Logo

Effect of Ghrelin on Aldolase Gene Expression in the Heart of Chronic Hypoxic Rat

Author(s):
Mohammad Reza AliparastiMohammad Reza Aliparasti2, Mohammad Reza AlipourMohammad Reza Alipour3, Shohreh AlmasiShohreh Almasi2, Hadi FeiziHadi Feizi4,*
2Department of Immunology, Tabriz University of Medical Sciences, IR Iran
3Tuberculosis and Lung Research Center, Tabriz University of Medical Sciences, IR Iran
4Department of Physiology, Hormozgan University of Medical Sciences, [email protected], IR Iran

International Journal of Endocrinology and Metabolism:Vol. 10, issue 3; 553-557
Published online:Jun 30, 2012
Article type:Research Article
Received:Dec 19, 2011
Accepted:Mar 13, 2012
How to Cite:Mohammad Reza Aliparasti, Mohammad Reza Alipour, Shohreh Almasi, Hadi Feizi, Effect of Ghrelin on Aldolase Gene Expression in the Heart of Chronic Hypoxic Rat.Int J Endocrinol Metab.2012;10(3):553-557.https://doi.org/10.5812/ijem.3914.

Abstract

Background:

Chronic hypoxia causes apoptosis of cardiac myocytes, however, energy production by anaerobic glycolysis protects myocardium against hypoxia injuries. Aldolase A is a well-characterised key enzyme of the glycolysis pathway. Ghrelin, a 28-amino-acid peptide, synthesizes in the stomach and has protective roles in cardiovascular systems and also affects metabolic pathways.

Objectives:

Therefore, the aim of this study was to evaluate the effect of ghrelin on aldolase A gene expression after chronic hypoxia in the rat hearts.

Materials and Methods:

Twenty four adult male wistar rats were randomly divided into three groups. Hypoxic rats with saline or ghrelin treatment were placed in a normobaric hypoxic chamber (O2 11 %), for two weeks. Controls remained in room air. Aldolase A gene expression was measured by Real-Time RT-PCR.

Results:

the transcriptiom rate of Aldolase A in hypoxic animals did not change significantly compared to negative control ones. During chronic hypoxia, ghrelin treatment increased the amount of heart Aldolase A gene expression compared to negative controls (P = 0.029). Hypoxic animals that were treated with ghrelin were significantly more polycythemic than the controls and even hypoxic with saline treated rats (P < 0.001).

Conclusions:

It seems that ghrelin interferes in the cardiac metabolism through up-regulation of glycolytic enzymes. In other words, it may protect heart from possible hypoxia induced damages.

Full Text

Full text is available in PDF

comments

Leave a comment here


Crossmark
Crossmark
Checking
Share on
Metrics

Purchasing Reprints

  • Copyright Clearance Center (CCC) handles bulk orders for article reprints for Brieflands. To place an order for reprints, please click here (   https://www.copyright.com/landing/reprintsinquiryform/ ). Clicking this link will bring you to a CCC request form where you can provide the details of your order. Once complete, please click the ‘Submit Request’ button and CCC’s Reprints Services team will generate a quote for your review.
Search Relations

Author(s):

Related Articles