Liver and Plasma Nesfatin-1 Responses to 6 Weeks of Treadmill Running With or Without Zizyphus Jujuba Liquid Extraction in Female Rat


avatar Abbass Ghanbari Niaki 1 , * , avatar Fatemeh Mohammadi Joojadeh 1 , avatar Navabeh Zare Kookandeh 1 , avatar Safar Najafi 1 , avatar Mohammad Javad Chaichi 2 , avatar Fatemeh Rodbari 3 , avatar Hasan Bayat 4

Exercise Biochemistry Division, Department of Physical Education and Sport Sciences, University of Mazandarn, Baboulsar, IR Iran
Department of Analytical Chemistry, Faculty of Chemistry, Mazandarn University, Baboulsar, IR Iran
Department of cell and molecular biology, Faculty of Basic Sciences, University of Mazandran, Baboulsar, IR Iran
Sina Laboratory, Mazandaran, Ghaemshahr, IR Iran

how to cite: Ghanbari Niaki A, Mohammadi Joojadeh F, Zare Kookandeh N, Najafi S, Chaichi M J, et al. Liver and Plasma Nesfatin-1 Responses to 6 Weeks of Treadmill Running With or Without Zizyphus Jujuba Liquid Extraction in Female Rat. Int J Endocrinol Metab. 2013;11(2): 95-101.



Nesfatin-1 is a protein derived from a precursor molecule of the nucleobindin-2 gene, and acts as an anorexigenic peptide on food intake behavior, and its level isinfluenced by nutritional status, food composition [fat and carbohydrate (CHO)], and physical exercise.


The aim of this study was to investigate the effects of 6 weeks of treadmill running (at high intensity) program with and without zizyphus jujuba (high carbohydrate content) crud extraction on liver nesfatin-1, ATP, glycogen, and its plasma concentrations in female rats.

Materials and Methods:

Twenty-eight Wistar female rats (6-8 weeks old100-120 g of weight) were randomly assigned to saline-control (SC), saline-training (ST), zizyphus jujuba-control (ZJC), and zizyphus jujuba-training (ZJT) groups. Rats ran on a motor-driven treadmill at 35 m/min, 60 min/day, 5 days/week for 6 weeks. Animals received ZJ extraction and saline at the dose of 1.25mL/100g of body weight. Seventy-two hours after the last training session rats were killed, a portion of liver excited, and plasma was collected for nesfatin-1, ATP, and glycogen measurements. A one-way ANOVA method, and Pearson correlation were employed. P < 0.05 was considered as significant.


A higher and significant liver nesfatin-1 level was found in ZJ groups (p < 0.005), but plasma nesfatin-1 responded differently. Changes in liver nesfatin-1 were accompanied with an increase in liver glycogen,but not ATP contents.


The Findings indicate that higher liver nesfatin-1 and glycogen content by ZJ extraction might be due to the ZJ high CHO content, and it could be consideredas an anti-appetite herb.

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