Accuracy of Biochemical Markers and Platelet Count for Diagnosis of Liver Fibrosis Staging in Patients with Liver Fibrosis, Loghman Hakim and Taleghani Hospitals, Tehran, Iran (2000-2004)

Wen Zhili; Tan Deming; Liu Gouzhen; Cheng Jun

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Accuracy of Biochemical Markers and Platelet Count for Diagnosis of Liver Fibrosis Staging in Patients with Liver Fibrosis, Loghman Hakim and Taleghani Hospitals, Tehran, Iran (2000-2004)

Author(s):

Wen Zhili^1,*,

Tan Deming²,

Liu Gouzhen²,

Cheng Jun³

1Department of Pathogen Biology, Xiangya Medical College, Central South University & Department of Hepatology, Nanchang 1st Hospital, Nanchang University, wenzhili@126.com, China

2Department of Infectious Diseases, Xiangya Hospital, Central South University, China

3Institute of Infection Diseases, Beijing Ditan Hospital, China

Hepatitis Monthly:Vol. 7, issue 4; 223-228

Published online:Dec 31, 2007

Article type:Research Article

Received:Dec 01, 2007

Accepted:Jan 08, 2008

How to Cite:Zhili W, Deming T, Gouzhen L, Jun C. Accuracy of Biochemical Markers and Platelet Count for Diagnosis of Liver Fibrosis Staging in Patients with Liver Fibrosis, Loghman Hakim and Taleghani Hospitals, Tehran, Iran (2000-2004).Hepat Mon.2007;7(4):223-228.

Abstract

Background and Aims: To initially explore the underlying pathogenesis of the relationship between genotypes of hepatitis B virus (HBV) and its clinical manifestations.

Methods: The S and C genes of HBV from 60 serum samples, infected by HBV of genotypes B or C were amplified by PCR. The products were recombined with vector pEGFP-C1, which is an internal reference for transfection, to construct the eukaryotic expression recombinant plasmids, followed by cloning and subcloning. Then they were transfected into hepatocarcinoma cell HepG2. The increment rates and apoptosis rates of these transfected cells were determinated by MTT and flow cytometer, respectively.

Results: The 120 eukaryotic expression recombinant plasmids were all constructed successfully. As an internal reference for transfection, EGFP confirmed that large S protein and C protein of HBV had been expressed in all HepG2 cells. It was found by flow cytometer that the apoptosis rates of HepG2 cells transfected by pEGFP-C1/HBs or pEGFP-C1/HBc from HBV-genotype C samples were all significantly higher than that from HBV-genotype B samples (P=0.009 & P=0.001, respectively).

Conclusions: HBV of genotype C can induce more serious cell apoptosis than HBV of genotype B. Difference in apoptosis may be an important reason that HBV of genotype C can induce more severe liver injury than HBV of genotype B.

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Author(s):

Wen Zhili:[PubMed][Scholar]
Tan Deming:[PubMed][Scholar]
Liu Gouzhen:[PubMed][Scholar]
Cheng Jun:[PubMed][Scholar]

Official Journal of Research Center for Gastroenterology and Liver Diseases

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Accuracy of Biochemical Markers and Platelet Count for Diagnosis of Liver Fibrosis Staging in Patients with Liver Fibrosis, Loghman Hakim and Taleghani Hospitals, Tehran, Iran (2000-2004)

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