The rapid method of preparing mouse karyotype and mouse chromosomes identified by Gimsa staining and Trypsin-Gimsa banding

E Mirabzadeh Ardakani

Journal of Inflammatory Diseases

The Official Journal of Qazvin University of Medical Sciences

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The rapid method of preparing mouse karyotype and mouse chromosomes identified by Gimsa staining and Trypsin-Gimsa banding

Author(s):

E Mirabzadeh Ardakani^,*

Journal of Inflammatory Diseases:Vol. 4, issue 4; 14-22

Article type:Research Article

How to Cite:E Mirabzadeh ArdakaniThe rapid method of preparing mouse karyotype and mouse chromosomes identified by Gimsa staining and Trypsin-Gimsa banding.J Inflamm Dis.4(4):e154789.

Abstract

Abstract Background: Preparation and staining of mouse chromosomes by Gimsa and T-G banding is the cornerstone of many researches on diagnosis, prevention and treatment of many diseases, production of new drugs and quality control of biological derivatives. Objective: To provide a sample karyotype of mouse. Methods: Mouse chromosomes were prepared from bone marrow of male outbred mice (NMRI). After culturing and preparing the slides, they were studied by staining with Gimsa and Trypsin-Gimsa (T-G) banding techniques. Karyotypes were prepared according to the standard karyotype of the mouse. Findings: Metaphase with spread from diploid cells contained 40 chromosomes and all of them were characterized by their banding patterns. Conclusion: T-G technique provides greather detail and demonstrates more bands, in contrast with other methods.

Keywords

Keywords: Chromosome

Karyotype

Trypsin-Gimsa (T-G) banding

Mouse

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