Interferon Gamma Polymorphism and Expression Relationship with Severity of Coronary Artery Disease in Golestan, Iran

Farnoosh  shateri; Touraj Farazmandfar; Ali  Sharifian; Reza   Salehi Manzari; Marzieh  Attar; Majid Shahbazi

International Cardiovascular Research Journal

The Official Annually Journal of Cardiovascular Research Center, Shiraz University of Medical Sciences

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Interferon Gamma Polymorphism and Expression Relationship with Severity of Coronary Artery Disease in Golestan, Iran

Author(s):

Farnoosh shateri¹,

Touraj Farazmandfar¹,

Ali Sharifian²,

Reza Salehi Manzari³,

Marzieh Attar¹,

Majid Shahbazi^4,*

1Medical Cellular and Molecular Research Center, Golestan University of Medical Sciences, Gorgan, Iran

2Department of Heart, Kosar Heart Center, Golestan University of Medical Sciences, Gorgan, Iran

3Resident of Internal Medicine, Faculty of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran

4Arya Tina Gene (ATG) Biophamaceutical Company, Gorgan, Iran

International Cardiovascular Research Journal:Vol. 11, issue 3; e11025

Published online:Sep 01, 2017

Article type:Research Article

Received:Aug 21, 2017

Accepted:May 11, 2017

How to Cite:Farnoosh shateriTouraj FarazmandfarAli SharifianReza Salehi ManzariMarzieh AttarMajid Shahbaziet al.Interferon Gamma Polymorphism and Expression Relationship with Severity of Coronary Artery Disease in Golestan, Iran.Int Cardiovasc Res J.11(3):e11025.

Abstract

Background: The Coronary artery disease (CAD) occurs as a result of atherosclerotic
plaque formation. The interferon gamma (IFNγ) as a multifunctional cytokine is
involved in inflammatory processes in atherosclerosis.
Objectives: We investigated the relationship between IFNγ (+874T/A) SNP with CAD.
Moreover, we compared IFNγ mRNA expression in CAD patients and healthy controls.
Patients and Methods: This case-control study with randomized sampling included
300 patients with CAD and 301 normal controls. The SSP-PCR method was used for
genotyping IFNγ (+874T/A) gene polymorphism. Quantitative Real-Time PCR was
performed to measure IFNγ mRNA expression. All data was analyzed by GraphPad
software. The chi-square and ordinal logistic regression tests were used to analyze
differences in genotype frequencies.
Results: In this study, there was a significant association between male genders with
CAD (P < 0.001). There was a significant association between genotype T/T and Allele T
of IFNγ (+874T/A) polymorphism with CAD (P = 0.021 and P = 0.022, respectively). The
inheritance model analysis showed that two copy of allele T is required for increased risk
in CAD (P = 0.031). There was a significant association between the genotype T/T of IFNγ
(+874T/A) polymorphism with CAD patients with double and triple vessel disease (P =
0.030 and P = 0.013, respectively). The IFNγ mRNA level in CAD group was significantly
higher than control group (P = 0.024).
Conclusions: Conclusions: IFNγ gene functional polymorphism can be associated with
incidence and severity of CAD. IFNγ mRNA level was also increased in CAD patients
in comparison with controls. Therefore, IFNγ may play a role in predisposition to CAD.

Keywords

Interferon-Gamma

Coronary Artery Disease

Single Nucleotide Polymorphism

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