Effect of zinc on viability and morphology of Raji cell-line

H Tokmehdashi; F Osati-Ashtiani; AA Pourfathullah

Journal of Inflammatory Diseases

The Official Journal of Qazvin University of Medical Sciences

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Effect of zinc on viability and morphology of Raji cell-line

Author(s):

H Tokmehdashi^,*,

F Osati-Ashtiani,

AA Pourfathullah

Journal of Inflammatory Diseases:Vol. 8, issue 3; 3-7

Article type:Research Article

How to Cite:H TokmehdashiF Osati-AshtianiAA PourfathullahEffect of zinc on viability and morphology of Raji cell-line.J Inflamm Dis.8(3):e155061.

Abstract

¯Abstract Background: Zinc has important effects on structural and functional activities of many proteins and enzymes, specially regulation of immune system. Objective: This study was carried out to examine the in vitro effects of different concentration of zinc on viability and morphology of Raji cell line. Methods: In this study the cell line was exposed to different concentration of zinc(10nM to 500µM)followed by incubation (37 c, 5%Co2) at various time points(12 to 72 hrs). The cells were then evaluated with trypan blue exclusion dye , and Wright-Gimsa staining. Findings: The results showed almost different responses to different amount of zinc by the Raji cells. less than 100µM at different incubation time points had no effects on cell line when compared to the controls. Higher concentrations of zinc (>100µM) viability diminished to 70% at 12 hrs and less than 50% at 24 hrs of incubation times . Conclusion: We conclude that Zn has dose-dependent cytotoxic effect on Raji cells and probably application for immune-modulation.

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