Anticonvulsant activity of ethanolic extract and aqueous fraction of Launaea acanthodes gum in comparison with diazepam in mice

A Karimidokht shahrbabaki; Sh Oryan; K Parivar

Journal of Inflammatory Diseases

The Official Journal of Qazvin University of Medical Sciences

Current Issue All Issues In Press Accepted Manuscripts Search

Journal Information Editors & Boards Indexing and Listing Sources Reviewer and AE Registration Form Journal Metrics Open Peer Review (OPR)Publication Ethics and Publication Malpractice Statement Support Contact Us

Authors Guide Submit Manuscript

Anticonvulsant activity of ethanolic extract and aqueous fraction of Launaea acanthodes gum in comparison with diazepam in mice

Author(s):

A Karimidokht shahrbabaki^,*,

Sh Oryan,

K Parivar

Journal of Inflammatory Diseases:Vol. 13, issue 1; 14-20

Article type:Research Article

How to Cite:A Karimidokht shahrbabakiSh OryanK ParivarAnticonvulsant activity of ethanolic extract and aqueous fraction of Launaea acanthodes gum in comparison with diazepam in mice.J Inflamm Dis.13(1):e155447.

Abstract

Background: Launaea acanthodes gum (LAG) contains flavonoids with benzodiazepine-like activity and so it may be helpful in treatment of epilepsy. Objective: To determine the effects of ethanolic extract (EE) and aqueous fraction (AF) of LAG on convulsion induced by pentylentetrazole (PTZ) in mice. Methods: This experimental study was carried out at the Department of Biology, Science and Research Division, Azad University, Tehran (Iran) in 2005. Lethal doses (LD50) of EE and AF were determined by acute toxicity test. The effect of AF on activity of brain was investigated by using open filed test and the signs (rearing, locomotion) were compared with control group. Later, the animals in experimental groups (10 mice) received different doses of EE (100, 200, 300 mg/kg) and AF (200, 300,400 mg/kg) via intraperitoneal injections 30 min prior to PTZ injection. The members of control group received saline and those in positive control group were given diazepam (10 mg/kg). Then the epileptiform behaviors were investigated following the subcutaneous injection of PTZ (85 mg/kg) for 30 minutes. Findings: In the open filed test, a single dose of AF exhibited a significant decrease in rearing with no such effect on locomotion activity. Also, different doses of EE and AF inhibited convulsions through an increase in latency to the onset of forelimb clonus and tonic-clonic seizures. Conclusion: According to our data, the LAG extracts have anticonvulsant and anxiolytic activities with no obvious sing of depression.

Keywords

comments