Polymorphisms within the Promoter Region of the Gamma Interferon (IFN-?) Receptor1 Gene are Associated with the Susceptibility to Chronic HBV Infection in an Iranian Population

authors:

avatar Sayyad Khanizadeh ORCID 1 , avatar Mehrdad Ravanshad ORCID 1 , * , avatar Seyed Reza Reza Mohebbi ORCID 2 , * , avatar Hamed Naghoosi ORCID 2 , avatar Mohamad Ebrahim Abrahim Tahaei ORCID 2 , avatar Seyed Dawood Dawood Mousavi Nasab ORCID 1 , avatar Sara Romani ORCID 2 , avatar Pedram Azimzadeh ORCID 2 , avatar Azar Sanati ORCID 2 , avatar Mohammad Reza Reza Zali ORCID 2

Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, ravanshad@modares.ac.ir, IR Iran
Research Center for Gastroenterology and Liver Disease, Shaheed Beheshti University of Medical Sciences, srmohebbi@gmail.com, IR Iran
Corresponding Authors:
Hepatitis Monthly: Vol.12, issue 11; 7283
published online: November 6, 2012
article type: Research Article
received: July 17, 2012
accepted: September 15, 2012
DOI: https://doi.org/10.5812/hepatmon.7283

how to cite: Khanizadeh S, Ravanshad M , Mohebbi S R, Naghoosi H , Tahaei M E, et al. Polymorphisms within the Promoter Region of the Gamma Interferon (IFN-?) Receptor1 Gene are Associated with the Susceptibility to Chronic HBV Infection in an Iranian Population. Hepat Mon. 2012;12(11):7283. https://doi.org/10.5812/hepatmon.7283.

Abstract

Background:

chronic hepatitis B virus (HBV) infection is a multifactorial disease that can result in serious clinical complications. Host genetic background especially the genes that encode immunologic factors like INF-? and its receptor (IFN-? R) are critical in the pathogenesis of infection.

Objectives:

The current study aimed to investigate the association between two single nucleotide polymorphisms (SNPs) at positions -611 and -56 within the promoter region of gamma interferon receptor1 gene (IFN-? R1) and chronic HBV infection.

Materials and Methods:

Genomic DNA from peripheral blood samples of 200 chronically HBV infected patients and 200 healthy blood donors, as controls, were collected and genomic DNA was extracted by phenol-chloroform method and DNA analysis genotype identification was performed by PCR-RFLP.

Results:

The results indicated that both SNPs frequency had a significant difference in the patient and control groups. At position -56, TT genotype was associated with patient group and P value was 0.002 and at position -611, GG genotype was further observed in control group and P value was 0.006.

Conclusions:

Presence of G allele at position -611 within promoter of IFN-? R1 gene in the enrolled population for the study was related to lower risk of disease, and presence of T allele at position -56 was also related to susceptibility to chronic HBV infection. Men had higher frequency of chronic HBV infection, which might be the result of high risk behavior.

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