Effect of Interaction Between Testosterone and Morphine on Serum Ghrelin Concentration in Sheep Fed on Different Dietary Energy Levels

authors:

avatar Davod Jafaripour 1 , * , avatar Homayoun Khazali 2 , avatar Hasan Rokni 3 , avatar Hiva Alipanah 4

Faculty of Biology Science, Shahid Beheshti University, azn_araz@yahoo.com, IR Iran
Faculty of Physiology, Shahid Beheshti University, IR Iran
Applied Scientific Education Institute of Jahad Keshavarzi, IR Iran
Animal physiology, Shahid Beheshti University, IR Iran
International Journal of Endocrinology and Metabolism: Vol.10, issue 3; 558-562
published online: June 30, 2012
article type: Original Article
received: January 20, 2012
accepted: April 2, 2012
DOI: https://doi.org/10.5812/ijem.4211

how to cite: Jafaripour D, Khazali H, Rokni H, Alipanah H. Effect of Interaction Between Testosterone and Morphine on Serum Ghrelin Concentration in Sheep Fed on Different Dietary Energy Levels. Int J Endocrinol Metab. 2012;10(3): 558-562. https://doi.org/10.5812/ijem.4211.

Abstract

Background:

Ghrelin plays an important role in the regulation of food intake and body weight. It also decreases testosterone and opioid secretion.

Objectives:

The goal of the present study was to investigate the effect of testosterone, morphine or simultaneous injection of testosterone and morphine on mean serum ghrelin concentration in sheep.

Materials and Methods:

Ten sheep were divided into two groups (n = 5 in each group), they were fed with either 50 % or 100 % of their dietary energy needs for 10 days. Body weight was measured on the 1st and 10th day of the experiment. Animals in both groups received testosterone (60 ?g/kg), morphine (0.15 mg/kg), or a simultaneous infusion of testosterone (60 ?g/kg) and morphine (0.15 mg/kg), on the 8th, 9th, or 10th day of the experiment respectively. Blood samples were collected before and 2 hours after the infusions. Ghrelin concentration was determined by RIA (radio immunoassay).

Results:

In the 50 % group, ghrelin concentrations increased significantly on the 8th day of the experiment, compared to the 1st day (P < 0.05). While in the 100 % group, no significant change was observed. In both groups the animals body weight did not increase significantly on the 10th day compared to the 1st day. Testosterone significantly increased ghrelin levels after injection compared to before infusion, in both groups (P < 0.05). Morphine increased ghrelin concentration in both groups, but this increase was not statistically significant. Simultaneous injection of testosterone and morphine together, significantly increased ghrelin concentration following injection compared to before infusion, in both groups (P < 0.05).

Conclusions:

There is a direct correlation between food restriction, testosterone and ghrelin concentration in ruminants. However, a simultaneous injection of testosterone and morphine did not exert an additive effect on ghrelin secretion.

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