Effect of Helicobacter pylori, black tea and sodium bicarbonate on iron metabolism and MDCK cell survival

Dariush Hamedi Asl; babak rahmani; Taghi Naserpour Farivar; Fatemeh Haj Manoochehri; Amir Peymani

Journal of Inflammatory Diseases

The Official Journal of Qazvin University of Medical Sciences

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Effect of Helicobacter pylori, black tea and sodium bicarbonate on iron metabolism and MDCK cell survival

Author(s):

Dariush Hamedi Asl¹,

babak rahmani¹,

Taghi Naserpour Farivar²,

Fatemeh Haj Manoochehri³,

Amir Peymani^2,*

1Department of Molecular Medicine, Faculty of Medical Sciences, Qazvin University of Medical Sciences, Qazvin, Iran

2Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran

3Department of Pathology, Qazvin University of Medical Sciences, Qazvin, Iran

Journal of Inflammatory Diseases:Vol. 22, issue 5; 36-43

Article type:Research Article

How to Cite:Dariush Hamedi Aslbabak rahmaniTaghi Naserpour FarivarFatemeh Haj ManoochehriAmir PeymaniEffect of Helicobacter pylori, black tea and sodium bicarbonate on iron metabolism and MDCK cell survival.J Inflamm Dis.22(5):e156111.

Abstract

Background: Iron deficiency anemia is the most common nutritional disorder in the world. Diet and Helicobacter pylori infection are among the main causes of this disorder. Objective: In this study, the effect of black tea extract and sodium bicarbonate with Helicobacter pylori on the genes involved in iron absorption and storage, as well as cell proliferation, were studied. Methods: Simultaneous cultivation of MDCK and Helicobacter pylori cell lines was performed at concentrations of 10, 20, 40 and 80 μg/ml of tea extract and 30, 40, 60 and 100 mM sodium bicarbonate at 24 and 48 hours. The effect of treatment on cell survival was investigated by trypan blue staining and expression of MYC, TFRC, FTH1, IRP2, IRP1, and NDRG1 genes by real-time PCR and analyzed by ANOVA and independent T-test. Findings: There was no significant change in the expression of the genes involved in iron metabolism under the influence of tea, sodium bicarbonate and Helicobacter pylori treatment in MDCK cell line. Upregulation MYC gene expression was observed in the presence of Helicobacter pylori after 24 hours treatment with tea extract, and sodium bicarbonate, and in the absence of Helicobacter pylori upregulation with tea extract after 48 hours (P

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