context:

Hepatic manifestations of Coronavirus Disease 2019 (COVID-19) are common among people living with Hepatitis B Virus (HBV) and Hepatitis C Virus (HCV).

Objectives:

This systematic review aimed to summarize the evidence on COVID-19 patients living with HBV or HCV co-infections.

Data Sources:

We searched multiple electronic databases and preprint servers from December 1, 2019, to August 9, 2020.

Study Selection:

Studies were included if they reported quantitative empirical data on COVID-19 patients living with HBV or HCV co-infections.

Data Extraction:

Descriptive analyses were reported, and data were synthesized narratively. The quality assessment was completed using the Joanna Briggs Institute critical appraisal tools.

Results:

Out of the 941 uniquely identified records, 27 studies were included. Of the eligible studies, 232 COVID-19 patients were living with HBV and 22 were living with HCV. Most patients were male, and the mean age was 49.8 and 62.8 years in patients living with HBV and HCV, respectively. Among the reported cases of SARS-CoV-2-HBV co-infection, the proportions of death were 4.7% and 15% in cross-sectional and case series/report studies, respectively. The death proportion was 8.3% among the reported cases of SARS-CoV-2-HCV co-infection. Among COVID-19 patients, 34.1% and 76.2% reported at least one comorbidity besides HBV and HCV infections, mainly hypertension and type 2 diabetes mellitus. The most common COVID-19-related symptoms in both HBV and HCV groups were fever, cough, dyspnea, fatigue, and gastrointestinal symptoms.

Conclusions:

While understanding the pathogenesis of SARS-CoV-2 requires further investigations, the careful assessment of hepatic manifestations and chronic infections, such as HBV and HCV upon the admission of COVID-19 patients could help reduce multimorbidity among HBV or HCV patients and lead to more favorable health outcomes among them.

1. Context

The first case of Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) infection was reported in China in December 2019 (1). The World Health Organization (WHO) declared Coronavirus Disease 2019 (COVID-19) a pandemic on March 11, 2020. As of October 19, 2020, > 48 million cases and > 1,200,000 deaths were reported (2). A more severe disease course and a higher mortality rate have been reported in elderly patients, as well as those with underlying conditions, including hypertension, asthma, diabetes mellitus, chronic lung disease, cardiovascular conditions, obesity, and chronic kidney disease (3-8). COVID-19 clinical presentations could vary greatly but often include respiratory, gastrointestinal, renal, and neurological manifestations (9, 10).

Recent studies have reported COVID-19 patients presenting with acute hepatitis before demonstrating respiratory symptoms (11). While our understanding of SARS-CoV-2 pathogenesis continues to grow, initial studies suggest that the virus could lead to liver injury mainly by binding to Angiotensin-Converting Enzyme 2 (ACE2) receptors on hepatocytes or causing an immune-mediated hepatic injury through cytokine storm activation (12-14). Several studies suggest that COVID-19 could lead to liver injuries and elevated Alanine Aminotransferase (ALT), Aspartate Aminotransferase (AST), and total bilirubin, particularly among severe COVID-19 cases (e.g., those admitted to Intensive Care Unit [ICU]) (15-18). Abnormal liver functions among COVID-19 patients have also been associated with increased disease severity and risk of mortality (19, 20). A recent meta-analysis of a few studies on the hepatic manifestations of COVID-19 estimated the pooled prevalence of pre-existing chronic liver disease at 1.9%, pre-existing liver cirrhosis at 0.4%, HBV at 0.9%, and HCV at 0.3% (15). While insightful, these findings are limited, as they are only based on less than a handful of small sample-sized studies conducted in China and the USA during the early months of the pandemic (i.e., as late as April 2020) (15). These findings also contradict other earlier studies that reported a significantly higher prevalence of liver complications among COVID-19 patients in China, where 2-11% of COVID-19 patients had liver comorbidities and up to 54% had elevated AST and ALT levels (21).

COVID-19-related hepatic complications are of concern, particularly among people living with HCV, HBV, or HBV/HCV co-infection with pre-existing liver complications (e.g., cirrhosis, liver failure, hepatocellular carcinoma) (15, 21, 22). Considering that around 290 million and 71 million people are living with HBV and HCV, respectively (23, 24), the number of patients with SARS-CoV-2 and HBV or HCV co-infection is likely to increase. Therefore, improving our understanding of the hepatic manifestations and comorbidities among COVID-19 patients living with HBV, HCV, or both is of utmost importance in enhancing the care provided for this large at-risk population.

2. Objectives

In this systematic review, we aimed to review and summarize the existing literature on COVID-19 patients living with HBV or HCV comorbidities. The findings of our review could provide information on clinical care and treatment options for COVID-19 patients with these co-infections.

3. Data Sources

The inclusion criteria and analytical plan were conceptualized a priori and are documented in the Open Science Framework ( https://osf.io/p4w3j/).

Following the Systematic Reviews and Meta-Analyses (PRISMA) and Peer Review of Electronic Search Strategies (25) guidelines (see Supplementary File S1 for PRISMA checklist), we searched PubMed, Scopus, Web of Science, CINAHL, Embase, and Google Scholar, as well as preprint databases, including medRxiv and bioRxiv from December 1, 2019, to August 9, 2020. Search terms were combined using appropriate Boolean operators and included subject heading terms/keywords relevant to COVID-19 (e.g., SARS-CoV-2 or coronavirus disease 2019 or COVID-19 or severe acute respiratory syndrome coronavirus 2 or coronavirus infection) and hepatitis B or C (e.g., liver fibrosis or liver cirrhosis or hepatic transplantation or liver transplant or hepatitis C or hepatitis B or HBV or HCV). Please see Supplementary File S2 for our sample search strategy.

4. Study Selection

Quantitative studies of any type (i.e., case report, case series, cross-sectional, case-control, cohort, and clinical trial) that reported individual-level and/or aggregate-level data on COVID-19 patients living with HBV, HCV, or HBV-HCV-co-infection were included in this review. Studies that combined the samples of HBV/HCV-positive and HBV/HCV-negative patients were only included if they provided subgroup analyses for people living with HBV, HCV, or HBV-HCV-co-infection. Studies were excluded if they did not present original empirical data or did not report any clinical data for patients. Two reviewers (HM and MV) independently screened all titles, abstracts, and full-texts. Duplicate records were excluded, and disagreements were resolved by discussion or arbitration by the senior author (MK).

5. Data Extraction

Data were extracted on a) Study characteristics, including first author, study period, study design, sample size, data type, and study population, b) Sociodemographic characteristics, such as patients' age and sex/gender, c) HBV- and HCV-related characteristics, including cirrhosis status, liver transplantation, received immunosuppressive therapy, and liver enzyme levels (e.g., AST, ALT), d) COVID-19-related characteristics, including symptoms and severity of COVID-19 defined as mild (i.e., no or mild pneumonia), severe (i.e., blood oxygen saturation ≤ 93%, dyspnea, or lung infiltrates > 50% within two days), or critical (i.e., septic shock, respiratory failure, or multiple organ failures) (26). Hospitalization, ICU admission, survival status (recovery or death), and non-hepatic-related comorbidities (e.g., hypertension, diabetes, cardiovascular diseases, Chronic Obstructive Pulmonary Disease [COPD], and malignancies) were also recorded.

5.1. Quality Assessment

Two authors (HM and MV) completed quality assessments independently, using the Joanna Briggs Institute critical appraisal tools (27). These tools assess different items (e.g., selection bias, information bias, and confounding bias) for various study designs, with nine items for case reports, 10 for case series, nine for cross-sectional studies, and 11 for cohort studies.

5.2. Statistical Analysis

Descriptive analyses were used for reporting the results. Continuous variables were summarized as mean and Standard Deviation (SD). Categorical variables were summarized by frequencies and percentages. Differences in continuous and categorical variables were compared using the two-tailed student's t-test and Fisher's exact test, respectively. The P values of less than 0.05 were considered statistically significant. For combining data from studies that reported aggregate-level data with those reporting individual-level data, we weighted the aggregate-level data by the number of patients. The proportion of death among reported patients was also measured and reported. The denominator and nominator for this measure were based on patients with HBV or HCV whose COVID-19 was diagnosed and reported. The main findings were stratified by study design. We also conducted a subgroup analysis by patients' receipt of immunosuppressive therapy.

6. Results

6.1. Study Characteristics

Out of the 941 uniquely identified records, 27 studies met the inclusion criteria and were considered for data extraction. Figure 1 shows the PRISMA flow diagram, and Table 1 presents the study characteristics. Of the 27 included studies with data on SARS-CoV-2 and HBV and/or HCV co-infection, 14 studies reported SARS-CoV-2 and HBV co-infection, 10 studies reported SARS-CoV-2 and HCV co-infection, and three reported both types of co-infection. Regarding their geographical locations, 14 studies reported data from China, three from the USA, two from Spain, two from Brazil, and the remaining six studies from Italy, Austria, Switzerland, the UK, Lithuania, and the UAE (one study each). All of the included studies were observational: 11 studies were case reports, 10 were case series, and six were cross-sectional. The majority of the included studies (n = 21) reported individual-level data, and six studies reported aggregate-level data.

Flowchart of studies included in the systematic review of SARS-CoV-2-HBV or SARS-CoV-2-HCV co-infections
Flowchart of studies included in the systematic review of SARS-CoV-2-HBV or SARS-CoV-2-HCV co-infections
Figure 1.

Flowchart of studies included in the systematic review of SARS-CoV-2-HBV or SARS-CoV-2-HCV co-infections

Table 1.

Characteristics of Studies Included in the Review of SARS-CoV-2-HBV or SARS-CoV-2-HCV Co-infections

First AuthorSettingStudy PeriodaStudy DesignData TypeSample SizeType of HepatitisStudy PopulationQuality Assessment
Aldhaleei (28)UAENot reportedCase reportIndividual1HBVHBV patient7/8
Chen (29)ChinaJan 5 to Feb 20Cross-sectionalAggregate15HBVHBV patients9/9
Chen (30)ChinaJan 20 to Feb 29Cross sectionalAggregate20HBVHBV patients8/9
De Gottardi (31) SwitzerlandMar 4 to Mar 14Case reportIndividual1HCVLT recipient7/8
Fernández (32)SpainMar 5 to Apr 4Case seriesIndividual4 (out of 18)HBV(1), HCV(2) , HBV & HCV(1)OT recipients8/10
Guan (33)ChinaDec 2019 to Jan 20Cross-sectionalAggregate28HBVCOVID-19 patients8/9
Hammami (34)USANot reportedCase reportIndividual1HCVLT recipient7/8
Huang (35)ChinaFeb 1 to Mar 16Case reportIndividual1HBVLT recipient7/8
John Hann (36)UKMar 11 to Apr 12Case seriesIndividual1 (out of 3)HCVLT recipients5/10
Kates (37)USANot reportedCase seriesIndividual1 (out of 4)HCVOT recipients9/10
Kreivenaite (38)LithuaniaMar 16 to Mar 23Case reportIndividual1HCVCirrhosis6/8
Leeb (39)USAMar 18 to Apr 13Case seriesAggregate/Individual2 (out of 28)HCVLT recipients10/10
Li (40)ChinaJan 18 to Feb 26Case seriesIndividual7HBVHBV patients10/10
Liu (41)ChinaJan 23 to Feb 28Case reportIndividual1HBVLT recipient5/8
Liu, J.(42)ChinaJan 1 to Apr 12Cross sectionalAggregate21HBVHBV patients9/9
Loinaz (43)SpainMar 15 to May 5Case seriesIndividual9 (out of 19)HBV(2), HCV(7)LT recipients9/10
Machado (44)BrazilMarch 31 to Apr 12Case reportIndividual1HCVKT recipient7/8
Müller (45)AustriaMar 11 to Apr 9Case reportIndividual1HCV(HIV+)HCV patient6/8
Patrono (46)ItalyMar 5 to Apr 10Case seriesIndividual3HBV(2), HCV(1)LT recipients6/10
Qi (47)ChinaJan 28 to Mar 11Case seriesIndividual1 (out of 3)HBVCirrhosis7/10
Qin(48)ChinaJan 14 to Mar 9Case reportIndividual1HBVLT recipient6/8
Song (49)ChinaDec 2019 to MarCase reportIndividual1 (out of 4)HBVLT recipients5/10
Waisberg (50)BrazilMar to MayCase seriesIndividual1 (out 5)HCVLT recipients9/10
Zhang (51)ChinaJan 24 to Feb 29Cross-sectionalAggregate23HBVHBV patients9/9
Zhao (52)ChinaJan 25 to Jan 30Case reportIndividual1HCV(HIV+)HCV patient6/8
Zhong (53)ChinaJan 14 to Mar 12Case seriesIndividual1 (out of 2)HBVOT recipients6/10
Zou (54)ChinaFeb 1 to Mar 10Cross-sectionalAggregate105HBVHBV patients9/9

Abbreviations: LT: Liver Transplant, OT: Organ Transplant, KT: Kidney Transplant.

aStudy periods refer to 2020 unless otherwise specified.

bLee, B et al. reported COVID-19 in 38 LT recipients, among whom 16 were HCV-positive and two were HBV-positive, but individual information was reported only for the deceased patients that included seven of total LT recipients and two of HCV-positive patients. For 14 HCV-positive and two HBV-positive patients, there was no individual information and we only knew that they were cured at the end of the follow-up.

6.2. Participant Characteristics

As shown in Table 1, study populations were diverse and included organ transplant patients (15 studies), people living with HBV and/or HCV (nine studies), cirrhosis patients (two studies), and a diverse group of COVID-19 patients with hepatic comorbidities (one study). Across the 27 studies, 232 SARS-CoV-2 infections were found in patients with HBV and 22 in patients with HCV. Overall, the mean (SD) age was 49.9 (9.7) and 62.8 (10.3) among patients with HBV and HCV, respectively. Most patients in both groups were male (61.6% among patients with HBV and 72.8% among patients with HCV).

Among SARS-CoV-2-HBV co-infected patients recruited in cross-sectional studies, no one had a history of liver transplantation or immunosuppressive therapy, and only 3.1% (five out of 163) had cirrhosis. These conditions, however, were more commonly reported in case series and case reports of SARS-CoV-2-HBV co-infected patients (Table 2). Among SARS-CoV-2-HCV co-infected patients recruited in case series/reports, 95.2% (20 out of 21) had cirrhosis, and 90.9% (20 of 22) had a history of liver transplantation and immunosuppressive therapy (Table 3).

Table 2.

Demographic and Clinical Characteristics of COVID-19 Patients With Hepatitis B (n = 232) Included in Reviewed Studies Stratified by Study Type

Characteristics (n Reported)Cross-Sectional; No. (%)Case Series/Reports; No. (%)
Age (Mean ± SD)49.6 ± 8.7 [n = 87]50.8 ± 13.5 [n = 20]
Sex n = 212n = 20
Male124 (58.5)19 (95)
Female88 (41.5)1 (5)
Cirrhosisn = 163n = 20
Yes5 (3.1)13 (65)
Liver transplantationn = 163n = 20
Yes0 (0)11 (55)
Immunosuppressive therapyn = 163n = 20
Yes 0 (0)11 (55)
Elevated liver biochemistriesn = 150n = 13
ALT33 (22.0)7 (53.8)
AST39 (26.0)4 (30.8)
ALP3 (2.0)1 (7.7)
GGT20 (13.3)4 (30.7)
Co-morbidities (in addition to COVID-19/hepatitis) n = 164n = 15
At least one morbidity57 (34.7)4 (26.7)
Hypertension33 (20.1)2 (13.3)
Chronic obstructive pulmonary disease3 (1.8)2 (13.3)
Diabetes12 (7.3)4 (26.7)
Cardiovascular disease8 (4.9)0
Malignancy8 (4.9)0
COVID-19 symptoms n = 168n = 20
Fever130 (77.4)17 (86.0)
Cough99 (58.9)13 (65.5)
Dyspnea 66 (39.3)8 (40.0)
Headache 5 (3)0
Arthralgia/myalgia5 (3)3 (15.0)
Fatigue58 (34.5)2 (10.0)
Gastrointestinal symptoms23 (13.7)5 (25.0)
Severityan = 212n = 18
Non-severe128 (60.4)13 (72.2)
Severe84 (39.6)5 (27.8)
Hospitalization n = 211n = 20
Yes211 (100)20 (100)
Intensive care unit admission n = 71n = 18
Yes7 (9.8)5 (27.8)
Death n = 211n = 20
Yes10 (4.7)3 (15)

aMild cases reported as non-severe, and severe/critical cases reported as severe.

Table 3.

Demographic and Clinical Characteristics of COVID-19 Patients With Hepatitis C (n = 22) Included in Reviewed Studies Stratified by Study Type

Characteristics (n Reported)Case Series/Reports; No. (%)
Age (Mean ± SD)62.8 ± 10.3 [n = 22]
Sex n = 22
Male16 (72.8)
Female6 (27.3)
Cirrhosisn = 21
Yes20 (95.2)
Liver transplantationn = 22
Yes20 (90.9)
Immunosuppressive therapyn = 22
Yes 20 (90.9)
Elevated liver biochemistriesn = 13
ALT5 (38.5)
AST5 (38.5)
ALP3 (23.1)
GGT3 of 9 (33.3)
Co-morbidities (in addition to COVID-19/hepatitis) n = 21
At least one morbidity16 (76.2)
Hypertension12 (57.1)
Chronic obstructive pulmonary disease1 (4.8)
Diabetes8 (38.1)
Cardiovascular disease4 (19)
Malignancy0
COVID-19 symptoms n = 22
Fever17 (77.3)
Cough14 (63.6)
Dyspnea 9 (40.9)
Headache 1 (4.5)
Arthralgia/myalgia4 (18.2)
Fatigue4 (18.2)
Gastrointestinal symptoms7 (31.8)
Severityan = 14
Non-Severe11 (78.6)
Severe3 (21.4)
Hospitalization n = 22
Yes19 (86.4)
Intensive care unit admission n = 14
Yes3 (21.4)
Deathbn = 36
Yes3 (8.3)

aMild cases reported as non-severe, and severe/critical cases reported as severe.

bOne of the cross-sectional studies reported COVID-19 in 38 LT recipients, among whom 16 were HCV-positive, and two were HBV-positive, but individual information was reported only for the deceased patients that included seven of total LT recipients and two of HCV-positive patients. For 14 HCV-positive and two HBV-positive patients, there was no individual information, and we only knew that they were cured at the end of the follow-up. Thus, we included them only for the calculation of the proportion of death.

6.3. COVID-19-Related Symptoms

As shown in Table 2 and Table 3, several comorbidities other than COVID-19 and HBV/HCV were reported among the participants. Fever, cough, dyspnea, fatigue, and gastrointestinal symptoms were the most commonly reported COVID-19 symptoms.

Having elevated ALT (i.e., > 45) was found in 22% (33 out of 150) of patients with SARS-CoV-2-HBV co-infection in cross-sectional studies and 53.8% (seven out of 13) in case series/report studies. In addition, elevated AST (i.e., > 45) was found in 26% (39 out of 150) of patients with SARS-CoV-2-HBV co-infection in cross-sectional studies and 30.8% (four out of 13) in case series/report studies. In patients with SARS-CoV-2-HCV co-infection, 38.5% (five out of 13 patients) had elevated ALT and AST.

All of the patients in the SARS-CoV-2-HBV co-infected group and 86.4% (19 of 22) of cases in the SARS-CoV-2-HCV co-infected group were hospitalized. The ICU admission in the SARS-CoV-2-HBV group was reported in 9.8% (seven of 71) of patients in cross-sectional studies and 27.8% (five of 18) in case series/report studies. The ICU admission was reported in 21.4% of patients in the SARS-CoV-2-HCV group. In the SARS-CoV-2-HBV group, severe COVID-19 symptoms were reported in 39.6% (84 of 212) of patients in cross-sectional studies and 27.8% (five of 18) in case series/report studies. In the SARS-CoV-2-HCV group, severe COVID-19 symptoms were reported in 21.4% (three of 14) of patients.

6.4. Death Proportion Among the Patients

Overall, the death proportions were 4.7% (10 out of 211) and 15% (3 out of 20) among SARS-CoV-2-HBV co-infected patients in cross-sectional and case series/report studies, respectively. The three deceased patients in case series/report studies were 73, 53, and 59-years-old and all were males. They had more than one comorbidity, and two of them were on immunosuppressive therapy due to their history of liver transplantation. Information on age and sex of the deceased patients among SARS-CoV-2-HBV co-infected patients in cross-sectional studies was available for two out of 10 patients. Both were male with 38 and 74 years of age.

The proportion of death among SARS-CoV-2-HCV co-infected patients was 8.3 (three of 36). These three patients were 69, 71, and 79-years-old. Two of them were female, and one was male. They had more than one comorbidity and were on immunosuppressive therapy due to their history of liver transplantation.

6.5. COVID-19 Among Immunosuppressed Patients

Based on the available individual data, the mean (SD) age of immunosuppressed patients was significantly higher than that of patients without immunosuppression (60.8 [11.7] vs. 49.9 [12]; P value = 0.001). About 85% (n = 29) of immunosuppressed patients and 66% (n = 31) of patients without immunosuppression were male (P value = 0.04). Overall, 51.5% (n = 17) of patients with immunosuppression and 30% (n = 12) of patients without immunosuppression had at least one comorbidity other than COVID-19 and hepatitis (P value = 0.07). Gastrointestinal symptoms were more common in immunosuppressed patients (32.2% vs. 7.3%; P value = 0.03). The proportions of severe COVID-19 in patients with and without immunosuppression were 33.3% and 30.4%, respectively (P value = 0.7). About 29% of patients with immunosuppression and 12.5% of patients without immunosuppression were admitted to the ICU (P value = 0.1). Death happened in 17.6% of patients with immunosuppression and 6.4% of patients without immunosuppression (P value = 0.1). See Table 4 for further details.

Table 4.

Demographic and Clinical Characteristics of COVID-19 Infection in Hepatitis B and Hepatitis C Patients Included in Reviewed Studies Stratified by Immunosuppression (IS) Status

Characteristics (n reported)IS therapy, No. (%)No IS Therapy, No. (%)P Value
Age (Mean ± SD)(n = 31) 60.8 ± 11.7(n = 22) 49.9 ± 120.001
Sex n = 34n = 47
Male29 (85.3)31 (66)0.04
Female5 (14.7)16 (34)
Co-morbidities (in addition to COVID-19 and hepatitis) n = 33n = 40
At least one morbidity17 (51.5)12 (30)0.09
COVID-19 symptoms n = 34n = 47
Fever27 (79.4)29 (61.7)0.1
Cough20 (58.8)23 (48.9)0.3
Dyspnea 13 (38.2)9 (19.1)0.09
Headache 1 (2.9)2 (4.2)0.3
Arthralgia/myalgia6 (17.6)4 (8.5)0.2
Fatigue6 (17.6)9 (19.1)0.1
Gastrointestinal symptoms11 (32.3)3 (7.3)0.03
Severity n = 24n = 46
Non-severe16 (66.7)32 (69.6)0.7
Severe8 (33.3)14 (30.4)
Hospitalization n = 34n = 470.07
Yes31 (91.2)47 (100)
Intensive care unit admission n = 24n = 320.1
Yes7 (29.2)4 (12.5)
Death n = 34n = 470.1
Yes6 (17.6)3 (6.4)

6.6. Quality Assessment

As shown in Table 1, quality assessment scores of the studies ranged from five to seven for case reports (out of eight possible points), five to 10 for case series (out of 10 possible points), and eight to nine for cross-sectional studies (out of nine possible points). Further details on the quality assessment tools and respective scores are presented in Supplementary File S3.

7. Discussion

We summarized data from 232 patients with SARS-CoV-2 and HBV co-infection and 22 patients with SARS-CoV-2 and HCV co-infection. While our findings are relatively comparable with evidence from COVID-19 patients without hepatic comorbidities (10, 55), our data pointed to higher morbidity and mortality among COVID-19 patients living with HBV, HCV, or both. The most common COVID-19-related symptoms were fever, cough, dyspnea, fatigue, and gastrointestinal symptoms, which have also been common in COVID-19 patients without these co-infections (13, 56-58). Besides, ALT and AST were elevated in a considerable proportion of the patients, a finding that is consistent with estimates of a previous systematic review of earlier studies on liver manifestations among COVID-19 patients, where the pooled incidence of elevated liver chemistries among COVID‐19 patients was reported as 23.1% (13).

Many studies have reported elevated liver biochemistries to be common among COVID‐19 patients with and without hepatic comorbidities (13, 59, 60); however, these findings should be interpreted with caution. First, it is unclear whether the liver damage observed among COVID-19 patients with HBV or HCV comorbidities is mainly due to the adverse impact of SARS-CoV-2 on hepatic cells or patients’ pre-existing viral hepatitis. Second, corticosteroids that are commonly used as a treatment strategy in COVID-19 patients have been shown to increase the risk of hepatitis flare in HBV patients and might impact liver enzyme profiles among these patients (61-63). On the other hand, Chen et al. reported no difference in the level of liver function parameters between COVID-19-infected patients and COVID-19-HBV co-infected patients (30). Kulkarni et al.'s meta-analysis of COVID-19-related liver manifestations reported patients with pre-existing chronic liver disease to have lower odds of developing severe COVID-19 (13). All of these rather equivocal findings highlight the importance of conducting well-controlled studies to fully understand the pathogenesis of SARS-CoV-2 among HBV and HCV patients and determine the main underlying causes of elevated liver enzymes among these multimorbid patients to help improve clinical decision-making.

All of the patients in the SARS-CoV-2-HBV group and 86.4% of cases in the SARS-CoV-2-HCV group were hospitalized. While this finding might be interpreted as a higher risk of hospitalization among these multimorbid populations, it could also be reflective of the fact that the existing evidence is mainly based on, and indeed, skewed towards hospitalized severe COVID-19 patients with hepatic comorbidities. This was further evident in our finding that ICU admission in the SARS-COV-2-HBV co-infected group was estimated at 9.8% and 27.8% in cross-sectional and case series/report studies, respectively. Additionally, ICU admission in the SARS-COV-2-HCV co-infected group was estimated at 21.4%.

The proportions of death were 4.7% and 15% among reported cases of SARS-CoV-2-HBV co-infection in cross-sectional and case series/report studies, and 8.3% of reported cases in SARS-CoV-2-HCV co-infection. Given the high prevalence of multimorbidity among HBV and HCV patients and the fact that most patients included in this review were severe cases of hospitalized COVID-19 with a history of organ transplant, this finding should also be interpreted with caution when compared to the overall risk of mortality among COVID-19 patients, estimated to be around 2% (10). Nonetheless, these findings are informative and suggest that among those who died, multimorbidity, older age, and male sex were common in comparison with all included patients.

Our findings of the impact of immunosuppression on COVID-19 severity are interesting, given the equivocal findings of different studies on the susceptibility of immunosuppressed patients to severe SARS-CoV-2 infection. While some assume a higher risk for the immunosuppressed population due to their systemic immunocompromised status (21, 64), others suggest that immunosuppression may protect against the hyperactivated immune response (65, 66). We found that the proportions of severe COVID-19 cases, admission to the ICU, and death were higher among patients with immunosuppression than in patients without immunosuppression; however, these differences were not statistically significant. These findings are not conclusive and should be interpreted with an eye for the limited sample size in the studies, the observational nature of the studies, the non-matched nature of the comparisons, and the fact that these differences could be due to the immunosuppressed group being more likely to be older, male, and have organ transplants, advanced liver disease, and other extrahepatic comorbidities.

We acknowledge the limitations of our study. First, while most COVID-19 patients are asymptomatic, the available evidence that informed this review included mostly hospitalized patients and was skewed towards more severe patients often with a history of organ transplant and advanced liver disease; therefore, our findings cannot be generalizable to all patients living with COVID-19 and HBV or HCV comorbidities. Second, due to the descriptive design of the included studies and lack of a comparison group, we could not identify factors associated with SARS-CoV-2-HBV or HCV co-infections. Third, most of the included studies were case series and had a small sample size. Therefore, the accurate prevalence of COVID-19 and its manifestations among HBV or HCV subpopulations remains unknown without a population-based survey of patients with these comorbidities. Lastly, given the growing nature of the pandemic and the overwhelmed healthcare systems worldwide, viral hepatitis manifestations may be under-recorded or overlooked during clinical visits, leading to the underestimation of the scope of hepatic manifestations among COVID-19 patients with HBV and HCV comorbidities.

7.1. Conclusions

Despite the limitations of the existing evidence, our data suggest that liver enzyme abnormalities and acute hepatic injuries may be common among COVID-19 patients with HBV and HCV comorbidities. Therefore, these paraclinical profiles should be monitored and examined during clinical visits. While understanding the pathogenesis of SARS-CoV-2 requires further investigations, a careful assessment of hepatic manifestations upon admission could help reduce the multimorbidity among HBV or HCV patients and lead to more favorable health outcomes among COVID-19 patients.

Acknowledgements

References

  • 1.

    Zhu N, Zhang D, Wang W, Li X, Yang B, Song J, et al. A Novel Coronavirus from Patients with Pneumonia in China, 2019. N Engl J Med. 2020;382(8):727-33. [PubMed ID: 31978945]. [PubMed Central ID: PMC7092803]. https://doi.org/10.1056/NEJMoa2001017.

  • 2.

    WHO. WHO Coronavirus Disease (COVID-19) Dashboard. 2020. Available from: https://covid19.who.int/.

  • 3.

    Wang L, He W, Yu X, Hu D, Bao M, Liu H, et al. Coronavirus disease 2019 in elderly patients: Characteristics and prognostic factors based on 4-week follow-up. J Infect. 2020;80(6):639-45. [PubMed ID: 32240670]. [PubMed Central ID: PMC7118526]. https://doi.org/10.1016/j.jinf.2020.03.019.

  • 4.

    Guo W, Li M, Dong Y, Zhou H, Zhang Z, Tian C, et al. Diabetes is a risk factor for the progression and prognosis of COVID-19. Diabetes Metab Res Rev. 2020. e3319. [PubMed ID: 32233013]. [PubMed Central ID: PMC7228407]. https://doi.org/10.1002/dmrr.3319.

  • 5.

    Mostaza JM, Garcia-Iglesias F, Gonzalez-Alegre T, Blanco F, Varas M, Hernandez-Blanco C, et al. Clinical course and prognostic factors of COVID-19 infection in an elderly hospitalized population. Arch Gerontol Geriatr. 2020;91:104204. [PubMed ID: 32771883]. [PubMed Central ID: PMC7383171]. https://doi.org/10.1016/j.archger.2020.104204.

  • 6.

    Foldi M, Farkas N, Kiss S, Zadori N, Vancsa S, Szako L, et al. Obesity is a risk factor for developing critical condition in COVID-19 patients: A systematic review and meta-analysis. Obes Rev. 2020;21(10). e13095. [PubMed ID: 32686331]. [PubMed Central ID: PMC7404429]. https://doi.org/10.1111/obr.13095.

  • 7.

    Fang X, Li S, Yu H, Wang P, Zhang Y, Chen Z, et al. Epidemiological, comorbidity factors with severity and prognosis of COVID-19: a systematic review and meta-analysis. Aging (Albany NY). 2020;12(13):12493-503. [PubMed ID: 32658868]. [PubMed Central ID: PMC7377860]. https://doi.org/10.18632/aging.103579.

  • 8.

    Figliozzi S, Masci PG, Ahmadi N, Tondi L, Koutli E, Aimo A, et al. Predictors of adverse prognosis in COVID-19: A systematic review and meta-analysis. Eur J Clin Invest. 2020;50(10). e13362. [PubMed ID: 32726868]. https://doi.org/10.1111/eci.13362.

  • 9.

    Goyal P, Choi JJ, Pinheiro LC, Schenck EJ, Chen R, Jabri A, et al. Clinical Characteristics of Covid-19 in New York City. N Engl J Med. 2020;382(24):2372-4. [PubMed ID: 32302078]. [PubMed Central ID: PMC7182018]. https://doi.org/10.1056/NEJMc2010419.

  • 10.

    Khalili M, Karamouzian M, Nasiri N, Javadi S, Mirzazadeh A, Sharifi H. Epidemiological characteristics of COVID-19: a systematic review and meta-analysis. Epidemiol Infect. 2020;148. e130. [PubMed ID: 32594937]. [PubMed Central ID: PMC7343974]. https://doi.org/10.1017/S0950268820001430.

  • 11.

    Wander P, Epstein M, Bernstein D. COVID-19 Presenting as Acute Hepatitis. Am J Gastroenterol. 2020;115(6):941-2. [PubMed ID: 32301760]. [PubMed Central ID: PMC7172489]. https://doi.org/10.14309/ajg.0000000000000660.

  • 12.

    Alqahtani SA, Schattenberg JM. Liver injury in COVID-19: The current evidence. United European Gastroenterol J. 2020;8(5):509-19. [PubMed ID: 32450787]. [PubMed Central ID: PMC7268949]. https://doi.org/10.1177/2050640620924157.

  • 13.

    Kulkarni AV, Kumar P, Tevethia HV, Premkumar M, Arab JP, Candia R, et al. Systematic review with meta-analysis: liver manifestations and outcomes in COVID-19. Aliment Pharmacol Ther. 2020;52(4):584-99. [PubMed ID: 32638436]. [PubMed Central ID: PMC7361465]. https://doi.org/10.1111/apt.15916.

  • 14.

    Mehta P, McAuley DF, Brown M, Sanchez E, Tattersall RS, Manson JJ. COVID-19: consider cytokine storm syndromes and immunosuppression. Lancet. 2020;395(10229):1033-4. https://doi.org/10.1016/s0140-6736(20)30628-0.

  • 15.

    Kunutsor SK, Laukkanen JA. Hepatic manifestations and complications of COVID-19: A systematic review and meta-analysis. J Infect. 2020;81(3):e72-4. [PubMed ID: 32579984]. [PubMed Central ID: PMC7306105]. https://doi.org/10.1016/j.jinf.2020.06.043.

  • 16.

    Huang C, Wang Y, Li X, Ren L, Zhao J, Hu Y, et al. Clinical features of patients infected with 2019 novel coronavirus in Wuhan, China. Lancet. 2020;395(10223):497-506. https://doi.org/10.1016/s0140-6736(20)30183-5.

  • 17.

    Guan WJ, Ni ZY, Hu Y, Liang WH, Ou CQ, He JX, et al. Clinical Characteristics of Coronavirus Disease 2019 in China. N Engl J Med. 2020;382(18):1708-20. [PubMed ID: 32109013]. [PubMed Central ID: PMC7092819]. https://doi.org/10.1056/NEJMoa2002032.

  • 18.

    Khinda J, Janjua NZ, Cheng S, van den Heuvel ER, Bhatti P, Darvishian M. Association between markers of immune response at hospital admission and COVID-19 disease severity and mortality: A meta-analysis and meta-regression. J Med Virol. 2021;93(2):1078-98. [PubMed ID: 32776551]. [PubMed Central ID: PMC7436507]. https://doi.org/10.1002/jmv.26411.

  • 19.

    Fan Z, Chen L, Li J, Cheng X, Yang J, Tian C, et al. Clinical Features of COVID-19-Related Liver Functional Abnormality. Clin Gastroenterol Hepatol. 2020;18(7):1561-6. [PubMed ID: 32283325]. [PubMed Central ID: PMC7194865]. https://doi.org/10.1016/j.cgh.2020.04.002.

  • 20.

    Cai Q, Huang D, Yu H, Zhu Z, Xia Z, Su Y, et al. COVID-19: Abnormal liver function tests. J Hepatol. 2020;73(3):566-74. [PubMed ID: 32298767]. [PubMed Central ID: PMC7194951]. https://doi.org/10.1016/j.jhep.2020.04.006.

  • 21.

    Zhang C, Shi L, Wang F. Liver injury in COVID-19: management and challenges. Lancet Gastroenterol Hepatol. 2020;5(5):428-30. https://doi.org/10.1016/s2468-1253(20)30057-1.

  • 22.

    Hernandez Mdel P, Martin P, Simkins J. Infectious Complications After Liver Transplantation. Gastroenterol Hepatol (N Y). 2015;11(11):741-53. [PubMed ID: 27134589]. [PubMed Central ID: PMC4849501].

  • 23.

    Razavi-Shearer D, Gamkrelidze I, Nguyen MH, Chen D, Van Damme P, Abbas Z, et al. Global prevalence, treatment, and prevention of hepatitis B virus infection in 2016: a modelling study. Lancet Gastroenterol Hepatol. 2018;3(6):383-403. https://doi.org/10.1016/s2468-1253(18)30056-6.

  • 24.

    Blach S, Zeuzem S, Manns M, Altraif I, Duberg A, Muljono DH, et al. Global prevalence and genotype distribution of hepatitis C virus infection in 2015: a modelling study. Lancet Gastroenterol Hepatol. 2017;2(3):161-76. https://doi.org/10.1016/s2468-1253(16)30181-9.

  • 25.

    Pressman P, Clemens R, Sahu S, Hayes AW. A review of methanol poisoning: a crisis beyond ocular toxicology. Cutan Ocul Toxicol. 2020;39(3):173-9. [PubMed ID: 32396759]. https://doi.org/10.1080/15569527.2020.1768402.

  • 26.

    Mirzaei H, McFarland W, Karamouzian M, Sharifi H. COVID-19 Among People Living with HIV: A Systematic Review. AIDS Behav. 2020. [PubMed ID: 32734438]. [PubMed Central ID: PMC7391049]. https://doi.org/10.1007/s10461-020-02983-2.

  • 27.

    Joanna Briggs Institute. JBI's Critical Appraisal Tools. 2020. Available from: https://joannabriggs.org/critical-appraisal-tools.

  • 28.

    Aldhaleei WA, Alnuaimi A, Bhagavathula AS. COVID-19 Induced Hepatitis B Virus Reactivation: A Novel Case From the United Arab Emirates. Cureus. 2020;12(6). e8645. [PubMed ID: 32550096]. [PubMed Central ID: PMC7296884]. https://doi.org/10.7759/cureus.8645.

  • 29.

    Chen X, Jiang Q, Ma Z, Ling J, Hu W, Cao Q, et al. Clinical Characteristics of Hospitalized Patients with SARS-CoV-2 and Hepatitis B Virus Co-infection. Virol Sin. 2020. https://doi.org/10.1007/s12250-020-00276-5.

  • 30.

    Chen L, Huang S, Yang J, Cheng X, Shang Z, Lu H, et al. Clinical characteristics in patients with SARS-CoV-2/HBV co-infection. J Viral Hepat. 2020;27(12):1504-7. [PubMed ID: 32668494]. [PubMed Central ID: PMC7404861]. https://doi.org/10.1111/jvh.13362.

  • 31.

    De Gottardi A, Fratila C, Bertoli R, Cerny A, Magenta L, Gianella P, et al. Clinical characteristics and management of a liver transplanted patient admitted with SARS-CoV-2 infection. Clin Res Hepatol Gastroenterol. 2020;44(6):e141-4. [PubMed ID: 32565199]. [PubMed Central ID: PMC7284277]. https://doi.org/10.1016/j.clinre.2020.05.014.

  • 32.

    Fernandez-Ruiz M, Andres A, Loinaz C, Delgado JF, Lopez-Medrano F, San Juan R, et al. COVID-19 in solid organ transplant recipients: A single-center case series from Spain. Am J Transplant. 2020;20(7):1849-58. [PubMed ID: 32301155]. https://doi.org/10.1111/ajt.15929.

  • 33.

    Guan W, Liang W, Zhao Y, Liang H, Chen Z, Li Y, et al. Comorbidity and its impact on 1,590 patients with COVID-19 in China: A Nationwide Analysis. medRxiv. 2020. https://doi.org/10.1101/2020.02.25.20027664.

  • 34.

    Hammami MB, Garibaldi B, Shah P, Liu G, Jain T, Chen PH, et al. Clinical course of COVID-19 in a liver transplant recipient on hemodialysis and response to tocilizumab therapy: A case report. Am J Transplant. 2020;20(8):2254-9. [PubMed ID: 32359210]. [PubMed Central ID: PMC7267667]. https://doi.org/10.1111/ajt.15985.

  • 35.

    Huang JF, Zheng KI, George J, Gao HN, Wei RN, Yan HD, et al. Fatal outcome in a liver transplant recipient with COVID-19. Am J Transplant. 2020;20(7):1907-10. [PubMed ID: 32277591]. [PubMed Central ID: PMC7262021]. https://doi.org/10.1111/ajt.15909.

  • 36.

    John Hann A, Lembach H, McKay SC, Perrin M, Isaac J, Oo YH, et al. Controversies regarding shielding and susceptibility to COVID-19 disease in liver transplant recipients in the United Kingdom. Transpl Infect Dis. 2020;22(5). e13352. [PubMed ID: 32500939]. [PubMed Central ID: PMC7300473]. https://doi.org/10.1111/tid.13352.

  • 37.

    Kates OS, Fisher CE, Stankiewicz-Karita HC, Shepherd AK, Church EC, Kapnadak SG, et al. Earliest cases of coronavirus disease 2019 (COVID-19) identified in solid organ transplant recipients in the United States. Am J Transplant. 2020;20(7):1885-90. [PubMed ID: 32330356]. [PubMed Central ID: PMC7264737]. https://doi.org/10.1111/ajt.15944.

  • 38.

    Kreivenaite E, Gedgaudas R, Valantiene I, Mickiene A, Kupcinskas J. COVID-19 in a Patient with Liver Cirrhosis. J Gastrointestin Liver Dis. 2020;29(2):263-6. [PubMed ID: 32530994]. https://doi.org/10.15403/jgld-2440.

  • 39.

    Lee BT, Perumalswami PV, Im GY, Florman S, Schiano TD, Cobe Study Group. COVID-19 in Liver Transplant Recipients: An Initial Experience From the US Epicenter. Gastroenterology. 2020;159(3):1176-1178 e2. [PubMed ID: 32442561]. [PubMed Central ID: PMC7237372]. https://doi.org/10.1053/j.gastro.2020.05.050.

  • 40.

    Li Y, Li C, Wang J, Zhu C, Zhu L, Ji F, et al. A case series of COVID-19 patients with chronic hepatitis B virus infection. J Med Virol. 2020;92(11):2785-91. [PubMed ID: 32558945]. [PubMed Central ID: PMC7323302]. https://doi.org/10.1002/jmv.26201.

  • 41.

    Liu B, Wang Y, Zhao Y, Shi H, Zeng F, Chen Z. Successful treatment of severe COVID-19 pneumonia in a liver transplant recipient. Am J Transplant. 2020;20(7):1891-5. [PubMed ID: 32243673]. https://doi.org/10.1111/ajt.15901.

  • 42.

    Liu J, Wang T, Cai Q, Sun L, Huang D, Zhou G, et al. Longitudinal changes of liver function and hepatitis B reactivation in COVID-19 patients with pre-existing chronic hepatitis B virus infection. Hepatol Res. 2020;50(11):1211-21. [PubMed ID: 32761993]. [PubMed Central ID: PMC7436737]. https://doi.org/10.1111/hepr.13553.

  • 43.

    Loinaz C, Marcacuzco A, Fernandez-Ruiz M, Caso O, Cambra F, San Juan R, et al. Varied clinical presentation and outcome of SARS-CoV-2 infection in liver transplant recipients: Initial experience at a single center in Madrid, Spain. Transpl Infect Dis. 2020;22(5). e13372. [PubMed ID: 32562561]. [PubMed Central ID: PMC7323090]. https://doi.org/10.1111/tid.13372.

  • 44.

    Machado DJB, Ianhez LE. COVID-19 pneumonia in kidney transplant recipients-Where we are? Transpl Infect Dis. 2020;22(5). e13306. [PubMed ID: 32364677]. [PubMed Central ID: PMC7261981]. https://doi.org/10.1111/tid.13306.

  • 45.

    Muller H, Kniepeiss D, Stauber R, Schrem H, Rauter M, Krause R, et al. Recovery from COVID-19 following hepatitis C, human immunodeficiency virus infection, and liver transplantation. Am J Transplant. 2020;20(11):3255-6. [PubMed ID: 32492753]. [PubMed Central ID: PMC7300671]. https://doi.org/10.1111/ajt.16107.

  • 46.

    Patrono D, Lupo F, Canta F, Mazza E, Mirabella S, Corcione S, et al. Outcome of COVID-19 in liver transplant recipients: A preliminary report from Northwestern Italy. Transpl Infect Dis. 2020;22(5). e13353. [PubMed ID: 32500942]. [PubMed Central ID: PMC7300527]. https://doi.org/10.1111/tid.13353.

  • 47.

    Qi X, Wang J, Li X, Wang Z, Liu Y, Yang H, et al. Clinical course of COVID-19 in patients with pre-existing decompensated cirrhosis: initial report from China. Hepatol Int. 2020;14(4):478-82. [PubMed ID: 32440857]. [PubMed Central ID: PMC7242176]. https://doi.org/10.1007/s12072-020-10051-z.

  • 48.

    Qin J, Wang H, Qin X, Zhang P, Zhu L, Cai J, et al. Perioperative Presentation of COVID-19 Disease in a Liver Transplant Recipient. Hepatology. 2020;72(4):1491-3. [PubMed ID: 32220017]. https://doi.org/10.1002/hep.31257.

  • 49.

    Song SH, Chen TL, Deng LP, Zhang YX, Mo PZ, Gao SC, et al. Clinical characteristics of four cancer patients with SARS-CoV-2 infection in Wuhan, China. Infect Dis Poverty. 2020;9(1):82. [PubMed ID: 32616030]. [PubMed Central ID: PMC7330531]. https://doi.org/10.1186/s40249-020-00707-1.

  • 50.

    Waisberg DR, Abdala E, Nacif LS, Haddad LB, Ducatti L, Santos VR, et al. Liver transplant recipients infected with SARS-CoV-2 in the early postoperative period: Lessons from a single center in the epicenter of the pandemic. Transpl Infect Dis. 2020. e13418. [PubMed ID: 32667716]. [PubMed Central ID: PMC7404440]. https://doi.org/10.1111/tid.13418.

  • 51.

    Zhang B, Huang W, Zhang S. Clinical Features and Outcomes of Coronavirus Disease 2019 (COVID-19) Patients With Chronic Hepatitis B Virus Infection. Clin Gastroenterol Hepatol. 2020;18(11):2633-7. [PubMed ID: 32553905]. https://doi.org/10.1016/j.cgh.2020.06.011.

  • 52.

    Zhao J, Liao X, Wang H, Wei L, Xing M, Liu L, et al. Early Virus Clearance and Delayed Antibody Response in a Case of Coronavirus Disease 2019 (COVID-19) With a History of Coinfection With Human Immunodeficiency Virus Type 1 and Hepatitis C Virus. Clin Infect Dis. 2020;71(16):2233-5. [PubMed ID: 32270178]. [PubMed Central ID: PMC7184426]. https://doi.org/10.1093/cid/ciaa408.

  • 53.

    Zhong Z, Zhang Q, Xia H, Wang A, Liang W, Zhou W, et al. Clinical characteristics and immunosuppressant management of coronavirus disease 2019 in solid organ transplant recipients. Am J Transplant. 2020;20(7):1916-21. [PubMed ID: 32282986]. [PubMed Central ID: PMC7262295]. https://doi.org/10.1111/ajt.15928.

  • 54.

    Zou X, Fang M, Li S, Wu L, Gao B, Gao H, et al. Characteristics of Liver Function in Patients With SARS-CoV-2 and Chronic HBV Coinfection. Clin Gastroenterol Hepatol. 2020. [PubMed ID: 32553907]. [PubMed Central ID: PMC7294291]. https://doi.org/10.1016/j.cgh.2020.06.017.

  • 55.

    Rodriguez-Morales AJ, Cardona-Ospina JA, Gutierrez-Ocampo E, Villamizar-Pena R, Holguin-Rivera Y, Escalera-Antezana JP, et al. Clinical, laboratory and imaging features of COVID-19: A systematic review and meta-analysis. Travel Med Infect Dis. 2020;34:101623. [PubMed ID: 32179124]. [PubMed Central ID: PMC7102608]. https://doi.org/10.1016/j.tmaid.2020.101623.

  • 56.

    Hu Y, Sun J, Dai Z, Deng H, Li X, Huang Q, et al. Prevalence and severity of corona virus disease 2019 (COVID-19): A systematic review and meta-analysis. J Clin Virol. 2020;127:104371. [PubMed ID: 32315817]. [PubMed Central ID: PMC7195434]. https://doi.org/10.1016/j.jcv.2020.104371.

  • 57.

    Chen N, Zhou M, Dong X, Qu J, Gong F, Han Y, et al. Epidemiological and clinical characteristics of 99 cases of 2019 novel coronavirus pneumonia in Wuhan, China: a descriptive study. Lancet. 2020;395(10223):507-13. [PubMed ID: 32007143]. [PubMed Central ID: PMC7135076]. https://doi.org/10.1016/S0140-6736(20)30211-7.

  • 58.

    Zhu J, Ji P, Pang J, Zhong Z, Li H, He C, et al. Clinical characteristics of 3062 COVID-19 patients: A meta-analysis. J Med Virol. 2020;92(10):1902-14. [PubMed ID: 32293716]. [PubMed Central ID: PMC7262119]. https://doi.org/10.1002/jmv.25884.

  • 59.

    Yang X, Yu Y, Xu J, Shu H, Xia J, Liu H, et al. Clinical course and outcomes of critically ill patients with SARS-CoV-2 pneumonia in Wuhan, China: a single-centered, retrospective, observational study. Lancet Respir Med. 2020;8(5):475-81. [PubMed ID: 32105632]. [PubMed Central ID: PMC7102538]. https://doi.org/10.1016/S2213-2600(20)30079-5.

  • 60.

    Xu X, Wu X, Jiang X, Xu K, Ying L, Ma C, et al. Clinical findings in a group of patients infected with the 2019 novel coronavirus (SARS-Cov-2) outside of Wuhan, China: retrospective case series. bmj. 2020;368.

  • 61.

    Shang L, Zhao J, Hu Y, Du R, Cao B. On the use of corticosteroids for 2019-nCoV pneumonia. Lancet. 2020;395(10225):683-4. [PubMed ID: 32122468]. [PubMed Central ID: PMC7159292]. https://doi.org/10.1016/S0140-6736(20)30361-5.

  • 62.

    Wong GL, Wong VW, Yuen BW, Tse YK, Yip TC, Luk HW, et al. Risk of hepatitis B surface antigen seroreversion after corticosteroid treatment in patients with previous hepatitis B virus exposure. J Hepatol. 2020;72(1):57-66. [PubMed ID: 31499132]. https://doi.org/10.1016/j.jhep.2019.08.023.

  • 63.

    Hwang JP, Lok AS. Management of patients with hepatitis B who require immunosuppressive therapy. Nat Rev Gastroenterol Hepatol. 2014;11(4):209-19. [PubMed ID: 24247262]. [PubMed Central ID: PMC4170710]. https://doi.org/10.1038/nrgastro.2013.216.

  • 64.

    Jin X, Lian JS, Hu JH, Gao J, Zheng L, Zhang YM, et al. Epidemiological, clinical and virological characteristics of 74 cases of coronavirus-infected disease 2019 (COVID-19) with gastrointestinal symptoms. Gut. 2020;69(6):1002-9. [PubMed ID: 32213556]. [PubMed Central ID: PMC7133387]. https://doi.org/10.1136/gutjnl-2020-320926.

  • 65.

    D'Antiga L. Coronaviruses and Immunosuppressed Patients: The Facts During the Third Epidemic. Liver Transpl. 2020;26(6):832-4. [PubMed ID: 32196933]. https://doi.org/10.1002/lt.25756.

  • 66.

    Boettler T, Newsome PN, Mondelli MU, Maticic M, Cordero E, Cornberg M, et al. Care of patients with liver disease during the COVID-19 pandemic: EASL-ESCMID position paper. JHEP Rep. 2020;2(3):100113. [PubMed ID: 32289115]. [PubMed Central ID: PMC7128473]. https://doi.org/10.1016/j.jhepr.2020.100113.