Correlation between HBsAg quantitative assay results and HBV DNA levels in chronic HBV

Azita Ganji; Abbas Esmaeilzadeh; Kamran Ghafarzadegan; Hoda Helalat; Houshang Rafatpanah; Ali Mokhtarifar

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Correlation between HBsAg quantitative assay results and HBV DNA levels in chronic HBV

Author(s):

Azita Ganji^1,*,

Abbas Esmaeilzadeh²,

Kamran Ghafarzadegan²,

Hoda Helalat²,

Houshang Rafatpanah²,

Ali Mokhtarifar²

1Department of Internal Medicine , Imam Reza Hospital, Mashhad University of Medical Sciences, Azita_ganji@yahoo.com, IR Iran

2Department of Internal Medicine , Imam Reza Hospital, Mashhad University of Medical Sciences, IR Iran

Hepatitis Monthly:Vol. 11, issue 5; 342-345

Published online:May 31, 2011

Article type:Research Article

Received:Jun 16, 2010

Accepted:Jan 25, 2011

How to Cite:Ganji A, Esmaeilzadeh A, Ghafarzadegan K, Helalat H, Rafatpanah H, et al. Correlation between HBsAg quantitative assay results and HBV DNA levels in chronic HBV.Hepat Mon.2011;11(5):342-345.

Abstract

Background: Viral load has been used to diagnose and monitor patients who are being treated for chronic hepatitis B (CHB). The Diagnosis methods are molecular-based and expensive. Quantitation of hepatitis B surface antigen (HBsAg) by automated chemiluminescent micro-particle immunoassay has been proposed to be a surrogate marker. Quantitating HBV DNA levels molecularly is expensive; thus, a cheaper laboratory test as a surrogate diagnostic marker might simplify our management.
Objectives:We determined whether quantitative HBsAg levels correlate with HBV DNA levels in CHB.
Patients and Methods: In this cross-sectional study, all CHB patients who were referred by a gastroenterologist to undergo quantitative HBV DNA assay in a qualified laboratory in Mashhad, Iran in 2009 were enrolled, and blood samples was obtained. Patients who were positive for antibodies to HCV and HDV were excluded. HBV DNA was measured by real-time polymerase chain reaction, and serum HBsAg was quantified byelectrochemiluminescence assay (Roche Diagnostic).
Results:Of 97 patients, 70 were male (72%) and 27 were female (28%); the mean age was 39 ± 11 years. Eighty-seven percent wasHBeAg-negative. By Mann-Whitney test,HBSAg titer differed significantly between HBeAg-positive and -negative patients (P = 0.001), as did HBV DNA levels (P = 0.009). By Spearman test, there was no significant correlation between HBsAg and HBV DNA levels (P= 0.606 and r = 0.53).
Conclusions:HBeAg-negative patients have higher levels of HBsAg and lower levels of HBV DNA. By electrochemiluminescence assay,HBsAg has no significant correlation with HBV DNA levels in CHB with predominant genotype D and HBeAg negativity in Iran.

Implication for health policy/practice/research/medical education:
Quantitative detection of HBsAg as one of the new diagnostic methods is discussed recently in many papers. This article opens new windows toward basic scientists, clinicians in the field of hepatology and infection diseases.
Please cite this paper as:
Ganji A, Esmaeilzadeh A, Ghafarzadegan K, Helalat H, Rafatpanah H, Mokhtarifar A. Correlation between HBsAg quantitative assay results and HBV DNA levels in chronic HBV. Hepat Mon.2011;11(5):342-345.

Keywords

Chronic hepatitis B

Quantitative HBsAg

HBV DNA level

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Author(s):

Azita Ganji:[PubMed][Scholar]
Abbas Esmaeilzadeh:[PubMed][Scholar]
Kamran Ghafarzadegan:[PubMed][Scholar]
Hoda Helalat:[PubMed][Scholar]
Houshang Rafatpanah:[PubMed][Scholar]
Ali Mokhtarifar:[PubMed][Scholar]

Official Journal of Research Center for Gastroenterology and Liver Diseases

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Correlation between HBsAg quantitative assay results and HBV DNA levels in chronic HBV

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