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Background:
Ghrelin plays an important role in the regulation of food intake and body weight. It also decreases testosterone and opioid secretion.
International Journal of Endocrinology and Metabolism
Research Institute for Endocrine Sciences
Effect of Interaction Between Testosterone and Morphine on Serum Ghrelin Concentration in Sheep Fed on Different Dietary Energy Levels
Authors:
1Faculty of Biology Science, Shahid Beheshti University, azn_araz@yahoo.com, IR Iran
2Faculty of Physiology, Shahid Beheshti University, IR Iran
3Applied Scientific Education Institute of Jahad Keshavarzi, IR Iran
4Animal physiology, Shahid Beheshti University, IR Iran
Published online:Jun 30, 2012
Article type:Research Article
Received:Jan 20, 2012
Accepted:Apr 02, 2012
How to Cite:Davod JafaripourHomayoun KhazaliHasan RokniHiva AlipanahEffect of Interaction Between Testosterone and Morphine on Serum Ghrelin Concentration in Sheep Fed on Different Dietary Energy Levels.10(3):558-562.https://doi.org/10.5812/ijem.4211.
Abstract
Objectives:
The goal of the present study was to investigate the effect of testosterone, morphine or simultaneous injection of testosterone and morphine on mean serum ghrelin concentration in sheep.
Materials and Methods:
Ten sheep were divided into two groups (n = 5 in each group), they were fed with either 50 % or 100 % of their dietary energy needs for 10 days. Body weight was measured on the 1st and 10th day of the experiment. Animals in both groups received testosterone (60 ?g/kg), morphine (0.15 mg/kg), or a simultaneous infusion of testosterone (60 ?g/kg) and morphine (0.15 mg/kg), on the 8th, 9th, or 10th day of the experiment respectively. Blood samples were collected before and 2 hours after the infusions. Ghrelin concentration was determined by RIA (radio immunoassay).
Results:
In the 50 % group, ghrelin concentrations increased significantly on the 8th day of the experiment, compared to the 1st day (P < 0.05). While in the 100 % group, no significant change was observed. In both groups the animals body weight did not increase significantly on the 10th day compared to the 1st day. Testosterone significantly increased ghrelin levels after injection compared to before infusion, in both groups (P < 0.05). Morphine increased ghrelin concentration in both groups, but this increase was not statistically significant. Simultaneous injection of testosterone and morphine together, significantly increased ghrelin concentration following injection compared to before infusion, in both groups (P < 0.05).
Conclusions:
There is a direct correlation between food restriction, testosterone and ghrelin concentration in ruminants. However, a simultaneous injection of testosterone and morphine did not exert an additive effect on ghrelin secretion.
Keywords
Copyright
© 2012, Author(s). This open-access article is available under the Creative Commons Attribution 4.0 (CC BY 4.0) International License (https://creativecommons.org/licenses/by/4.0/), which allows for unrestricted use, distribution, and reproduction in any medium, provided that the original work is properly cited.
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