Drug-Related Mutational Patterns in Hepatitis B Virus (HBV) Reverse Transcriptase Proteins From Iranian Treatment-Naïve Chronic HBV Patients

authors:

avatar Mostafa Mahabadi 1 , avatar Mehdi Norouzi 1 , avatar Seyed Moayed Alavian 2 , avatar katayoun samimirad 3 , avatar Talat Mokhtari Azad 1 , avatar Esmaeil Saberfar 4 , avatar Mahmood Mahmoodi 5 , avatar fatemeh ramezani 1 , avatar Hadi Karimzadeh 1 , avatar Reza Malekzadeh 6 , avatar G montazeri 6 , avatar Azim Nejatizadeh 7 , avatar Masood Ziaee 8 , avatar Farshid Abedi 9 , avatar behrooz ataei 10 , avatar Majid Yaran 10 , avatar Babak Sayad 11 , avatar Mohammad Somi 12 , avatar Gholamreza Sarizadeh 13 , avatar Ismaeil Sanei-Moghaddam 14 , avatar Fariborz Mansour-Ghanaei 15 , avatar Houshang Rafatpanah 16 , avatar Mohammad Amin Pourhosseingholi 17 , avatar Hossein Keyvani 18 , avatar Ebrahim Kalantari 19 , avatar Mehdi Saberifiroozi 20 , avatar Mohammad Ali Judaki 1 , avatar Shiva Ghamari 1 , avatar Maryam Daram 1 , avatar Zeinab Fazeli 1 , avatar Zahra Goodarzi 4 , avatar Abulfazl Khedive 1 , avatar Abdolvahab Moradi 21 , avatar Seyed Mohammad Jazayeri 1 , *

Hepatitis B Molecular Laboratory, Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran
Middle East Liver Diseases Center (MELD Centers), Tehran, IR Iran
Hepatitis C Molecular Laboratory, Department of Virology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran
The research and development department of Bayerpaul vaccine and pharmaceutical company, Tehran, IR Iran
Department of Epidemiology, School of Public Health, Tehran University of Medical Sciences, Tehran, IR Iran
Digestive Disease Research Center, Tehran University of Medical Sciences, Tehran, IR Iran
Research Center for Molecular Medicine, Hormozgan University of Medical Sciences, Bandar Abbas, IR Iran
Hepatitis Research Center , Department of Internal Medicine, Faculty of medicine, Birjand University of Medical Sciences, Birjand, IR Iran
Department of Infectious Disease, Mashhad University of Medical Sciences, Mashhad, IR Iran
Infectious Diseases and Tropical Medicine Research Center, Isfahan University of Medical Sciences, Isfahan, IR Iran
Kermanshah Liver Diseases and Hepatitis Research Center, Kermanshah, IR Iran
Liver and Gastrointestinal Disease Research Center, Tabriz University of Medical Sciences, Tabriz, IR Iran
Educational Region of Khouzestan Blood Transfusion Organization, Ahvaz, IR Iran
Department of Gastroenterology, Zahedan University of Medical Sciences, Zahedan, IR Iran
Gastrointestinal and Liver Diseases Research Center, Guilan University of Medical Sciences, Rasht, IR Iran
Immunology Research Center, Mashhad University of Medical Sciences, Mashhad, IR Iran
Gastroenterology and Liver Diseases Research Center, Shahid Beheshti University of Medical Sciences, Tehran, IR Iran
Department of Virology, School of Medicine, Tehran University of Medical Sciences, Tehran, IR Iran
Gholhack Medical Laboratory, Tehran, IR Iran
Gastroenterohepatology Research Center, Shiraz University of Medical Sciences, Shiraz, IR Iran
Department of Microbiology, School of Medicine, Golestan University of Medical Sciences, Gorgan, IR Iran

how to cite: Mahabadi M, Norouzi M, Alavian S M, samimirad K, Mokhtari Azad T, et al. Drug-Related Mutational Patterns in Hepatitis B Virus (HBV) Reverse Transcriptase Proteins From Iranian Treatment-Naïve Chronic HBV Patients. Hepat Mon. 2013;13(1):e93042. doi: 10.5812/hepatmon.6712.

Abstract

Background: Immunomodulators and Nucleotide analogues have been used globally for the dealing of chronic hepatitis B virus (HBV) infection. However, the development of drug resistance is a major limitation to their long-term effectiveness.
Objectives: The aim of this study was to characterize the hepatitis B virus reverse transcriptase (RT) protein variations among Iranian chronic HBV carriers who did not receive any antiviral treatments.
Materials and Methods: Hepatitis B virus partial RT genes from 325 chronic in active carrier patients were amplified and directly sequenced. Nucleotide/amino acid substitutions were identified compared to the sequences obtained from the database.
Results: All strains belonging to genotype D.365 amino-acid substitutions were found. Mutations related to lamivudine, adefovir, telbivudine, and entecavir occurred in (YMDD) 4% (n = 13), (SVQ) 17.23% (n = 56), (M204I/V + L180M) 2.45% (n = 8) and (M204I) 2.76% (n = 9) of patients, respectively.
Conclusions: RT mutants do occur naturally and could be found in HBV carriers who have never received antiviral therapy. However, mutations related to drug resistance in Iranian treatment-naïve chronic HBV patients were found to be higher than other studies published formerly. Chronic HBV patients should be monitored closely prior the commencement of therapy to achieve the best regimen option.

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