The Association between PAI-1 Gene Promoter Polymorphism and Serum Serpin E1, MDA, and Hs-CRP Levels in Coronary Artery Disease

authors:

avatar Ansar Karimian 1 , avatar Safar Farajnia 2 , * , avatar Morteza Ghojazadeh 3 , avatar Fatemeh Khaki-Khatibi 4

Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
Department of Clinical Biochemistry, Faculty of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran
Liver and Gastrointestinal Disease Research center, Tabriz University of Medical Sciences, Tabriz, Iran
Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran
International Cardiovascular Research Journal: Vol.10, issue 3; e10176
published online: September 15, 2016
article type: Research Article
received: December 09, 2015
revised: February 03, 2016
accepted: March 09, 2016

how to cite: Karimian A , Farajnia S , Ghojazadeh M , Khaki-Khatibi F . The Association between PAI-1 Gene Promoter Polymorphism and Serum Serpin E1, MDA, and Hs-CRP Levels in Coronary Artery Disease. Int Cardiovasc Res J. 2016;10(3):e10176. 

Abstract

Background:
Coronary Artery Disease (CAD) is caused by atherosclerosis. Studies have shown that a number of factors, including cellular binding molecules such as Plasminogen Activator Inhibitor-1 (PAI-1), lipid peroxidation, inflammation, and hemostasis, are closely related to development and progression of CAD.
Objectives:
The present case-control study aimed to evaluate the association between Plasminogen Activator Inhibitor-1 (PAI-1) 4G/5G polymorphism and oxidative stress markers and Coronary Artery Disease (CAD).
Patients and Methods:
Blood was drawn and DNA was extracted from 90 subjects (46 patients with angiographically diagnosed CAD and 44 age- and sex-matched healthy controls). The 4G/5G polymorphism of PAI-1 was detected by Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP) analysis. Besides, the risk factors, serpin E1, Malondialdehyde (MDA), high-sensitivity C-Reactive Protein (hs-CRP), and lipid profile serum levels were measured by standard methods and were compared between the two study groups using independent samples t-test, one-way ANOVA, and Mann-Whitney U test as appropriated.
Results:
Results: The frequency of 4G/4G genotype of PAI-1 gene was higher in the CAD patients than in the controls (28/46 (60.87%) vs. 8/44 (18.18%), P < 0.01). Additionally, the serpin E1 plasma level was significantly higher in the CAD group carrying the 4G allele compared to those homozygous for the 5G allele (P = 0.016). Besides, a significant difference was found between the 4G/4G and 5G/5G subjects of the CAD group regarding plasma High-Density Lipoprotein (HDL) (P < 0.01). Also, significant differences were observed among the three genotypes of both groups concerning the plasma levels of cholesterol, triglyceride, and Low-Density Lipoprotein (LDL). However, no significant correlation was found between PAI-1 gene polymorphism and MDA serum level, hs-CRP, and risk of CAD.
Conclusion:
The findings of this study suggested that 4G/4G PAI-1 polymorphism was associated with cholesterol, triglyceride, LDL, and HDL levels and could be regarded as a biomarker for risk of CAD.
 

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