Abstract
Background: There are reports that essential oils of different species of eucalyptus have special properties including anti-bacterial, antiviral, anticancer and insecticidal activities. Most of plant essential oils are dissolved in polar or non-polar solvents before examination. Objective: The aim of this study was to determine the cytotoxic effects of different solvents and essential oil of eucalyptus on human fibroblast cells. Methods: This experimental study was performed in the Institute of Biotechnology affiliated to Iranian Research Organization for Science and Technology during 2013-2014. The studied solvents were including ethanol, propylene glycol, dichloromethane, and dimethyl sulfoxide. Fibroblast cells were seeded in 96-well plates. After 24 h incubation at 37ºC, the cells were treated with different concentrations of the solvents or essential oil of eucalyptus (0.05-1 mg/ml). The viability of the cells was determined by the colorimetric MTT assay. Data were analyzed using T-test and One-way ANOVA. Findings: The lowest cytotoxic effect against fibroblast cells was related to 1% ethanol and propylene glycol, while the highest cytotoxic effect was related to 10% dimethyl sulfoxide that decreased cell survival by 47%. Essential oil of eucalyptus also induced fibroblast cell death at concentrations higher than 0.05 mg/ml. Conclusion: For clinical application of essential oil of eucalyptus, using solvent with appropriate concentration is necessary to prevent its cytotoxic effects.
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Copyright
© 2024, Journal of Inflammatory Diseases. This open-access article is available under the Creative Commons Attribution-NonCommercial 4.0 (CC BY-NC 4.0) International License (https://creativecommons.org/licenses/by-nc/4.0/), which allows for the copying and redistribution of the material only for noncommercial purposes, provided that the original work is properly cited.