https://doi.org/10.5812/hepatmon.7283

Polymorphisms within the Promoter Region of the Gamma Interferon (IFN-?) Receptor1 Gene are Associated with the Susceptibility to Chronic HBV Infection in an Iranian Population

authors:

avatar Sayyad Khanizadeh ORCID 1 , avatar Mehrdad Ravanshad ORCID 1 , * , avatar Seyed Reza Reza Mohebbi ORCID 2 , * , avatar Hamed Naghoosi ORCID 2 , avatar Mohamad Ebrahim Abrahim Tahaei ORCID 2 , avatar Seyed Dawood Dawood Mousavi Nasab ORCID 1 , avatar Sara Romani ORCID 2 , avatar Pedram Azimzadeh ORCID 2 , avatar Azar Sanati ORCID 2 , avatar Mohammad Reza Reza Zali ORCID 2

Department of Virology, Faculty of Medical Sciences, Tarbiat Modares University, ravanshad@modares.ac.ir, IR Iran
Research Center for Gastroenterology and Liver Disease, Shaheed Beheshti University of Medical Sciences, srmohebbi@gmail.com, IR Iran
Corresponding Authors:
Hepatitis Monthly: Vol.12, issue 11; 7283
published online: November 6, 2012
article type: Research Article
received: July 17, 2012
accepted: September 15, 2012

How To Cite Khanizadeh S, Ravanshad M , Mohebbi S R, Naghoosi H , Tahaei M E, et al. Polymorphisms within the Promoter Region of the Gamma Interferon (IFN-?) Receptor1 Gene are Associated with the Susceptibility to Chronic HBV Infection in an Iranian Population. Hepat Mon. 2012;12(11):7283. https://doi.org/10.5812/hepatmon.7283.

Abstract

Background:

chronic hepatitis B virus (HBV) infection is a multifactorial disease that can result in serious clinical complications. Host genetic background especially the genes that encode immunologic factors like INF-? and its receptor (IFN-? R) are critical in the pathogenesis of infection.

Objectives:

The current study aimed to investigate the association between two single nucleotide polymorphisms (SNPs) at positions -611 and -56 within the promoter region of gamma interferon receptor1 gene (IFN-? R1) and chronic HBV infection.

Materials and Methods:

Genomic DNA from peripheral blood samples of 200 chronically HBV infected patients and 200 healthy blood donors, as controls, were collected and genomic DNA was extracted by phenol-chloroform method and DNA analysis genotype identification was performed by PCR-RFLP.

Results:

The results indicated that both SNPs frequency had a significant difference in the patient and control groups. At position -56, TT genotype was associated with patient group and P value was 0.002 and at position -611, GG genotype was further observed in control group and P value was 0.006.

Conclusions:

Presence of G allele at position -611 within promoter of IFN-? R1 gene in the enrolled population for the study was related to lower risk of disease, and presence of T allele at position -56 was also related to susceptibility to chronic HBV infection. Men had higher frequency of chronic HBV infection, which might be the result of high risk behavior.

Full Text

Full text is available in PDF