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International Journal of Cancer Management
The Official Journal of Cancer Research Center (CRC), Shahid Beheshti University of Medical Sciences
e-ISSN 2538-497X
p-ISSN 2538-4422
Adipocyte Derived Hormones Gene Expression, Resistin and Visfatin, in AGS Gastric Cancer Cell Line
Authors
Masumeh Gorgian Mohammadi1, Mehdi Hedayati2,*, Nosratollah Zarghami3, Sara Ghaemmaghami1, Mojtaba Mohaddes4
1Dept. of Clinical Biochemistry, Tabriz University of Medical Sciences, Tabriz, Dept. of Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences,Tehran, Iran
2Dept. of Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences,Tehran, Iran
3Dept. of Clinical Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
4Dept. of Medical Genetic, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
*Corresponding Author: Corresponding author: Mehdi Hedayati, Dept. of Cellular and Molecular Endocrine Research Center, Research Institute for Endocrine Sciences, Shahid Beheshti University of Medical Sciences,Tehran, Iran, Tel: +98-2122432500, E-mail: Email: [email protected]
Published online:Sep 30, 2013
Article type:Research Article
Received:Jul 16, 2013
Accepted:Aug 17, 2013
How to Cite:Gorgian Mohammadi M, Hedayati M, Zarghami N, Ghaemmaghami S, Mohaddes M. Adipocyte Derived Hormones Gene Expression, Resistin and Visfatin, in AGS Gastric Cancer Cell Line. Int J Cancer Manag. 2013;6(3):e80431. doi:
Abstract
Background: Adipose tissue has characteristics of an endocrine organ which releases a number of adipocyte-specific factors, known as adipocytokines. It has recently suggested that adipocytokines might play a role in pathogenesis and progression of certain cancers, especially in gastric cancer. This study has managed to investigate endogenous and/or exogenous expression of Visfatin and Resistin in gastric cancer cell line.
Methods: Cell culture and semi-quantitative reverse transcription polymerase chain reaction has performed to measure mRNA and protein expression of Resistin and Visfatin in gastric cancer cell lines. ELISA test has performed for cell lysate and supernatant of cell culture to measure Resistin and Visfatin protein expression and secretion.
Results: Human gastric cancer cell line (AGS cell line) has found to express Visfatin mRNA and protein but Resistin mRNA and protein has not expressed.
Conclusion: Visfatin has expressed endogenously in AGS human gastric cancer cells. Conversely Resistin has no expression. The results of this study has suggested that expression of adipocytokine proteins in real samples, could be a biomarker for gastric cancer.
Copyright
© 2013, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
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