Adipocyte Derived Hormones Gene Expression, Resistin and Visfatin, in AGS Gastric Cancer Cell Line

authors:

avatar Masumeh Gorgian Mohammadi 1 , avatar Mehdi Hedayati 2 , * , avatar Nosratollah Zarghami 3 , avatar Sara Ghaemmaghami 1 , avatar Mojtaba Mohaddes 4

Dept. of Clinical Biochemistry, Tabriz University of Medical Sciences, Tabriz, Dept. of Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences,Tehran, Iran
Dept. of Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences,Tehran, Iran
Dept. of Clinical Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Dept. of Medical Genetic, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran

how to cite: Gorgian Mohammadi M, Hedayati M , Zarghami N, Ghaemmaghami S, Mohaddes M . Adipocyte Derived Hormones Gene Expression, Resistin and Visfatin, in AGS Gastric Cancer Cell Line. Int J Cancer Manag. 2013;6(3):e80431.

Abstract

Background: Adipose tissue has characteristics of an endocrine organ which releases a number of adipocyte-specific factors, known as adipocytokines. It has recently suggested that adipocytokines might play a role in pathogenesis and progression of certain cancers, especially in gastric cancer. This study has managed to investigate endogenous and/or exogenous expression of Visfatin and Resistin in gastric cancer cell line.
Methods: Cell culture and semi-quantitative reverse transcription polymerase chain reaction has performed to measure mRNA and protein expression of Resistin and Visfatin in gastric cancer cell lines. ELISA test has performed for cell lysate and supernatant of cell culture to measure Resistin and Visfatin protein expression and secretion.
Results: Human gastric cancer cell line (AGS cell line) has found to express Visfatin mRNA and protein but Resistin mRNA and protein has not expressed.
Conclusion: Visfatin has expressed endogenously in AGS human gastric cancer cells. Conversely Resistin has no expression. The results of this study has suggested that expression of adipocytokine proteins in real samples, could be a biomarker for gastric cancer.

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