The Factors Affecting Bone Density in Cirrhosis

Bone mass at a specific time of life is determined by two factors: peak bone mass accumulated throughout growth, and consolidation and amount of bone loss that will occur thereafter. Peak bone mass, which is mainly determined genetically, can also be affected by environmental factors and lifestyle (1). The amount of bone loss during lifetime is determined by sex, age, body mass index (BMI), nutrition (e.g. the amount of dairy, salt, and protein), alcohol consumption, smoking, physical activity, some medications, and some diseases such as cirrhosis (2, 3). In patients with cirrhosis, pathogenesis of bone mass loss is still unknown (4), and most of our knowledge has been attained from studies on primary biliary cirrhosis (5). In cirrhosis, reduction in bone formation appears to be responsible for decrease in bone density although bone resorption might also have some roles (4). However, it has been shown that there is no significant difference in the T score of the lumbar spine (LS) or femur neck (FN) in patients with cirrhosis in comparison to the same scores in the normal individuals (6). In another study, there was lower bone mineral density (BMD) in patients with cirrhosis compared with that in normal controls (7). In addition, some studies have demonstrated that in patients with cirrhosis, the bone fracture is more prevalent in comparison with the general population, even after adjusting the confounding variables such as sex, presence of cholestasis, and high alcohol consumption (4, 8). The risk of fracture in cirrhosis, which is partly due to decreased BMD, increases by two-fold in comparison with the general population (4). Among existing methods for BMD measurement, dual-energy X-ray absorptiometry (DEXA) is considered as the only practical method, which is clinically feasible (9). Improvement in the treatment of cirrhosis has increased the life expectancy of patients; therefore, special attention should be paid to their quality of life (QOL), which can be influenced by fracture.

In this exploratory study, our aims were to assess bone loss in cirrhosis to determine key factors that can influence it.

This cross-sectional study was conducted during 2008-2010. Totally 97 consecutive patients with cirrhosis were studied who were referred to Razi Hospital, Rasht, Iran. This hospital is an educational and therapeutic referral center for patients with diseases related to all internal medicine fields. The exclusion criteria were as follows: pregnancy, disorders that might lower the accuracy of DEXA, weight > 120 kg, past history of prolonged immobilization, disorders affecting bone metabolism (e.g. thyroid diseases, parathyroid diseases, diabetes, end-stage renal disease, rheumatoid arthritis, ankylosing spondylitis, inherited disorders of connective tissue, sarcoidosis, amyloidosis, hemophilia, thalassemia, hemochromatosis, and malignancies), and drugs affecting bone metabolism (e.g. supplements containing calcium and/or vitamin D, cyclosporin, calcitonin, bisphosphonates, lithium, aluminum hydroxide, levothyroxine, corticosteroids, GnRH agonists, anticoagulants, anticonvulsant, antimetabolites, and cytotoxics). Written informed consent was obtained from all subjects after the approval of the study protocol by the Ethics Committee of Guilan University of Medical Sciences. Cirrhosis was diagnosed using biopsy, paraclinical results (e.g. prolonged prothrombin time, hypoalbuminemia, or increased bilirubin level), and clinical findings including signs and symptoms of cirrhosis, i.e. chronic stigmata of this disease, esophageal varices in endoscopy, or signs of liver cirrhosis in abdominal ultrasonography. Blood samples were also collected in the morning following a 12-hour overnight fast and were tested for calcium (Ca), phosphorus (P), alkaline phosphatase (ALP), blood urea nitrogen (BUN), creatinine (Cr), fasting blood sugar (FBS) (using auto-analyzer/HITACHI 717), and parathyroid hormone (PTH) levels as well as 25-hydroxyvitamin D [25(OH) vitamin D] levels (using ELISA/Stat Fax 3500). Among existing methods for BMD measurement, DEXA is considered as the only practical method, which is clinically feasible (9). Bone densitometry was done in LS (L2-L4) and FN, using DEXA (QDR1000, Hologic, DEXA Inc, Waltham, Massachusetts, the United States). The T score and its definition about osteopenia and osteoporosis were used in our analysis because the mean age of our patients was higher than 50 years (5). In the present study, osteopenia and osteoporosis in an anatomical site were defined as T score ≤ -1 to ≥ -2.5 and T score < -2.5, respectively (3). Diagnosis of osteoporosis was defined as existence of at least one anatomical site (LS or FN) with T score < -2.5. If DEXA-based diagnosis of patients could not be considered as normal bone density or osteoporosis, we categorized the patient as having osteopenia.

The weight of patients was measured after controlling ascites by one calibrated scale and all clinical assessment was performed by a trained assessor. We used Child-Pugh classification for clinical staging of cirrhosis. This reliable staging method has a scoring system of 5 through 15: score ≤ 6, Child-Pugh group A; score 7 through 9, Child-Pugh group B; and score 10 through 15, Child-Pugh group C (10). Glomerular filtration rate (GFR) was calculated by Cockcroft-Gault formula.Statistical analysis was performed using the SPSS 18 (SPSS Inc, Chicago, Illinois, the United States). Normal distribution of continuous variable was determined by Shapiro-Wilks’ test. Correlation test, nonparametric two-related samples t test, Mann-Whitney U test, and logistic regression were also used. P value < 0.05 was considered as statistically significant, but in multiple comparisons (Mann-Whitney U test), the significance was corrected to ≤ 0.01.

Ninety-seven patients with mean age 51 ± 13 years (range, 14 - 82 years) were studied in this research. Their basic characteristics are shown in Table 1. About 80.2% of them had vitamin D deficiency (≤ 30 ng/mL). The median BMI was 22.7 kg/m2 (interquartile range [IQR] = 2.1), and we found that 78.4% (95% CI, 70.2% - 86.6%) of the patients with cirrhosis had abnormal results in DEXA, 45.4% had osteopenia (38.6% at LS, 6.9% at FN, and 54.5% at both sites) and 33% had osteoporosis (75.1% at LS, 9.3 at FN%, and 15.6% at both sites). About 49.5% had calculated GFR (GFRc) ≥ 90 (stage 1), 39.2% had GFRc of 60 through 89 (stage 2), and 11.3% had GFRc of 30 through 59 (stage 3). The most prevalent cause of cirrhosis was hepatitis C (Table 1). There was no significant association between abnormal DEXA findings and the causes of cirrhosis. There was a correlation between T score in LS and FN (Spearman rho = 0.7; P < 0.001). Median of T score in LS was significantly less than that in FN (P < 001). We found differences and similarities in association between variables and T scores of LS and FN (Table 2). Age had significant negative linear correlation only with T score of FN (r = -0.2; P = 0.02), but BMI, Child score, and GFRc had linear correlation with both LS and FN T score (Table 2).

The BMI of the patients with osteoporosis was significantly less than that of the patients with normal BMD (P < 0.001) and osteopenia (P < 0.001). The Child score of patients with osteoporosis was significantly more than that of the patients with normal BMD (P = 0.005) and osteopenia (P < 0.005). Patients with osteoporosis had less GFRc than those with normal BMD did (P = 0.001). Other variables including age, 25 (OH) vitamin D, Ca, P, ALP, PTH, and FBS showed no significant differences between the groups (Table 3). The univariate analysis showed that the risk of abnormal DEXA findings (including both osteoporosis and/or osteopenia in all evaluated sites) did not significantly increased by age, sex, and lower vitamin D levels, but was significantly increased by lower BMI, current smoking, higher CHILD score, and lower GFRc (Table 4). After adjusting variables in a model with six variables including age, sex, BMI, CHILD score, current smoking, GFRc, and vitamin D, we observed that lower vitamin D, high CHILD score, and less GFRc values significantly increased the risk of abnormal DEXA findings (Table 5).

Determinants of BMD such as genetic factors (the main determinant of peak bone mass), sex, age, BMI, lifestyle such as sun exposure, physical activity, smoking, and alcohol consumption, food habits such as salt, protein, and dairy consumption, use of some medications and prevalence of many diseases such as cirrhosis are not similar in different regions of the world. In Iran, 72.1% of men and 75.1% of women have vitamin D deficiency (11), the mean of salt consumption is high (12), 35.7% are physically inactive (13), and 11.8% are current smokers (22.1% of men and 1.3% of women) (14). In our study, 78.4% of patients with cirrhosis had abnormal DEXA findings. In general, among Iranian population, the prevalence rates of osteopenia and osteoporosis in LS are respectively 35% (95% CI, 30% - 39%) and 17% (95% CI, 13% - 20%) (15). Our study showed that the prevalence of both osteopenia (45.4%) and osteoporosis (29.9%) were higher in patients with cirrhosis than in normal population. In patients with cirrhosis the prevalence of osteopenia and osteoporosis has been heterogeneous in different reports, ranging from 11.5% to 48.1% for osteopenia and from 1.9% to 36.6% for osteoporosis (6, 7, 16-19). This heterogeneity can be attributable to the different methodology of the studies, patients’ characteristics definition of osteopenia and osteoporosis (T or Z score), etiologies of cirrhosis, its severity, and factors related to the different parts such as lifestyle and food habits. In Spain, in a descriptive cross-sectional study on 489 patients with cirrhosis (male 79%, mean age of 53 years), Vargas et al. (19) showed that the prevalence of abnormal bone density is 72%. In that study, 59% had alcoholic cirrhosis and 78% had decompensated cirrhosis. In our study, prevalence of alcoholic cirrhosis was much lower, but the rate of decompensated cirrhosis was higher than that of Spanish study. In Turkey, Goral et al. (7) showed that the prevalence of osteoporosis in a study on 55 patients with cirrhosis (males, 69%; mean age, 44.8 years) was 37%. Hepatitis C and primary biliary cirrhosis constituted 2% and 3.6% of etiologies of cirrhosis, respectively. In this study, 41% of the patients had decompensated cirrhosis that was significantly lower than the rate in our study. This may demonstrate the prevalence difference. In another Turkish study on 44 males with cirrhosis (mean age, 50.8 years), Soylu et al. (6) showed that 20% of the patients had osteopenia and 1.9% had osteoporosis, much lower than the rates in our study. In another cross-sectional study in the United Kingdom, Ninkovic et al. (17) showed that the prevalence rates of osteopenia and osteoporosis in patients with cirrhosis were 48.8% and 36.6%, respectively. The characteristics of participants in that study were as follows: mean age, 51.1 years; males, 57.7%; and decompensated cirrhosis, 95%. Etiologies of cirrhosis were alcohol consumption in 18.9%, hepatitis B in 7.4% and primary biliary cirrhosis in 20.6%. Decompensated cirrhosis was reported to be 95% and the etiologies of cirrhosis were significantly different from our study. In a cross-sectional study on 104 patients with cirrhosis who were awaiting for liver transplantation in the United States (males, 51.9%; mean age, 54.4 years), Sokhi et al. (16) demonstrated that 34.6% had osteopenia and 11.5% had osteoporosis. The etiologies of cirrhosis were alcohol consumption (16.3%), hepatitis C (66.4%), cholestatic diseases (7.7%), and other causes (9.6%).

Patients who were awaiting for liver transplantation take more care than the others do. In addition, at the time of this study, all patients were receiving daily multivitamins that included 0.25 μg of vitamin D that might influence the results of bone densitometry. Imbalance in bone resorption and formation varies at different skeletal sites. Bone loss occurs due to both or one of these mechanisms: first, an increase in osteoclastic activity and/or a decrease in osteoblastic activity; and second, increase in the number of remodeling sites (20). Based on our findings, the role of variables in inducing abnormal BMD might be related to the severity of bone loss and its anatomical sites. Researchers in a hybrid of two case-control interview-based study had shown that the role of factors in protection against or inducing osteoporosis in LS could be different from that in FN region. Our study showed that abnormal DEXA was related to severity of cirrhosis but not to the etiology of cirrhosis. Our study showed that the degree of bone loss was related to severity of cirrhosis and not the etiology of chronic liver disease. These are similar to findings of Giouleme et al. (21) study on 83 hospitalized patients with cirrhosis (males, 61.4%; median age of men, 59 years; median age of women, 57 years). The etiologies of cirrhosis were viral hepatitis (49 cases), alcohol (16 cases), primary biliary cirrhosis (1 case), steatohepatitis (1 case), autoimmune hepatitis (2 cases), Wilson disease (1 case), and unknown etiologies (7 cases). Overall, 40.9% of them had decompensated cirrhosis. Some potential inciting factors that either directly or indirectly alter bone mass, i.e. insulin growth factor-1 (IGF-1) deficiency, hyperbilirubinemia, and hypogonadism (estrogen and testosterone deficiency), were commonly seen in cirrhosis by any causes (22). Depression of osteoblastic function may be related to jaundice, independent of etiology (23). Some other inciting factors such as alcoholism, excess tissue iron deposition, and corticosteroid therapy, which could be seen in specific causes of cirrhosis, were infrequently reported in our patients. The effect of etiology on abnormal BMD could be evaluated by conducting a meta-analysis on similar studies, which mentioned different etiologies of chronic liver diseases. In present study, higher Child score and lower vitamin D levels were found to be risk factors for abnormal DEXA findings in patients with cirrhosis. Serum levels of 25 (OH) vitamin D in patients with chronic liver disease were decreased and this level would be reduced more with the progression of cirrhosis (24). It seems that both reduced exposure to UV light and dietary insufficiency account for vitamin D deficiency in the majority of cases. There is also impaired cutaneous synthesis of vitamin D in the presence of jaundice (24). As vitamin D insufficiency is associated with secondary hyperparathyroidism, increased bone turnover, and accelerated bone loss, administration of vitamin D supplementation seems to be a reasonable approach in these patients. Lower GFR was another risk factor for abnormal BMD in our study. Activation of 25 (OH) vitamin D in kidney needs a GFR ≥ 30 mL/min; thus, simultaneous measurement of 25 (OH) vitamin D and 1, 25 (OH) vitamin D must be considered for more precise detection of the role of vitamin D in lowering BMD (24, 25). In patients with cirrhosis, GFR overestimates renal function (20% - 400%); thus, GFR could not estimate real renal function (24, 26-28). This should be mentioned as another important point. In addition, for confirmation of the association between GFR and abnormal DEXA findings in future studies, we suggest direct measurement of renal function by administration of a radioactive isotope (such as inulin, iothalamate), which was not available in our setting.

Our study had some limitations including unknown etiology in some of our cases with cirrhosis, drugs that could not be discontinued, technically overestimation of GFR, and high prevalence of vitamin D insufficiency in our region and the lack of measurement of (1.25)OH Vitamin D.

In conclusion, metabolic bone disease is common among patients with chronic liver disease; osteoporosis and osteopenia account for the majority of cases. Regardless of the etiology of cirrhosis, bone disease in these patients may cause an increased incidence of bone pain and fractures, a major source of morbidity before and after liver transplantation. This study demonstrated that abnormal BMD (osteopenia and/or osteoporosis) is highly prevalent among patients with cirrhosis (78.4%). Higher Child score, vitamin D deficiency, and lower GFR correlate with abnormal BMD of both LS and FN. According to our findings, we recommend correction of vitamin D level in chronic liver disease, especially in patients with cirrhosis to maintain their bone density. We should pay more attention to renal function of this group of patients. These important cares should be emphasized in higher Child score, ie, in patients with decompensated cirrhosis.