Expression of miR21, miR122, miR146a and miR196 in Symptomatic Carotid Disease

Anna Sioziou; Hector Katifelis; Evangelia Legaki; Nikolaos  Patelis; Dimitrios Athanasiadis; Theodoros Liakakos; Christos Klonaris; Maria Gazouli

International Cardiovascular Research Journal

The Official Annually Journal of Cardiovascular Research Center, Shiraz University of Medical Sciences

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Expression of miR21, miR122, miR146a and miR196 in Symptomatic Carotid Disease

Author(s):

Anna Sioziou¹,

Hector Katifelis¹,

Evangelia Legaki¹,

Nikolaos Patelis²,

Dimitrios Athanasiadis²,

Theodoros Liakakos²,

Christos Klonaris²,

Maria Gazouli^1,*

1Department of Basic Medical Sciences, Laboratory of Biology, Medical School, National and Kapodistrian University of Athens, Athens, Greece

2First Department of Surgery, Vascular Unit, National and Kapodistrian University of Athens, Laiko General Hospital, Athens, Greece

International Cardiovascular Research Journal:Vol. 12, issue 1; e62057

Published online:Mar 01, 2018

Article type:Research Article

Received:Feb 12, 2018

Accepted:Dec 13, 2017

How to Cite:Anna SioziouHector KatifelisEvangelia LegakiNikolaos PatelisDimitrios AthanasiadisTheodoros LiakakosChristos KlonarisMaria Gazouliet al.Expression of miR21, miR122, miR146a and miR196 in Symptomatic Carotid Disease.Int Cardiovasc Res J.12(1):e62057.

Abstract

Background: Carotid disease is one of the many forms of cardiovascular disease, which
may lead to chronic disability and death. It is a multifactorial inflammatory disease,
greatly affected by an individual’s habits like smoking, lack of exercise, and a diet high in
fats. MicroRNAs (miRs) are known to be involved in vascular inflammation.
Objectives: We aimed to analyse in a case-control study the expression profile of selected
miRs from patients with symptomatic carotid disease and to examine their involvement
in the disease pathogenesis.
Patients and Methods: Samples from 38 symptomatic patients who underwent carotid
endarterectomy were collected and adjacent healthy regions from 15 patients were used
as control samples. Fold change in the expression of miR21, miR122, miR146a and
miR196α was measured using reverse transcription-real time PCR. Western blot was
used to quantify the levels of MMP2 protein whose gene is a target of miR21.
Results: Compared to control samples, all patients showed upregulation of miR21,
miR122, miR146a and miR196a. No statistical significance was found to exist from
patients with high or low miRs expression and clinical/laboratory parameters. The levels
of MMP2 were found to be decreased in patients when compared to control samples.
Conclusions: Our results revealed miRs which showed different expression in
endarterectomy specimens from patients with symptomatic carotid disease, suggesting
that these miRs correlated with vascular inflammation. Furthermore, mir21 seems an
appealing pharmaceutical target since by targeting MMP2 can favour a stable plaque
since low levels of the protein of its gene MMP2 target prevent the fibrous cap of the
atheroma from getting thinner. Thus, miR21 seems to prevent rupture but further
research is required.

Keywords

Carotid Artery Diseases

MicroRNAs

Inflammation

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