Differential Expression of Let-7 MicroRNA in Patients with Coronary Artery Disease: Possible Potential for Early Diagnosis, Treatment, and Prevention

Mohammad Javad Zibaeenezhad; Ali Karimi Akhormeh; Mahintaj Dara; Zeinab Jamali; Iman Razeghian-Jahromi

International Cardiovascular Research Journal

The Official Annually Journal of Cardiovascular Research Center, Shiraz University of Medical Sciences

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Differential Expression of Let-7 MicroRNA in Patients with Coronary Artery Disease: Possible Potential for Early Diagnosis, Treatment, and Prevention

Author(s):

Mohammad Javad Zibaeenezhad

¹,

Ali Karimi Akhormeh

¹,

Mahintaj Dara

²,

Zeinab Jamali³,

Iman Razeghian-Jahromi

^1,*

1Cardiovascular Research Center, Shiraz University of Medical Sciences, Shiraz, IR Iran

2Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran

3Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, IR Iran

International Cardiovascular Research Journal:Vol. 17, issue 2; e141307

Published online:Jun 30, 2023

Article type:Research Article

Received:Sep 20, 2023

Accepted:Nov 27, 2023

How to Cite:Mohammad Javad ZibaeenezhadAli Karimi AkhormehMahintaj DaraZeinab JamaliIman Razeghian-JahromiDifferential Expression of Let-7 MicroRNA in Patients with Coronary Artery Disease: Possible Potential for Early Diagnosis, Treatment, and Prevention.Int Cardiovasc Res J.17(2):e141307.

Abstract

Background: Coronary artery disease (CAD) and its numerous consequences significantly reduce the quality of life for millions of people worldwide.

Objectives: This study investigated the differential expression of let-7 microRNA between CAD patients and healthy peers, which can be used as an early diagnostic, therapeutic, and preventive tool.

Methods: In this case-control study, participants were selected through convenient sampling and categorized into CAD-positive (n = 25) and CAD-negative (n = 25) groups. Blood samples were obtained, and the level of let-7 miRNA was measured. Various other parameters, including demographic variables, blood pressure, and results of routine blood tests besides smoking status, educational level, overweight, diabetes status, and history of coronary atherosclerosis, were recorded. Familial history of hypertension, diabetes, atherosclerosis, and sudden death were also registered. Different statistical methods including independent sample t, Mann-Whitney, and chi-squared tests were used to compare variables. A P value of less than 0.05 was considered significant.

Results: Patients with CAD were older, with a higher frequency of the male sex. Fasting blood sugar (P = 0.002), triglyceride (P < 0.001), HDL-cholesterol (P = 0.038), triglyceride-glucose index (P < 0.001), TG/HDL-cholesterol ratio (P < 0.001), and LDL/HDL ratio (P = 0.011) were significantly higher in the case group. Also, smoking (P = 0.001), illiteracy (P = 0.005), dyslipidemia (P = 0.048), and a history of coronary atherosclerosis (P = 0.022) were more prevalent in the CAD patients. Differential expression of let-7 microRNA between groups was at the borderline of statistical significance (P = 0.058).

Conclusion: Let-7 microRNA was expressed higher in patients with CAD, which may be helpful in the early diagnosis of atherosclerotic plaques and can possibly be used in designing therapeutic and preventive approaches.

Keywords

Myocardial Infarction

Coronary Artery Disease

Non-Coding RNA

MicroRNA

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