Oxidative stress and proteolytic activity in erythrocytes of diabetic patients

How to Cite:Suryakanth Varashree B, Gopalakrishna BP. Oxidative stress and proteolytic activity in erythrocytes of diabetic patients. Int J Endocrinol Metab. 2010;8(2):. doi:

Abstract

Background: High blood glucose levels induce oxidative stress and decrease antioxidant defenses.
Objectives: The present study aims to assess the association, if any, between increase in proteolytic enzyme activities and oxidative stress in diabetic subjects.
Patients and Methods: Fifty-one non diabetic patients (33 men, 18 women, mean age 54.47 year) and 53 diabetic patients (39 men, 14 women, mean age 52.92year) were included in the study. Diabetic status was assessed by the fasting blood sugar (FBS) using glucose oxidase method. Oxidant stress was measured by estimating erythrocyte malondialdehyde (MDA) in terms of thiobarbituric acid reacting substance (TBARS). Proteolytic activity was estimated by the amount of amino groups released by the erythrocyte lysate of the diabetic individual using phenylhydrazine treated hemoglobin.
Results: Erythrocyte MDA was higher in diabetics (cases) (4.7±1.7 nmoles/gHb) than in controls (3.3±2.2 nmoles/gHb) (p=0.001). Erythrocyte proteolytic activity was also higher in cases (167.2±648 nmoles/gHb) than the controls (27.9±31.7 nmoles/gHb).
Conclusions: Both erythrocyte lipid peroxidation and the proteolytic activity in the erythrocyte lysates of diabetic patients was significantly higher in cases than in controls. Diabetes is associated with a significant increase in TBARS, an index of oxidant stress. Proteolytic enzymes degrade many oxidatively altered proteins preventing the accumulation of altered and damaged proteins in the cell.

Please cite this paper as:
Varashree BS, Gopalakrishna BP. Oxidative stress and proteolytic activity in erythrocytes of diabetic patients. Int J Endocrinol Metab. 2010;8(2):90-93.

Keywords

Erythrocyte

Malondialdehyde

Oxidative stress

Fulltext

Full text is available in PDF

Import into EndNote Import into BibTex

Share on

Comments

Number of Comments:0

Cited by

Scopus by DOI: 0
Last Update: 3 days ago
Scopus by Title: 4
Last Update: 3 days ago
Scopus by Title (Ref): 5
Last Update: 3 days ago
CrossRef: 0
Last Update: 2 days ago

Metrics

Ordering Reprints

Articles are published under the Creative Commons license stated on each article. No permission or royalty fee is required for uses permitted by that license. CCC handles optional bulk and customized reprint orders. Any quotation covers production and delivery services only, not copyright permission. > Request Reprints from CCC

Search Relations

Author(s):

Bolar Suryakanth Varashree:[PubMed][Scholar]
Bhat Perumunda Gopalakrishna:[PubMed][Scholar]

Research Institute for Endocrine Sciences

Outlines

Oxidative stress and proteolytic activity in erythrocytes of diabetic patients

Abstract

Fulltext

Last Update: 3 days ago

Last Update: 3 days ago

Last Update: 3 days ago

Last Update: 2 days ago