Differential Expression of Let-7 MicroRNA in Patients with Coronary Artery Disease: Possible Potential for Early Diagnosis, Treatment, and Prevention

authors:

avatar Mohammad Javad Zibaeenezhad ORCID 1 , avatar Ali Karimi Akhormeh ORCID 1 , avatar Mahintaj Dara ORCID 2 , avatar Zeinab Jamali 3 , avatar Iman Razeghian-Jahromi ORCID 1 , *

Cardiovascular Research Center, Shiraz University of Medical Sciences, Shiraz, IR Iran
Stem Cells Technology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran
Department of Medical Genetics, School of Medicine, Tehran University of Medical Sciences, Tehran, IR Iran
International Cardiovascular Research Journal: Vol.17, issue 2; e141307
published online: June 30, 2023
article type: Research Article
received: September 20, 2023
revised: November 06, 2023
accepted: November 27, 2023

how to cite: Zibaeenezhad M J, Karimi Akhormeh A, Dara M, Jamali Z, Razeghian-Jahromi I. Differential Expression of Let-7 MicroRNA in Patients with Coronary Artery Disease: Possible Potential for Early Diagnosis, Treatment, and Prevention. Int Cardiovasc Res J. 2023;17(2):e141307. 

Abstract

Background: Coronary artery disease (CAD) and its numerous consequences significantly reduce the quality of life for millions of people worldwide.

Objectives: This study investigated the differential expression of let-7 microRNA between CAD patients and healthy peers, which can be used as an early diagnostic, therapeutic, and preventive tool.

Methods: In this case-control study, participants were selected through convenient sampling and categorized into CAD-positive (n = 25) and CAD-negative (n = 25) groups. Blood samples were obtained, and the level of let-7 miRNA was measured. Various other parameters, including demographic variables, blood pressure, and results of routine blood tests besides smoking status, educational level, overweight, diabetes status, and history of coronary atherosclerosis, were recorded. Familial history of hypertension, diabetes, atherosclerosis, and sudden death were also registered. Different statistical methods including independent sample t, Mann-Whitney, and chi-squared tests were used to compare variables. A P value of less than 0.05 was considered significant.

Results: Patients with CAD were older, with a higher frequency of the male sex. Fasting blood sugar (P = 0.002), triglyceride (P < 0.001), HDL-cholesterol (P = 0.038), triglyceride-glucose index (P < 0.001), TG/HDL-cholesterol ratio (P < 0.001), and LDL/HDL ratio (P = 0.011) were significantly higher in the case group. Also, smoking (P = 0.001), illiteracy (P = 0.005), dyslipidemia (P = 0.048), and a history of coronary atherosclerosis (P = 0.022) were more prevalent in the CAD patients. Differential expression of let-7 microRNA between groups was at the borderline of statistical significance (P = 0.058).

Conclusion: Let-7 microRNA was expressed higher in patients with CAD, which may be helpful in the early diagnosis of atherosclerotic plaques and can possibly be used in designing therapeutic and preventive approaches.

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References

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