Drug-Related Mutational Patterns in Hepatitis B Virus (HBV) Reverse Transcriptase Proteins From Iranian Treatment-Naïve Chronic HBV Patients

Mostafa Mahabadi; Mehdi Norouzi; Seyed Moayed Alavian; katayoun samimirad; Talat Mokhtari Azad; Esmaeil Saberfar; Mahmood Mahmoodi; fatemeh ramezani; Hadi Karimzadeh; Reza Malekzadeh; G montazeri; Azim Nejatizadeh; Masood Ziaee; Farshid Abedi; behrooz ataei; Majid Yaran; Babak Sayad; Mohammad Somi; Gholamreza Sarizadeh; Ismaeil Sanei-Moghaddam; Fariborz Mansour-Ghanaei; Houshang Rafatpanah; Mohammad Amin Pourhosseingholi; Hossein Keyvani; Ebrahim Kalantari; Mehdi Saberifiroozi; Mohammad Ali Judaki; Shiva Ghamari; Maryam Daram; Zeinab Fazeli; Zahra Goodarzi; Abulfazl Khedive; Abdolvahab Moradi; Seyed Mohammad Jazayeri

doi:10.5812/hepatmon.6712

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https://doi.org/ 10.5812/hepatmon.6712

Drug-Related Mutational Patterns in Hepatitis B Virus (HBV) Reverse Transcriptase Proteins From Iranian Treatment-Naïve Chronic HBV Patients

Authors:

Mostafa Mahabadi¹,

Mehdi Norouzi¹,

Seyed Moayed Alavian²,

katayoun samimirad³,

Talat Mokhtari Azad¹,

Esmaeil Saberfar⁴,

Mahmood Mahmoodi⁵,

fatemeh ramezani¹,

Hadi Karimzadeh¹,

Reza Malekzadeh⁶,

G montazeri⁶,

Azim Nejatizadeh⁷,

Masood Ziaee⁸,

Farshid Abedi⁹,

behrooz ataei¹⁰,

Majid Yaran¹⁰,

Babak Sayad¹¹,

Mohammad Somi¹²,

Gholamreza Sarizadeh¹³,

Ismaeil Sanei-Moghaddam¹⁴,

Fariborz Mansour-Ghanaei¹⁵,

Houshang Rafatpanah¹⁶,

Mohammad Amin Pourhosseingholi¹⁷,

Hossein Keyvani¹⁸,

Ebrahim Kalantari¹⁹,

Mehdi Saberifiroozi²⁰,

Mohammad Ali Judaki¹,

Shiva Ghamari¹,

Maryam Daram¹,

Zeinab Fazeli¹,

Zahra Goodarzi⁴,

Abulfazl Khedive¹,

Abdolvahab Moradi²¹,

Seyed Mohammad Jazayeri^1,*

1Hepatitis B Molecular Laboratory, Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran

2Middle East Liver Diseases Center (MELD Centers), Tehran, IR Iran

3Hepatitis C Molecular Laboratory, Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran

4The research and development department of Bayerpaul vaccine and pharmaceutical company, Tehran, IR Iran

5Department of Epidemiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran

6Digestive Disease Research Center, Tehran University of Medical Sciences, Tehran, IR Iran

7Research Center for Molecular Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, IR Iran

8Hepatitis Research Center , Department of Internal Medicine, Faculty of medicine, Birjand University of Medical Sciences, Birjand, IR Iran

9Department of Infectious Disease, Mashhad University of Medical Sciences, Mashhad, IR Iran

10Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, IR Iran

11Kermanshah Liver Diseases and Hepatitis Research Center, Kermanshah, IR Iran

12Liver and Gastrointestinal Disease Research Center, Tabriz University of Medical Sciences, Tabriz, IR Iran

13Educational Region of Khouzestan Blood Transfusion Organization, Ahvaz, IR Iran

14Department of Gastroenterology, Zahedan University of Medical Sciences, Zahedan, IR Iran

15Gastrointestinal and Liver Diseases Research Center, Guilan University of Medical Sciences, Rasht, IR Iran

16Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, IR Iran

17Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran

18Department of Virology, School of Medicine, Tehran University of Medical Sciences, Tehran, IR Iran

19Gholhack Medical Laboratory, Tehran, IR Iran

20Gastroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz, IR Iran

21Department of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, IR Iran

Hepatitis Monthly:Vol. 13, issue 1; e93042

Published online:Jan 20, 2013

Article type:Research Article

Received:May 06, 2019

Accepted:Nov 12, 2012

How to Cite:Mostafa MahabadiMehdi NorouziSeyed Moayed Alaviankatayoun samimiradTalat Mokhtari AzadEsmaeil SaberfarMahmood Mahmoodifatemeh ramezaniHadi KarimzadehReza MalekzadehG montazeriAzim NejatizadehMasood ZiaeeFarshid Abedibehrooz ataeiMajid YaranBabak SayadMohammad SomiGholamreza SarizadehIsmaeil Sanei-MoghaddamFariborz Mansour-GhanaeiHoushang RafatpanahMohammad Amin PourhosseingholiHossein KeyvaniEbrahim KalantariMehdi SaberifirooziMohammad Ali JudakiShiva GhamariMaryam DaramZeinab FazeliZahra GoodarziAbulfazl KhediveAbdolvahab MoradiSeyed Mohammad Jazayeriet al.Drug-Related Mutational Patterns in Hepatitis B Virus (HBV) Reverse Transcriptase Proteins From Iranian Treatment-Naïve Chronic HBV Patients.13(1):e93042.https://doi.org/ 10.5812/hepatmon.6712.

Abstract

Background: Immunomodulators and Nucleotide analogues have been used globally for the dealing of chronic hepatitis B virus (HBV) infection. However, the development of drug resistance is a major limitation to their long-term effectiveness.

Objectives: The aim of this study was to characterize the hepatitis B virus reverse transcriptase (RT) protein variations among Iranian chronic HBV carriers who did not receive any antiviral treatments.

Materials and Methods: Hepatitis B virus partial RT genes from 325 chronic in active carrier patients were amplified and directly sequenced. Nucleotide/amino acid substitutions were identified compared to the sequences obtained from the database.

Results: All strains belonging to genotype D.365 amino-acid substitutions were found. Mutations related to lamivudine, adefovir, telbivudine, and entecavir occurred in (YMDD) 4% (n = 13), (SVQ) 17.23% (n = 56), (M204I/V + L180M) 2.45% (n = 8) and (M204I) 2.76% (n = 9) of patients, respectively.

Conclusions: RT mutants do occur naturally and could be found in HBV carriers who have never received antiviral therapy. However, mutations related to drug resistance in Iranian treatment-naïve chronic HBV patients were found to be higher than other studies published formerly. Chronic HBV patients should be monitored closely prior the commencement of therapy to achieve the best regimen option.

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Search Relations

Author(s):

Mostafa Mahabadi:[PubMed][Scholar]
Mehdi Norouzi:[PubMed][Scholar]
Seyed Moayed Alavian:[PubMed][Scholar]
katayoun samimirad:[PubMed][Scholar]
Talat Mokhtari Azad:[PubMed][Scholar]
Esmaeil Saberfar:[PubMed][Scholar]
Mahmood Mahmoodi:[PubMed][Scholar]
fatemeh ramezani:[PubMed][Scholar]
Hadi Karimzadeh:[PubMed][Scholar]
Reza Malekzadeh:[PubMed][Scholar]
G montazeri:[PubMed][Scholar]
Azim Nejatizadeh:[PubMed][Scholar]
Masood Ziaee:[PubMed][Scholar]
Farshid Abedi:[PubMed][Scholar]
behrooz ataei:[PubMed][Scholar]
Majid Yaran:[PubMed][Scholar]
Babak Sayad:[PubMed][Scholar]
Mohammad Somi:[PubMed][Scholar]
Gholamreza Sarizadeh:[PubMed][Scholar]
Ismaeil Sanei-Moghaddam:[PubMed][Scholar]
Fariborz Mansour-Ghanaei:[PubMed][Scholar]
Houshang Rafatpanah:[PubMed][Scholar]
Mohammad Amin Pourhosseingholi:[PubMed][Scholar]
Hossein Keyvani:[PubMed][Scholar]
Ebrahim Kalantari:[PubMed][Scholar]
Mehdi Saberifiroozi:[PubMed][Scholar]
Mohammad Ali Judaki:[PubMed][Scholar]
Shiva Ghamari:[PubMed][Scholar]
Maryam Daram:[PubMed][Scholar]
Zeinab Fazeli:[PubMed][Scholar]
Zahra Goodarzi:[PubMed][Scholar]
Abulfazl Khedive:[PubMed][Scholar]
Abdolvahab Moradi:[PubMed][Scholar]
Seyed Mohammad Jazayeri:[PubMed][Scholar]